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EIF2AK2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6475195

MBS6475195 is an antibody targeting EIF2AK2. Also known as EIF2AK2, CT (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2, eIF-2A Protein Kinase 2, Interferon-induced, Double-stranded RNA-activated Protein Kinase, Interferon-inducible RNA-dependent… More details

$1060 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EIF2AK2, Polyclonal Antibody
Full Product Name
EIF2AK2, CT (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2, eIF-2A Protein Kinase 2, Interferon-induced, Double-stranded RNA-activated Protein Kinase, Interferon-inducible RNA-dependent pRotein Kinase, p68 kinase, Protein Kinase RNA-activated
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human PRKR. Species Crossreactivity: mouse.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
62kDa
3D Structure
ModBase 3D Structure for Q52M43
Immunogen
Synthetic peptide selected from the C-terminal region of human PRKR (KLH).
Conjugate
MaxLight650
Note
Preservative Free
Applications Tested/Suitable for anti-EIF2AK2 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-EIF2AK2 antibody
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-EIF2AK2 antibody

The anti-PRKR Pab is used in Western blot to detect PRKR in mouse uterus tissue lysate (Lane 1) and HepG2 cell lysate (Lane 2).
Rabbit Anti-EIF2AK2 Polyclonal Antibody – Cat# MBS6475195

Western Blot (WB) of anti-EIF2AK2 antibody

Western blot analysis of EIF2AK2 (arrow) using PRKR Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF2AK2 gene (Lane 2) (Origene Technologies).
Rabbit Anti-EIF2AK2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6475195

Immunohistochemistry (IHC) of anti-EIF2AK2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-EIF2AK2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6475195

Product References and Citations for anti-EIF2AK2 antibody

1. Peel, A.L., et al., Neurobiol. Dis. 14(1):52-62 (2003). 2. Pang, Q., et al., J. Biol. Chem. 277(51):49638-49643 (2002). 3. Kim, S.H., et al., Oncogene 21(57):8741-8748 (2002). 4. Takizawa, T., et al., Eur. J. Biochem. 269(24):6126-6132 (2002). 5. Peters, G.A., et al., J. Virol. 76(21):11054-11064 (2002).

Other Notes

Small volumes of anti-EIF2AK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF2AK2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interferon-induced, double-stranded RNA-activated protein kinase isoform a
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2 alpha kinase 2
NCBI Official Symbol
EIF2AK2
NCBI Official Synonym Symbols
PKR; PRKR; EIF2AK1; PPP1R83
NCBI Protein Information
interferon-induced, double-stranded RNA-activated protein kinase

NCBI Summary for EIF2AK2

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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