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HADHA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6479390

MBS6479390 is an antibody targeting HADHA. Also known as HADHA, CT (HADH, ECHA, 78kD Gastrin-binding Protein, GBP, Long Chain Enoyl-CoA Hydratase, LCEH, Long Chain 3-hydroxyacyl-CoA Dehydrogenase, LCHAD, MGC1728, MTPA, Trifunctional Enzyme Subunit alpha… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HADHA, Polyclonal Antibody
Full Product Name
HADHA, CT (HADH, ECHA, 78kD Gastrin-binding Protein, GBP, Long Chain Enoyl-CoA Hydratase, LCEH, Long Chain 3-hydroxyacyl-CoA Dehydrogenase, LCHAD, MGC1728, MTPA, Trifunctional Enzyme Subunit alpha Mitochondrial, TP-alpha) (Azide free) (HRP)
Product Gene Name
anti-HADHA antibody
Product Synonym Names
Hydroxyacyl-CoA Dehydrogenase/3-ketoacyl-CoA Thiolase/enoyl-CoA Hydratase (Trifunctional Protein); alpha Subunit; EC=4.2.1.17; EC=1.1.1.211
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human HADHA.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
28,367 Da
OMIM
600890
3D Structure
ModBase 3D Structure for P40939
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues located somewhere between amino acids 737-763 of human HADHA, UniProt Accession # P40939.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-HADHA antibody

Antibodies; Binding Proteins
Applications Tested/Suitable for anti-HADHA antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-HADHA antibody
FC/FACS: 1:10-1:50
ELISA: 1:1,000
WB: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-HADHA antibody

Western blot analysis of HADHA in A2058 (lane 1), A375 (lane 2), NCI-H460 (lane 3) and Ramos (lane 4) cell line lysates (35ug/lane) usingMBS627977.
Rabbit Anti-HADHA Polyclonal Antibody – Cat# MBS6479390

Immunohistochemistry (IHC) of anti-HADHA antibody

IHC staining of formalin-fixed and paraffin-embedded human lung carcinoma usingMBS627977. HRP-conjugated secondary antibody and DAB staining were used to visualize the antigen.
Rabbit Anti-HADHA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6479390

Flow Cytometry (FC/FACS) of anti-HADHA antibody

Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibody was used for the analysis.
Rabbit Anti-HADHA Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6479390

Product References and Citations for anti-HADHA antibody

1. Sims, H.F., et.al., Proc. Natl. Acad. Sci. USA 92(3), 841-845 (1995).

Other Notes

Small volumes of anti-HADHA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HADHA. It may not necessarily be applicable to this product.

NCBI GI #
20127408
NCBI GeneID
3030
NCBI Accession #
NP_000173.2
UniProt Primary Accession #
P40939
UniProt Secondary Accession #
Q16679; Q53T69; Q53TA2; Q96GT7; Q9UQC5; B2R7L4; B4DYP2
UniProt Related Accession #
P40939
GenBank Nucleotide #
NM_000182.4
NCBI Official Full Name
trifunctional enzyme subunit alpha, mitochondrial
NCBI Official Synonym Full Names
hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit
NCBI Official Symbol
HADHA
NCBI Official Synonym Symbols
GBP; ECHA; HADH; LCEH; MTPA; LCHAD; TP-ALPHA
UniProt Protein Name
Trifunctional enzyme subunit alpha, mitochondrial
UniProt Synonym Protein Names
78 kDa gastrin-binding protein; TP-alphaIncluding the following 2 domains:Long-chain enoyl-CoA hydratase (EC:4.2.1.17); Long chain 3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.211)
Protein Family
Trifunctional enzyme
UniProt Gene Name
HADHA
UniProt Synonym Gene Names
HADH
UniProt Entry Name
ECHA_HUMAN
NCBI Protein Information
trifunctional enzyme subunit alpha, mitochondrial

NCBI Summary for HADHA

This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq, Jul 2008]
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Research Articles on HADHA

