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DDIT3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6291271

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein),… More details

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Product Overview

Online Datasheet in PDF
Product Name
DDIT3, Polyclonal Antibody
Full Product Name
DDIT3, CT (A135) (DDIT3, CHOP, CHOP10, GADD153, DNA damage-inducible transcript 3 protein, C/EBP-homologous protein, C/EBP-homologous protein 10, Growth arrest and DNA damage-inducible protein GADD153) (MaxLight 405)
Product Gene Name
anti-DDIT3 antibody
Product Synonym Names
DDIT3; CHOP; CHOP10; GADD153; DNA damage-inducible transcript 3 protein; C/EBP-homologous protein; C/EBP-homologous protein 10; Growth arrest and DNA damage-inducible protein GADD153
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human DDIT3. Species crossreactivity: mouse
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
21.3kDa (189aa), confirmed by MALDI-TOF.
OMIM
126337
3D Structure
ModBase 3D Structure for P35638
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 120-149 in the C-terminal region of human DDIT3. Species sequence homology: bovine, hamster
Conjugate
MaxLight405
Note
Preservative Free

Product Categories/Family for anti-DDIT3 antibody

Antibodies; Transcription Factors
Applications Tested/Suitable for anti-DDIT3 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF), FLISA
Application Notes for anti-DDIT3 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-DDIT3 antibody

Western Blot analysis in human skeletal muscle lysate (20ug/lane) using MBS6010216 (1:2000). Secondary IgG (HRP) Goat Anti-Rabbit at 1:10000 dilution. Predicted band size: 19kD Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-DDIT3 Polyclonal Antibody – Cat# MBS6291271

Western Blot (WB) of anti-DDIT3 antibody

Western Blot analysis in PC-3 whole cell lysate (20ug/lane) using MBS6010216 (1:2000). Secondary IgG (HRP) Goat Anti-Rabbit at 1:10000 dilution. Predicted band size: 19kD Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-DDIT3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6291271

Western Blot (WB) of anti-DDIT3 antibody

Western Blot analysis in PC-3 whole cell lysate (20ug/lane) using MBS6010216 (1:2000). Secondary IgG (HRP) Goat Anti-Rabbit at 1:10000 dilution. Predicted band size: 19kD Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-DDIT3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6291271

Immunofluorescence (IF) of anti-DDIT3 antibody

Fluorescent image of Hela cells stained withMBS6010216. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MBS6010216 (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). DDIT3 immunoreactivity is localized to Cytoplasm significantly.
Rabbit Anti-DDIT3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6291271

Flow Cytometry (FC/FACS) of anti-DDIT3 antibody

Flow Cytometric analysis of 293 cells using MBS6010216 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-DDIT3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6291271

Product References and Citations for anti-DDIT3 antibody

Park, S.H., et al. J. Immunol. 185(9):5522-5530(2010). Goodall, J.C., et al. Proc. Natl. Acad. Sci. U.S.A. 107(41):17698-17703(2010). Zhang, H.M., et al. J. Virol. 84(17):8446-8459(2010). Cazanave, S.C., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 299 (1), G236-G243 (2010): Wang, Y.L., et al. J. Exp. Clin. Cancer Res. 29, 54 (2010).

Other Notes

Small volumes of anti-DDIT3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DDIT3 purchase

MBS6291271 is an antibody targeting DDIT3. Also known as DDIT3, CT (A135) (DDIT3, CHOP, CHOP10, GADD153, DNA damage-inducible transcript 3 protein, C/EBP-homologous protein, C/EBP-homologous protein 10, Growth arrest and DNA damage-inducible protein GADD153) (MaxLight 405). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DDIT3. It may not necessarily be applicable to this product.

NCBI GI #
21361118
NCBI GeneID
1649
NCBI Accession #
NP_004074
UniProt Primary Accession #
P35638
UniProt Secondary Accession #
F8VS99
UniProt Related Accession #
P35638
GenBank Nucleotide #
NM_004083.5
NCBI Official Full Name
DNA damage-inducible transcript 3 protein isoform 2
NCBI Official Synonym Full Names
DNA damage inducible transcript 3
NCBI Official Symbol
DDIT3
NCBI Official Synonym Symbols
CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; C/EBPzeta
UniProt Protein Name
DNA damage-inducible transcript 3 protein
UniProt Synonym Protein Names
C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153
Protein Family
DNA damage-inducible transcript 3 protein
UniProt Gene Name
DDIT3
UniProt Synonym Gene Names
CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10
UniProt Entry Name
DDIT3_HUMAN
NCBI Protein Information
DNA damage-inducible transcript 3 protein

UniProt Comments for DDIT3

CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.

