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CDCA3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9211424

CDCA3 is a F-box-like protein which is required for entry into mitosis. It acts by participating to E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase. More details

$240 / 0.08 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CDCA3, Polyclonal Antibody
Full Product Name
CDCA3 Antibody (N-term)
Product Gene Name
Product Synonym Names
Cell division cycle-associated protein 3; Gene-rich cluster protein C8; Trigger of mitotic entry protein 1; TOME-1; CDCA3; C8; GRCC8; TOME1
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human, mouse
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Concentration
Vial Concentration: 2 (lot specific)
Specificity
This CDCA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-69 amino acids from the N-terminal region of human CDCA3.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
CDCA3 peptide (MBS9226200) is used for blocking the activity of CDCA3 antibody (MBS9211424)
Molecular Weight
28998
Immunogen Sequence Positions
41-69
OMIM
607749
3D Structure
ModBase 3D Structure for Q99618
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN

Product Categories/Family for anti-CDCA3 antibody

Applications Tested/Suitable for anti-CDCA3 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-CDCA3 antibody
WB~~1:1000

Western Blot (WB) of anti-CDCA3 antibody

Western blot analysis of CDCA3 antibody (N-term) in mouse spleen tissue lysates(35ug/lane). CDCA3 (arrow) was detected using the purified Pab.
Rabbit Anti-CDCA3 Polyclonal Antibody – Cat# MBS9211424

Immunohistochemistry (IHC) of anti-CDCA3 antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with CDCA3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-CDCA3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9211424

Flow Cytometry (FC/FACS) of anti-CDCA3 antibody

CDCA3 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CDCA3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS9211424

Product References and Citations for anti-CDCA3 antibody

Daub H., Mol. Cell 31:438-448(2008)

Other Notes

Small volumes of anti-CDCA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDCA3 purchase

MBS9211424 is an antibody targeting CDCA3. Also known as CDCA3 Antibody (N-term). Reactive in Human, mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CDCA3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cell division cycle-associated protein 3 isoform 1
NCBI Official Synonym Full Names
cell division cycle associated 3
NCBI Official Symbol
CDCA3
NCBI Official Synonym Symbols
GRCC8; TOME-1
UniProt Protein Name
Cell division cycle-associated protein 3
UniProt Synonym Protein Names
Gene-rich cluster protein C8; Trigger of mitotic entry protein 1; TOME-1
UniProt Gene Name
CDCA3
UniProt Synonym Gene Names
C8; GRCC8; TOME1; TOME-1
UniProt Entry Name
CDCA3_HUMAN
NCBI Protein Information
cell division cycle-associated protein 3

UniProt Comments for CDCA3

CDCA3: F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytosol

Biological Process: mitosis; cell division; protein ubiquitination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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