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CHAMP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904418

Chromosome alignment-maintaining phosphoprotein (CHAMP1) is required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. It is involved in the maintenance of spindle microtubules attachment to the kinetochore during… More details

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Product Overview

Online Datasheet in PDF
Product Name
CHAMP1, Polyclonal Antibody
Full Product Name
Rabbit anti-CHAMP1 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
MRD40; zinc finger protein 828; ZNF828; CHAMP; C13orf8; chromosome alignment-maintaining phosphoprotein 1; CAMP; Chromosome alignment-maintaining phosphoprotein 1; chromosome alignment maintaining phosphoprotein 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
616327
3D Structure
ModBase 3D Structure for Q96JM3
Immunogen
Between 762 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-CHAMP1 antibody

Applications Tested/Suitable for anti-CHAMP1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for anti-CHAMP1 antibody
WB: 1:1000-1:5000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ICC: 1:250-1:1000

Immunohistochemistry (IHC) of anti-CHAMP1 antibody

Detection of human CHAMP1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CHAMP1 (Cat. No. MBS3904418 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-CHAMP1 Polyclonal Antibody – Cat# MBS3904418

Immunoprecipitation (IP) of anti-CHAMP1 antibody

Detection of human CHAMP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-CHAMP1 antibody MBS3904418 (lot MBS3904418-1) used for IP at 6 ug/mg lysate. CHAMP1 was also immunoprecipitated by rabbit anti-CHAMP1 antibody MBS3904417. For blotting immunoprecipitated CHAMP1, MBS3904418 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-CHAMP1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3904418

Western Blot (WB) of anti-CHAMP1 antibody

Detection of human and mouse CHAMP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-CHAMP1 antibody MBS3904418 (lot MBS3904418-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-CHAMP1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904418

Other Notes

Small volumes of anti-CHAMP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHAMP1 purchase

MBS3904418 is an antibody targeting CHAMP1. Also known as Rabbit anti-CHAMP1 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CHAMP1. It may not necessarily be applicable to this product.

NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Protein Name
Chromosome alignment-maintaining phosphoprotein 1
UniProt Synonym Protein Names
Zinc finger protein 828
UniProt Gene Name
CHAMP1
UniProt Synonym Gene Names
C13orf8; CAMP; CHAMP; KIAA1802; ZNF828
UniProt Entry Name
CHAP1_HUMAN

UniProt Comments for CHAMP1

ZNF828: Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.

Protein type: RNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: nucleoplasm; cytoplasm; condensed chromosome; spindle; nucleus

Molecular Function: protein binding; nucleic acid binding; metal ion binding

Biological Process: sister chromatid biorientation; attachment of spindle microtubules to kinetochore during mitosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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