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BAX Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249645

BAX Antibody, BCL2-associated X protein Antibody, Bcl2-L-4 Antibody, Apoptosis regulator BAX Antibody, Bcl-2-like protein 4 Antibody, BCL2L4 Antibody Description: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
BAX, Polyclonal Antibody
Full Product Name
Anti-BAX Antibody (BH3 Domain) IHC-plus
Product Gene Name
Product Synonym Names
Rabbit Polyclonal to Human BAX; Human BAX; BCL2-associated X protein; Bcl2-L-4; Apoptosis regulator BAX; Bcl-2-like protein 4; BCL2L4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Ammonium sulfate precipitation
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.
Molecular Weight
19,718 Da
OMIM
600040
3D Structure
ModBase 3D Structure for Q07812
Immunogen
BAX antibody was raised against tRKC recombinant protein.
Immunogen Description
TRKC recombinant protein.
Immunogen Type
Recombinant protein
Antigen Modification
BH3 Domain
Target Species
Human
Applications Tested/Suitable for anti-BAX antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-BAX antibody
Flow Cytometry (1:10 - 1:50)
IHC-P (1:100)
WB (1:1000)

Immunohistochemistry (IHC) of anti-BAX antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Rabbit Anti-BAX Polyclonal Antibody – Cat# MBS249645

Western Blot (WB) of anti-BAX antibody

Western blot of Bax (arrow) using rabbit polyclonal Bax Antibody (BH3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bax gene.
Rabbit Anti-BAX Polyclonal Antibody, Western Blot (WB) – Cat# MBS249645

Western Blot (WB) of anti-BAX antibody

Bax Antibody(BH3)western blot of HL-60 cell line lysates (35 ug/lane). The Bax antibody detected the Bax protein (arrow).
Rabbit Anti-BAX Polyclonal Antibody, Western Blot (WB) – Cat# MBS249645

Flow Cytometry (FC/FACS) of anti-BAX antibody

Flow cytometric of HepG2 cells using Bax Antibody (BH3 Domain Specific)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-BAX Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS249645

Other Notes

Small volumes of anti-BAX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BAX purchase

MBS249645 is an antibody targeting BAX. Also known as Anti-BAX Antibody (BH3 Domain) IHC-plus. Reactive in Mouse, Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BAX. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
apoptosis regulator BAX isoform beta
NCBI Official Synonym Full Names
BCL2-associated X protein
NCBI Official Symbol
BAX
NCBI Official Synonym Symbols
BCL2L4
UniProt Protein Name
Apoptosis regulator BAX
UniProt Synonym Protein Names
Bcl-2-like protein 4; Bcl2-L-4
Protein Family
UniProt Gene Name
BAX
UniProt Synonym Gene Names
BCL2L4; Bcl2-L-4
UniProt Entry Name
BAX_HUMAN
NCBI Protein Information
apoptosis regulator BAX

NCBI Summary for BAX

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for BAX

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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