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BLK Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6465376

MBS6465376 is an antibody targeting BLK. Also known as BLK, NT (Tyrosine-protein Kinase Blk, B Lymphoid Tyrosine Kinase, p55-Blk) (FITC). Reactive in Human, Mouse. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BLK, Polyclonal Antibody
Full Product Name
BLK, NT (Tyrosine-protein Kinase Blk, B Lymphoid Tyrosine Kinase, p55-Blk) (FITC)
Product Gene Name
anti-BLK antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Specificity
Recognizes human BLK. Species Crossreactivity: mouse
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
57,706 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (11351521..11422108). Location: 8p23-p22
OMIM
191305
3D Structure
ModBase 3D Structure for P51451
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human BLK, UniProt Accession #P54151.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-BLK antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-BLK antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-BLK antibody
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-BLK antibody

Western Blot analysis of MBS630698 in HL-60 cell line lysates (35ug/lane).This demonstrates the BLK antibody detected the BLK protein (arrow).
Rabbit Anti-BLK Polyclonal Antibody – Cat# MBS6465376

Western Blot (WB) of anti-BLK antibody

Western Blot analysis of MBS630698 in mouse kidney tissue lysate. BLK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Rabbit Anti-BLK Polyclonal Antibody, Western Blot (WB) – Cat# MBS6465376

Western Blot (WB) of anti-BLK antibody

Western Blot analysis of MBS630698 transiently transfected HEK-293 cell line lysate (1ug/lane). BLK pab(arrow) was detected using the purified Pab.
Rabbit Anti-BLK Polyclonal Antibody, Western Blot (WB) – Cat# MBS6465376

Immunohistochemistry (IHC) of anti-BLK antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS630698, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-BLK Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6465376

Product References and Citations for anti-BLK antibody

1. Islam, K.B., et al., J. Immunol. 154(3):1265-1272 (1995). 2. Drebin, J.A., et al., Oncogene 10(3):477-486 (1995).

Other Notes

Small volumes of anti-BLK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BLK. It may not necessarily be applicable to this product.

NCBI GI #
33469982
NCBI GeneID
640
NCBI Accession #
NP_001706.2
UniProt Primary Accession #
P51451
UniProt Secondary Accession #
Q16291; Q96IN1
UniProt Related Accession #
P51451
GenBank Nucleotide #
NM_001715.2
NCBI Official Full Name
tyrosine-protein kinase Blk
NCBI Official Synonym Full Names
B lymphoid tyrosine kinase
NCBI Official Symbol
BLK
NCBI Official Synonym Symbols
MODY11
UniProt Protein Name
Tyrosine-protein kinase Blk
UniProt Synonym Protein Names
B lymphocyte kinase; p55-Blk
Protein Family
Tyrosine-protein kinase
UniProt Gene Name
BLK
UniProt Entry Name
BLK_HUMAN
NCBI Protein Information
tyrosine-protein kinase Blk; p55-Blk; b lymphocyte kinase; BLK nonreceptor tyrosine kinase

NCBI Summary for BLK

This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010]
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UniProt Comments for BLK

BLK: a nonreceptor tyrosine kinase of the Src family. Expressed predominantly in B lymphocytes

Protein type: Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; TK group; Src family

Chromosomal Location of Human Ortholog: 8p23-p22

Cellular Component: extrinsic to internal side of plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: B cell receptor signaling pathway; innate immune response; positive regulation of insulin secretion; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation; T cell differentiation

Disease: Maturity-onset Diabetes Of The Young, Type 11
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Research Articles on BLK

1. Blk allele expression differences at the protein level are restricted to early B cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BLK antibody

 Reference Product  PubMed Publications
 BCR antibody  >18 publications with BLK and BCR
 CD79A antibody  >5 publications with BLK and CD79A
 CD79B antibody  >4 publications with BLK and CD79B
 FCGR2B antibody  >2 publications with BLK and FCGR2B
 FCGR2A antibody  >2 publications with BLK and FCGR2A
 PLCG2 antibody  >1 publications with BLK and PLCG2
 CBL antibody  >1 publications with BLK and CBL
 BCL2 antibody  >1 publications with BLK and BCL2

Pathways associated with anti-BLK antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway antibodies  Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Class I PI3K Signaling Events Pathway antibodies  Class I PI3K Signaling Events Pathway Diagram
 EPHA Forward Signaling Pathway antibodies  EPHA Forward Signaling Pathway Diagram
 Ephrin B Reverse Signaling Pathway antibodies  Ephrin B Reverse Signaling Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram

Diseases associated with anti-BLK antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >64 publications with BLK and Immune System Diseases
 Neoplasms Antibodies  >56 publications with BLK and Neoplasms
 Lupus Erythematosus, Systemic Antibodies  >38 publications with BLK and Lupus Erythematosus, Systemic
 Nervous System Diseases Antibodies  >19 publications with BLK and Nervous System Diseases
 Arthritis, Rheumatoid Antibodies  >17 publications with BLK and Arthritis, Rheumatoid
 Cardiovascular Diseases Antibodies  >15 publications with BLK and Cardiovascular Diseases
 Brain Diseases Antibodies  >14 publications with BLK and Brain Diseases
 Necrosis Antibodies  >13 publications with BLK and Necrosis
 Inflammation Antibodies  >12 publications with BLK and Inflammation
 Drug Toxicity Antibodies  >10 publications with BLK and Drug Toxicity

Organs/Tissues associated with anti-BLK antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >127 publications with BLK and Blood
 Spleen Antibodies  >15 publications with BLK and Spleen
 Lung Antibodies  >12 publications with BLK and Lung
 Bone Marrow Antibodies  >10 publications with BLK and Bone Marrow
 Lymph Node Antibodies  >6 publications with BLK and Lymph Node
 Lymph Antibodies  >6 publications with BLK and Lymph
 Thymus Antibodies  >5 publications with BLK and Thymus
 Intestine Antibodies  >3 publications with BLK and Intestine
 Connective Tissue Antibodies  >3 publications with BLK and Connective Tissue
 Mammary Gland Antibodies  >2 publications with BLK and Mammary Gland
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