Protein type: Autophagy; Transcription factor; DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 12q13.1-q13.2

Cellular Component: nucleoplasm; late endosome; nucleus; cytosol

Molecular Function: leucine zipper domain binding; protein binding; DNA binding; protein heterodimerization activity; transcription factor binding; transcription factor activity; transcription corepressor activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-8 production; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; cell cycle arrest; ER overload response; regulation of transcription in response to stress; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; unfolded protein response; negative regulation of transcription factor activity; blood vessel maturation; mRNA transcription from RNA polymerase II promoter; response to unfolded protein; response to starvation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; cell redox homeostasis; release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; inhibition of CREB transcription factor; negative regulation of myoblast differentiation; response to DNA damage stimulus

Disease: Myxoid Liposarcoma
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Research Articles on DDIT3

1. asthmatic patients exhibited aberrant Chop expression along with endoplasmic reticulum stress

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DDIT3 antibody

 Reference Product  PubMed Publications
 ATF4 antibody  >273 publications with DDIT3 and ATF4
 JUN antibody  >266 publications with DDIT3 and JUN
 ATF6 antibody  >202 publications with DDIT3 and ATF6
 XBP1 antibody  >148 publications with DDIT3 and XBP1
 ATF3 antibody  >62 publications with DDIT3 and ATF3
 BCL2 antibody  >27 publications with DDIT3 and BCL2
 CEBPB antibody  >11 publications with DDIT3 and CEBPB
 JDP2 antibody  >2 publications with DDIT3 and JDP2
 MAPK14 antibody  >1 publications with DDIT3 and MAPK14

Pathways associated with anti-DDIT3 antibody

 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 ATF4 Activates Genes Pathway antibodies  ATF4 Activates Genes Pathway Diagram
 ATF6 (ATF6-alpha) Activates Chaperone Genes Pathway antibodies  ATF6 (ATF6-alpha) Activates Chaperone Genes Pathway Diagram
 ATF6 (ATF6-alpha) Activates Chaperones Pathway antibodies  ATF6 (ATF6-alpha) Activates Chaperones Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram

Diseases associated with anti-DDIT3 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >173 publications with DDIT3 and Nervous System Diseases
 Disease Models, Animal Antibodies  >158 publications with DDIT3 and Disease Models, Animal
 Necrosis Antibodies  >145 publications with DDIT3 and Necrosis
 Inflammation Antibodies  >132 publications with DDIT3 and Inflammation
 Brain Diseases Antibodies  >124 publications with DDIT3 and Brain Diseases
 Liposarcoma, Myxoid Antibodies  >118 publications with DDIT3 and Liposarcoma, Myxoid
 Liver Diseases Antibodies  >117 publications with DDIT3 and Liver Diseases
 Liver Neoplasms Antibodies  >63 publications with DDIT3 and Liver Neoplasms
 Breast Neoplasms Antibodies  >57 publications with DDIT3 and Breast Neoplasms
 Carcinoma, Hepatocellular Antibodies  >56 publications with DDIT3 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-DDIT3 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >200 publications with DDIT3 and Brain
 Liver Antibodies  >189 publications with DDIT3 and Liver
 Blood Antibodies  >179 publications with DDIT3 and Blood
 Muscle Antibodies  >125 publications with DDIT3 and Muscle
 Lung Antibodies  >107 publications with DDIT3 and Lung
 Kidney Antibodies  >105 publications with DDIT3 and Kidney
 Pancreas Antibodies  >102 publications with DDIT3 and Pancreas
 Vascular Antibodies  >94 publications with DDIT3 and Vascular
 Heart Antibodies  >89 publications with DDIT3 and Heart
 Bone Antibodies  >62 publications with DDIT3 and Bone
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