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DEPDC5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9459407

As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs,… More details

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Product Overview

Online Datasheet in PDF
Product Name
DEPDC5, Polyclonal Antibody
Full Product Name
DEPDC5 (Phospho-Ser993) Antibody
Product Gene Name
Product Synonym Names
GATOR complex protein DEPDC5; DEP domain-containing protein 5; KIAA0645
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Rabbit IgG in phosphate buffered saline (without M
Purity/Purification
The antibody was purified from rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Concentration
1mg/ml (lot specific)
Specificity
DEPDC5 (Phospho-Ser993) Antibody detects endogenous levels of DEPDC5 only when phosphorylated at Ser993.
Preparation and Storage
Store at -20 degree C for 1 year
Molecular Weight
177,910 Da
OMIM
604364
3D Structure
ModBase 3D Structure for O75140
Immunogen
A synthesized peptide derived from human DEPDC5 around the phosphorylation site of Ser993.

Product Categories/Family for anti-DEPDC5 antibody

Applications Tested/Suitable for anti-DEPDC5 antibody
Western Blot (WB)
Application Notes for anti-DEPDC5 antibody
WB: 1/500-1/2000

Other Notes

Small volumes of anti-DEPDC5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DEPDC5 purchase

MBS9459407 is an antibody targeting DEPDC5. Also known as DEPDC5 (Phospho-Ser993) Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DEPDC5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
DEP domain-containing protein 5
NCBI Official Synonym Full Names
DEP domain containing 5
NCBI Official Symbol
DEPDC5
NCBI Official Synonym Symbols
DEP.5; FFEVF
UniProt Protein Name
DEP domain-containing protein 5
UniProt Gene Name
DEPDC5
UniProt Synonym Gene Names
KIAA0645
UniProt Entry Name
DEPD5_HUMAN
NCBI Protein Information
DEP domain-containing protein 5

NCBI Summary for DEPDC5

This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
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UniProt Comments for DEPDC5

DEPDC5: Belongs to the IML1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: cytosol; lysosomal membrane; lysosome; perinuclear region of cytoplasm

Molecular Function: GTPase activator activity; protein complex binding

Biological Process: cellular response to amino acid starvation; negative regulation of TOR signaling pathway; positive regulation of GTPase activity

Disease: Epilepsy, Familial Focal, With Variable Foci
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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