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GPA33 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9608518

Function: May play a role in cell-cell recognition and signaling.Post-translational Modifications: N-glycosylated, contains approximately 8 kDa of N-linked carbohydrate. Palmitoylated. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GPA33, Polyclonal Antibody
Full Product Name
GPA33 Antibody
Product Gene Name
Product Synonym Names
A33; Cell surface A33 antigen; Cell surface A33 antigen precursor; Glycoprotein A33 (transmembrane); Glycoprotein A33; Gpa33; GPA33_HUMAN; MGC129986; MGC129987; OTTHUMP00000032346; Transmembrane glycoprotein A33
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
GPA33 antibody detects endogenous levels of total GPA33
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
GPA33 peptide (MBS9627230) is used for blocking the activity of GPA33 antibody (MBS9608518)
Molecular Weight
Observed: 36 kDa
Predicted: 36 kDa
3D Structure
ModBase 3D Structure for Q99795
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human GPA33
Subcellular Location
Membrane.
Tissue Specificity
Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (91%), Bovine (91%), Horse (82%), Sheep (91%), Rabbit (91%), Dog (91%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-GPA33 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-GPA33 antibody
WB: 1:1000-3000
IHC: 1:200

Immunohistochemistry (IHC) of anti-GPA33 antibody

MBS9608518 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-GPA33 Polyclonal Antibody – Cat# MBS9608518

Western Blot (WB) of anti-GPA33 antibody

Western blot analysis GPA33 using HT29 whole cell lysates
Rabbit Anti-GPA33 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9608518

Other Notes

Small volumes of anti-GPA33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPA33 purchase

MBS9608518 is an antibody targeting GPA33. Also known as GPA33 Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GPA33. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cell surface A33 antigen
NCBI Official Synonym Full Names
glycoprotein A33
NCBI Official Symbol
GPA33
NCBI Official Synonym Symbols
A33
UniProt Protein Name
Cell surface A33 antigen
UniProt Synonym Protein Names
Glycoprotein A33
UniProt Gene Name
GPA33
NCBI Protein Information
cell surface A33 antigen

NCBI Summary for GPA33

The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily. [provided by RefSeq, Jul 2008]
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UniProt Comments for GPA33

May play a role in cell-cell recognition and signaling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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