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DGKD Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6472415

MBS6472415 is an antibody targeting DGKD. Also known as DGKD, CT (Diacylglycerol Kinase delta, Diglyceride Kinase delta, DGK-delta, DAG Kinase delta, 130kD Diacylglycerol Kinase, KIAA0145) (PE). Reactive in Human, Mouse. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DGKD, Polyclonal Antibody
Full Product Name
DGKD, CT (Diacylglycerol Kinase delta, Diglyceride Kinase delta, DGK-delta, DAG Kinase delta, 130kD Diacylglycerol Kinase, KIAA0145) (PE)
Product Gene Name
anti-DGKD antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein G Affinity Chromatography.
Specificity
Recognizes human DGKD. Species Crossreactivity: mouse.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
134,525 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (234263153..234380743). Location: 2q37.1
OMIM
601826
3D Structure
ModBase 3D Structure for Q16760
Immunogen
Synthetic peptide selected from the C-terminal region of human DGKD (KLH).
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-DGKD antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-DGKD antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-DGKD antibody
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-DGKD antibody

Western blot analysis of anti-anti-DGK delta Pab in Jurkat cell line lysates (35ug/lane). DGK delta (arrow) was detected using the purified Pab.
Rabbit Anti-DGKD Polyclonal Antibody – Cat# MBS6472415

Immunohistochemistry (IHC) of anti-DGKD antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-DGKD Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6472415

Product References and Citations for anti-DGKD antibody

1. Sakane, F., et al., J. Biol. Chem. 271(14):8394-8401 (1996). 2. Nagase, T., et al., DNA Res. 2(4):167-174 (1995).

Other Notes

Small volumes of anti-DGKD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DGKD. It may not necessarily be applicable to this product.

NCBI GI #
25777596
NCBI GeneID
8527
NCBI Accession #
NP_003639.2
UniProt Primary Accession #
Q16760
UniProt Secondary Accession #
Q14158; Q6PK55; Q8NG53
UniProt Related Accession #
Q16760
GenBank Nucleotide #
NM_003648.2
NCBI Official Full Name
diacylglycerol kinase delta isoform 1
NCBI Official Synonym Full Names
diacylglycerol kinase, delta 130kDa
NCBI Official Symbol
DGKD
NCBI Official Synonym Symbols
dgkd-2; DGKdelta
UniProt Protein Name
Diacylglycerol kinase delta
UniProt Synonym Protein Names
130 kDa diacylglycerol kinase; Diglyceride kinase delta
Protein Family
Diacylglycerol kinase
UniProt Gene Name
DGKD
UniProt Synonym Gene Names
KIAA0145; DAG kinase delta; DGK-delta
UniProt Entry Name
DGKD_HUMAN
NCBI Protein Information
diacylglycerol kinase delta; DGK-delta; DAG kinase delta; diglyceride kinase delta

NCBI Summary for DGKD

This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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UniProt Comments for DGKD

DGKD: May function as signaling molecule. Belongs to the eukaryotic diacylglycerol kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerophospholipid; Lipid Metabolism - glycerolipid; Kinase, lipid; EC 2.7.1.107; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane

Molecular Function: diacylglycerol binding; protein binding; protein homodimerization activity; protein heterodimerization activity; metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; second-messenger-mediated signaling; platelet activation; multicellular organismal development; endocytosis; signal transduction; response to organic substance; protein transport; protein kinase C activation; diacylglycerol metabolic process; blood coagulation; cell growth; phosphorylation; protein homooligomerization
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Research Articles on DGKD

1. Diacylglycerol signaling limits Akt activation through diacylglycerol kinase delta and PHLPP2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DGKD antibody

 Reference Product  PubMed Publications
 PRKCA antibody  >1 publications with DGKD and PRKCA
 IGSF21 antibody  >1 publications with DGKD and IGSF21

Pathways associated with anti-DGKD antibody

 Products by Pathway  Pathway Diagram
 Effects Of PIP2 Hydrolysis Pathway antibodies  Effects Of PIP2 Hydrolysis Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Phosphatidylinositol Signaling System Pathway antibodies  Phosphatidylinositol Signaling System Pathway Diagram

Diseases associated with anti-DGKD antibody

 Disease Name  Pubmed Publications
 Diabetes Mellitus, Type 2 Antibodies  >2 publications with DGKD and Diabetes Mellitus, Type 2
 Hyperglycemia Antibodies  >1 publications with DGKD and Hyperglycemia
 Lung Neoplasms Antibodies  >1 publications with DGKD and Lung Neoplasms
 Congenital Abnormalities Antibodies  >1 publications with DGKD and Congenital Abnormalities

Organs/Tissues associated with anti-DGKD antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >2 publications with DGKD and Muscle
 Prostate Antibodies  >1 publications with DGKD and Prostate
 Lung Antibodies  >1 publications with DGKD and Lung
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