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ER81 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9604655

Function: Transcriptional activator that binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3'.Post-translational Modifications: Sumoylated. Phosphorylated at Ser-191 and Ser-216 by RPS6KA1 and RPS6KA5; phosphorylation activates… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ER81, Polyclonal Antibody
Full Product Name
ER81 Antibody
Product Gene Name
anti-ER81 antibody
Product Synonym Gene Name
ETV1
Product Synonym Names
DKFZp781L0674; ER81; ER81 protein; ER81, mouse, homolog of; ETS translocation variant 1; ets variant gene 1; Ets-related protein 81; Etsrp81; ETV 1; ETV1; ETV1_HUMAN; MGC104699; MGC120533; MGC120534
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
ER81 antibody detects endogenous levels of total ER81
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
ER81 peptide (MBS9623372) is used for blocking the activity of ER81 antibody (MBS9604655)
Molecular Weight
Observed: 60 kD
Predicted: 56 kDa
3D Structure
ModBase 3D Structure for P50549
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Tissue Specificity
Very highly expressed in brain, highly expressed in testis, lung and heart, moderately in spleen, small intestine, pancreas and colon, weakly in liver, prostate and thymus, very weakly in skeletal muscle, kidney and ovary and not in placenta and peripheral blood leukocytes.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (91%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ER81 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-ER81 antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-ER81 antibody

MBS9604655 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-ER81 Polyclonal Antibody – Cat# MBS9604655

Western Blot (WB) of anti-ER81 antibody

Western blot analysis of extracts from HUVEC cells treated with PMA using ER81 antibody.The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-ER81 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9604655

Other Notes

Small volumes of anti-ER81 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ER81 purchase

MBS9604655 is an antibody targeting ER81. Also known as ER81 Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ER81. It may not necessarily be applicable to this product.

NCBI GI #
253683427
NCBI GeneID
2115
NCBI Accession #
NP_001156619.1
UniProt Primary Accession #
P50549
UniProt Secondary Accession #
O75849; Q4KMQ6; Q59GA7; A4D118; B2R768; B7Z2I4; B7Z618; B7Z9P2; C9JT37; E9PHB1; F5GXR2
UniProt Related Accession #
P50549
GenBank Nucleotide #
NM_001163147.1
NCBI Official Full Name
ETS translocation variant 1 isoform b
NCBI Official Synonym Full Names
ETS variant 1
NCBI Official Symbol
ETV1
NCBI Official Synonym Symbols
ER81
UniProt Protein Name
ETS translocation variant 1
UniProt Synonym Protein Names
Ets-related protein 81
UniProt Gene Name
ETV1
UniProt Synonym Gene Names
ER81
NCBI Protein Information
ETS translocation variant 1

NCBI Summary for ER81

This gene encodes a member of the ETS (E twenty-six) family of transcription factors. The ETS proteins regulate many target genes that modulate biological processes like cell growth, angiogenesis, migration, proliferation and differentiation. All ETS proteins contain an ETS DNA-binding domain that binds to DNA sequences containing the consensus 5'-CGGA[AT]-3'. The protein encoded by this gene contains a conserved short acidic transactivation domain (TAD) in the N-terminal region, in addition to the ETS DNA-binding domain in the C-terminal region. This gene is involved in chromosomal translocations, which result in multiple fusion proteins including EWS-ETV1 in Ewing sarcoma and at least 10 ETV1 partners (see PMID: 19657377, Table 1) in prostate cancer. In addition to chromosomal rearrangement, this gene is overexpressed in prostate cancer, melanoma and gastrointestinal stromal tumor. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2016]
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UniProt Comments for ER81

Transcriptional activator that binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3'.

Research Articles on ER81

1. Our data indicate that miR-17-5p acts as a tumour suppressor in Triple-negative breast cancer (TNBC)by targeting ETV1, and a low-abundance of miR-17-5p may be involved in the pathogenesis of TNBC. These findings indicate that miR-17-5p may be a therapeutic target for TNBC

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ER81 antibody

 Reference Product  PubMed Publications
 ERG antibody  >83 publications with ER81 and ERG
 RPS6KA1 antibody  >2 publications with ER81 and RPS6KA1
 MAPKAPK2 antibody  >2 publications with ER81 and MAPKAPK2
 EP300 antibody  >1 publications with ER81 and EP300

Pathways associated with anti-ER81 antibody

 Products by Pathway  Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 P38 Signaling Mediated By MAPKAP Kinases Pathway antibodies  P38 Signaling Mediated By MAPKAP Kinases Pathway Diagram

Diseases associated with anti-ER81 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >121 publications with ER81 and Neoplasms
 Prostatic Diseases Antibodies  >61 publications with ER81 and Prostatic Diseases
 Genital Diseases, Male Antibodies  >61 publications with ER81 and Genital Diseases, Male
 Prostatic Neoplasms Antibodies  >61 publications with ER81 and Prostatic Neoplasms
 Adenocarcinoma Antibodies  >15 publications with ER81 and Adenocarcinoma
 Breast Neoplasms Antibodies  >13 publications with ER81 and Breast Neoplasms
 Hyperplasia Antibodies  >8 publications with ER81 and Hyperplasia
 Nervous System Diseases Antibodies  >8 publications with ER81 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >4 publications with ER81 and Neoplasms, Experimental
 Aneuploidy Antibodies  >3 publications with ER81 and Aneuploidy

Organs/Tissues associated with anti-ER81 antibody

 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >74 publications with ER81 and Prostate
 Brain Antibodies  >35 publications with ER81 and Brain
 Bone Antibodies  >19 publications with ER81 and Bone
 Muscle Antibodies  >11 publications with ER81 and Muscle
 Embryonic Tissue Antibodies  >10 publications with ER81 and Embryonic Tissue
 Ganglia Antibodies  >8 publications with ER81 and Ganglia
 Vascular Antibodies  >4 publications with ER81 and Vascular
 Mammary Gland Antibodies  >4 publications with ER81 and Mammary Gland
 Lung Antibodies  >4 publications with ER81 and Lung
 Heart Antibodies  >3 publications with ER81 and Heart
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