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EDC3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904222

Enhancer of mRNA-decapping protein 3 (EDC3) binds single-stranded RNA. In the process of mRNA degradation, EDC3 may play a role in mRNA decapping. It may play a role in spermiogenesis and oogenesis [taken from the Universal Protein Resource (UniProt)… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
EDC3, Polyclonal Antibody
Full Product Name
Rabbit anti-EDC3 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
MRT50; YjeF_N2; YJEFN2; yjeF N-terminal domain-containing protein 2; yjeF domain-containing protein 1; yjeF domain containing; YJDC; LSM16 homolog; LSM16; hYjeF_N2-15q23; hYjeF_N2; enhancer of mRNA decapping 3 homolog; enhancer of mRNA-decapping protein 3; LSM16 homolog (EDC3, S. cerevisiae); Enhancer of mRNA-decapping protein 3; enhancer of mRNA decapping 3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
56,078 Da
OMIM
609842
3D Structure
ModBase 3D Structure for Q96F86
Predicted Reactivity
Orangutan
Immunogen
Between 50 and 100
Conjugation
Unconjugated

Product Categories/Family for anti-EDC3 antibody

Applications Tested/Suitable for anti-EDC3 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-EDC3 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-EDC3 antibody

Detection of human EDC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-EDC3 antibody MBS3904222 (lot MBS3904222-1) used for IP at 6 ug/mg lysate. EDC3 was also immunoprecipitated by rabbit anti-EDC3 antibody BL13994. For blotting immunoprecipitated EDC3, MBS3904222 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-EDC3 Polyclonal Antibody – Cat# MBS3904222

Western Blot (WB) of anti-EDC3 antibody

Detection of human and mouse EDC3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3cells. Antibodies: Affinity purified rabbit anti-EDC3 antibody MBS3904222 (lot MBS3904222-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-EDC3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904222

Other Notes

Small volumes of anti-EDC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EDC3 purchase

MBS3904222 is an antibody targeting EDC3. Also known as Rabbit anti-EDC3 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EDC3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
enhancer of mRNA-decapping protein 3
NCBI Official Synonym Full Names
enhancer of mRNA decapping 3
NCBI Official Symbol
EDC3
NCBI Official Synonym Symbols
YJDC; LSM16; MRT50; YJEFN2
UniProt Protein Name
Enhancer of mRNA-decapping protein 3
UniProt Synonym Protein Names
LSM16 homolog; YjeF N-terminal domain-containing protein 2; YjeF_N2; hYjeF_N2; YjeF domain-containing protein 1
UniProt Gene Name
EDC3
UniProt Synonym Gene Names
LSM16; YJDC; YJEFN2; YjeF_N2; hYjeF_N2
UniProt Entry Name
EDC3_HUMAN
NCBI Protein Information
enhancer of mRNA-decapping protein 3

NCBI Summary for EDC3

EDC3 is associated with an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end (Fenger-Gron et al., 2005 [PubMed 16364915]).[supplied by OMIM, Mar 2008]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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