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CENPM Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9603845

MBS9603845 is an antibody targeting CENPM. Also known as CENPM Antibody. Reactive in Human, Mouse. Host: Rabbit. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CENPM, Polyclonal Antibody
Full Product Name
CENPM Antibody
Product Gene Name
anti-CENPM antibody
Product Synonym Names
bK250D10.2; C22orf18; CENP M; Centromere protein M; ICEN 39; ICEN39; Interphase centromere complex protein 39; MGC861; OTTHUMP00000028741; PANE 1; PANE1; Proliferation associated nuclear element 1; Proliferation associated nuclear element protein 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Peptide affinity purification
Concentration
1mg/ml (lot specific)
Specificity
CENPM antibody detects endogenous levels of total CENPM
Preparation and Storage
Store at -20 °C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
CENPM peptide (MBS9622563) is used for blocking the activity of CENPM antibody (MBS9603845)
Molecular Weight
20 kDa
3D Structure
ModBase 3D Structure for Q9NSP4
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human CENPM, corresponding to a region within the internal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CENPM antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-CENPM antibody
WB 1:500-1:2000
IHC 1:50-1:200
ELISA(peptide) 1:20000-1:40000

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
The optimal dilutions should be determined by the end user.

Immunohistochemistry (IHC) of anti-CENPM antibody

MBS9603845 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-CENPM Polyclonal Antibody – Cat# MBS9603845

Western Blot (WB) of anti-CENPM antibody

Western blot analysis of CENPM expression in K-562 cell lysate at 25ug/lane, The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-CENPM Polyclonal Antibody, Western Blot (WB) – Cat# MBS9603845

Other Notes

Small volumes of anti-CENPM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CENPM. It may not necessarily be applicable to this product.

NCBI GI #
50845414
NCBI GeneID
79019
NCBI Accession #
NP_001002876.1
UniProt Primary Accession #
Q9NSP4
UniProt Secondary Accession #
Q6I9W3; A7LM22; B1AHQ9
UniProt Related Accession #
Q9NSP4
GenBank Nucleotide #
NM_001002876.2
NCBI Official Full Name
centromere protein M isoform b
NCBI Official Synonym Full Names
centromere protein M
NCBI Official Symbol
CENPM
NCBI Official Synonym Symbols
PANE1; CENP-M; C22orf18
UniProt Protein Name
Centromere protein M
UniProt Synonym Protein Names
Interphase centromere complex protein 39; Proliferation-associated nuclear element protein 1
Protein Family
Centromere protein
UniProt Gene Name
CENPM
UniProt Synonym Gene Names
C22orf18; ICEN39; PANE1; CENP-M
NCBI Protein Information
centromere protein M

NCBI Summary for CENPM

The protein encoded by this gene is an inner protein of the kinetochore, the multi-protein complex that binds spindle microtubules to regulate chromosome segregation during cell division. It belongs to the constitutive centromere-associated network protein group, whose members interact with outer kinetochore proteins and help to maintain centromere identity at each cell division cycle. The protein is structurally related to GTPases but cannot bind guanosine triphosphate. A point mutation that affects interaction with another constitutive centromere-associated network protein, CENP-I, impairs kinetochore assembly and chromosome alignment, suggesting that it is required for kinetochore formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
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UniProt Comments for CENPM

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres.
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Research Articles on CENPM

1. CENP-M is crucially required for the assembly and stability of a tetramer also comprising CENP-I, CENP-H, and CENP-K, the HIKM complex, which we extensively characterize through a combination of structural, biochemical, and cell biological approaches.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CENPM antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway antibodies  Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic Anaphase Pathway antibodies  Mitotic Anaphase Pathway Diagram
 Mitotic Metaphase And Anaphase Pathway antibodies  Mitotic Metaphase And Anaphase Pathway Diagram
 Mitotic Prometaphase Pathway antibodies  Mitotic Prometaphase Pathway Diagram
 Nucleosome Assembly Pathway antibodies  Nucleosome Assembly Pathway Diagram
 Resolution Of Sister Chromatid Cohesion Pathway antibodies  Resolution Of Sister Chromatid Cohesion Pathway Diagram
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