• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

DGKE Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9128760
SPECIAL PROMOTION: Get FREE $5 Amazon gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for… More details

$225 / 0.02 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DGKE, Polyclonal Antibody
Full Product Name
DGKE Polyclonal Antibody
Product Gene Name
anti-DGKE antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
64kDa
OMIM
601440
3D Structure
ModBase 3D Structure for P52429
Immunogen
Recombinant protein of human DGKE
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-DGKE antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-DGKE antibody
WB 1:500 - 1:2000
IHC 1:50 - 1:200

Western Blot (WB) of anti-DGKE antibody

Western blot analysis of extracts of various cell lines, using DGKE antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Rabbit Anti-DGKE Polyclonal Antibody – Cat# MBS9128760

Immunohistochemistry (IHC) of anti-DGKE antibody

Immunohistochemistry of paraffin-embedded human gastric using DGKE antibody at dilution of 1:100 (40x lens).
Rabbit Anti-DGKE Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9128760

Immunohistochemistry (IHC) of anti-DGKE antibody

Immunohistochemistry of paraffin-embedded mouse kidney using DGKE antibody at dilution of 1:100 (40x lens).
Rabbit Anti-DGKE Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9128760

Other Notes

Small volumes of anti-DGKE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DGKE purchase

MBS9128760 is an antibody targeting DGKE. Also known as DGKE Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DGKE. It may not necessarily be applicable to this product.

NCBI GI #
4503313
NCBI GeneID
8526
NCBI Accession #
NP_003638.1
UniProt Primary Accession #
P52429
UniProt Secondary Accession #
Q8TBM4; Q9UKQ3
UniProt Related Accession #
P52429
GenBank Nucleotide #
NM_003647.2
NCBI Official Full Name
diacylglycerol kinase epsilon
NCBI Official Synonym Full Names
diacylglycerol kinase, epsilon 64kDa
NCBI Official Symbol
DGKE
NCBI Official Synonym Symbols
DGK; DAGK5; DAGK6; NPHS7
UniProt Protein Name
Diacylglycerol kinase epsilon
UniProt Synonym Protein Names
Diglyceride kinase epsilon; DGK-epsilon
Protein Family
Diacylglycerol kinase
UniProt Gene Name
DGKE
UniProt Synonym Gene Names
DAGK5; DAG kinase epsilon; DGK-epsilon
UniProt Entry Name
DGKE_HUMAN
NCBI Protein Information
diacylglycerol kinase epsilon

UniProt Comments for DGKE

DGKE: Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition. Belongs to the eukaryotic diacylglycerol kinase family.

Protein type: Membrane protein, multi-pass; Motility/polarity/chemotaxis; Membrane protein, integral; Lipid Metabolism - glycerolipid; Lipid Metabolism - glycerophospholipid; EC 2.7.1.107; Kinase, lipid

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: platelet activation; protein kinase C activation; blood coagulation; phosphorylation; phospholipid biosynthetic process

Disease: Nephrotic Syndrome, Type 7
Show more

Research Articles on DGKE

1. Data suggest that complement dysregulation influences the onset and disease severity in carriers of diacylglycerol kinase-epsilon mutations

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DGKE antibody

 Reference Product  PubMed Publications
 PLCD3 antibody  >1 publications with DGKE and PLCD3

Pathways associated with anti-DGKE antibody

 Products by Pathway  Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Effects Of PIP2 Hydrolysis Pathway antibodies  Effects Of PIP2 Hydrolysis Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram

Diseases associated with anti-DGKE antibody

 Disease Name  Pubmed Publications
 Atypical Hemolytic Uremic Syndrome Antibodies  >11 publications with DGKE and Atypical Hemolytic Uremic Syndrome
 Kidney Diseases Antibodies  >8 publications with DGKE and Kidney Diseases
 Liver Diseases Antibodies  >1 publications with DGKE and Liver Diseases
 Depressive Disorder Antibodies  >1 publications with DGKE and Depressive Disorder

Organs/Tissues associated with anti-DGKE antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >5 publications with DGKE and Kidney
 Liver Antibodies  >2 publications with DGKE and Liver
 Brain Antibodies  >2 publications with DGKE and Brain
 Vascular Antibodies  >1 publications with DGKE and Vascular
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000