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ELF5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6473705

MBS6473705 is an antibody targeting ELF5. Also known as ELF5, ID (ETS-related Transcription Factor Elf-5, E74-like Factor 5, Epithelium-specific Ets Transcription Factor 2, ESE-2, Epithelium-restricted ESE-1-related Ets Factor, ESE2) (PE). Reactive in Human,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ELF5, Polyclonal Antibody
Full Product Name
ELF5, ID (ETS-related Transcription Factor Elf-5, E74-like Factor 5, Epithelium-specific Ets Transcription Factor 2, ESE-2, Epithelium-restricted ESE-1-related Ets Factor, ESE2) (PE)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human ELF5. Species Crossreactivity: mouse.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
29kDa
OMIM
605169
3D Structure
ModBase 3D Structure for Q9UKW6
Immunogen
Synthetic peptide selected from the center region of human ELF5 (KLH).
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Applications Tested/Suitable for anti-ELF5 antibody
Western Blot (WB), FLISA
Application Notes for anti-ELF5 antibody
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ELF5 antibody

Western blot analysis of ELF5 Antibody (Center) in MCF7(lane 1),T47D cell line lysates(lane 2) and mouse lung tissues(lane 3) lysates(35ug/lane). ELF5 (arrow) was detected using the purified Pab.
Rabbit Anti-ELF5 Polyclonal Antibody – Cat# MBS6473705

Western Blot (WB) of anti-ELF5 antibody

Western blot analysis of ELF5 (arrow) using rabbit polyclonal ELF5 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ELF5 gene.
Rabbit Anti-ELF5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6473705

Product References and Citations for anti-ELF5 antibody

1. Choi, Y.S., Biochem. J. 398(3), 497-507 (2006). 2. Tummala, R., J. Cell. Biochem. 97(4), 766-781 (2006).

Other Notes

Small volumes of anti-ELF5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ELF5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ETS-related transcription factor Elf-5 isoform 1
NCBI Official Synonym Full Names
E74 like ETS transcription factor 5
NCBI Official Symbol
ELF5
NCBI Official Synonym Symbols
ESE2
UniProt Protein Name
ETS-related transcription factor Elf-5
UniProt Synonym Protein Names
E74-like factor 5; Epithelium-restricted ESE-1-related Ets factor; Epithelium-specific Ets transcription factor 2
UniProt Gene Name
ELF5
UniProt Synonym Gene Names
ESE2; ESE-2
UniProt Entry Name
ELF5_HUMAN
NCBI Protein Information
ETS-related transcription factor Elf-5

NCBI Summary for ELF5

The protein encoded by this gene is a member of an epithelium-specific subclass of the Ets transcritpion factor family. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]
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UniProt Comments for ELF5

ELF5: Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation. Belongs to the ETS family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 11p13-p12

Cellular Component: cytoplasm; nucleus

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; cell proliferation; ectoderm development; positive regulation of transcription from RNA polymerase II promoter; cell differentiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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