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eIF4B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9502587

MBS9502587 is an antibody targeting eIF4B. Also known as eIF4B (Phospho-Ser422) Antibody. Reactive in Human, Mouse. Host: Rabbit. More details

$300 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
eIF4B, Polyclonal Antibody
Full Product Name
eIF4B (Phospho-Ser422) Antibody
Product Gene Name
Product Synonym Names
Eukaryotic translation initiation factor 4B; IF4B; eIF-4B
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Concentration
1 mg/ml (lot specific)
Specificity
eIF4B (Phospho-Ser422) Antibody detects endogenous levels of eIF4B only when phosphorylated at Ser422.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Molecular Weight
69 kDa
OMIM
X55733 Genomic DNA
3D Structure
ModBase 3D Structure for P23588
Target Modification
Phospho
Modification Sites
H:S422; M:S422
Immunogen
The antiserum was produced against synthesized peptide derived from human eIF4B around the phosphorylation site of Ser422.
Immunogen Range
388-437
Applications Tested/Suitable for anti-eIF4B antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-eIF4B antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:40000

Immunofluorescence (IF) of anti-eIF4B antibody

Immunofluorescence analysis of NIH/3T3 cells, using eIF4B (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.
Rabbit Anti-eIF4B Polyclonal Antibody – Cat# MBS9502587

Immunohistochemistry (IHC) of anti-eIF4B antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using eIF4B (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.
Rabbit Anti-eIF4B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9502587

Western Blot (WB) of anti-eIF4B antibody

Western blot analysis of lysates from NIH/3T3 cells, using eIF4B (Phospho-Ser422) Antibody. The lane on the right is blocked with the phospho peptide.
Rabbit Anti-eIF4B Polyclonal Antibody, Western Blot (WB) – Cat# MBS9502587

Other Notes

Small volumes of anti-eIF4B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for eIF4B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
eukaryotic translation initiation factor 4B isoform 3
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4B
NCBI Official Symbol
EIF4B
NCBI Official Synonym Symbols
EIF-4B; PRO1843
UniProt Protein Name
Eukaryotic translation initiation factor 4B
UniProt Gene Name
EIF4B
UniProt Synonym Gene Names
eIF-4B
NCBI Protein Information
eukaryotic translation initiation factor 4B

UniProt Comments for eIF4B

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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