1. Mitochondrial Trifunctional-Protein depletion during hepatitis c virus infection rendered cells less responsive to alpha interferon treatment by impairing IFN-stimulated gene expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HADHA antibody

 Reference Product  PubMed Publications
 HADHB antibody  >16 publications with HADHA and HADHB
 ACADVL antibody  >2 publications with HADHA and ACADVL
 ACAA2 antibody  >2 publications with HADHA and ACAA2
 ACADL antibody  >1 publications with HADHA and ACADL

Pathways associated with anti-HADHA antibody

 Products by Pathway  Pathway Diagram
 Acyl Chain Remodeling Of CL Pathway antibodies  Acyl Chain Remodeling Of CL Pathway Diagram
 Beta Oxidation Of Decanoyl-CoA To Octanoyl-CoA-CoA Pathway antibodies  Beta Oxidation Of Decanoyl-CoA To Octanoyl-CoA-CoA Pathway Diagram
 Beta Oxidation Of Hexanoyl-CoA To Butanoyl-CoA Pathway antibodies  Beta Oxidation Of Hexanoyl-CoA To Butanoyl-CoA Pathway Diagram
 Beta Oxidation Of Lauroyl-CoA To Decanoyl-CoA-CoA Pathway antibodies  Beta Oxidation Of Lauroyl-CoA To Decanoyl-CoA-CoA Pathway Diagram
 Beta Oxidation Of Myristoyl-CoA To Lauroyl-CoA Pathway antibodies  Beta Oxidation Of Myristoyl-CoA To Lauroyl-CoA Pathway Diagram
 Beta Oxidation Of Octanoyl-CoA To Hexanoyl-CoA Pathway antibodies  Beta Oxidation Of Octanoyl-CoA To Hexanoyl-CoA Pathway Diagram
 Beta Oxidation Of Palmitoyl-CoA To Myristoyl-CoA Pathway antibodies  Beta Oxidation Of Palmitoyl-CoA To Myristoyl-CoA Pathway Diagram
 Biosynthesis Of Antibiotics Pathway antibodies  Biosynthesis Of Antibiotics Pathway Diagram
 Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies  Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
 Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies  Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram

Diseases associated with anti-HADHA antibody

 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >8 publications with HADHA and Fatty Liver
 LONG-CHAIN 3-HYDROXYACYL-CoA DEHYDROGENASE DEFICIENCY Antibodies  >7 publications with HADHA and LONG-CHAIN 3-HYDROXYACYL-CoA DEHYDROGENASE DEFICIENCY
 Liver Diseases Antibodies  >6 publications with HADHA and Liver Diseases
 Nervous System Diseases Antibodies  >3 publications with HADHA and Nervous System Diseases
 Heart Diseases Antibodies  >3 publications with HADHA and Heart Diseases
 Muscular Diseases Antibodies  >3 publications with HADHA and Muscular Diseases
 Cardiovascular Diseases Antibodies  >3 publications with HADHA and Cardiovascular Diseases
 Necrosis Antibodies  >2 publications with HADHA and Necrosis
 Trifunctional Protein Deficiency With Myopathy And Neuropathy Antibodies  >2 publications with HADHA and Trifunctional Protein Deficiency With Myopathy And Neuropathy
 Acute Kidney Injury Antibodies  >1 publications with HADHA and Acute Kidney Injury

Organs/Tissues associated with anti-HADHA antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >12 publications with HADHA and Liver
 Muscle Antibodies  >8 publications with HADHA and Muscle
 Blood Antibodies  >8 publications with HADHA and Blood
 Heart Antibodies  >5 publications with HADHA and Heart
 Skin Antibodies  >2 publications with HADHA and Skin
 Adipose Tissue Antibodies  >2 publications with HADHA and Adipose Tissue
 Thyroid Antibodies  >2 publications with HADHA and Thyroid
 Eye Antibodies  >1 publications with HADHA and Eye
 Placenta Antibodies  >1 publications with HADHA and Placenta
 Kidney Antibodies  >1 publications with HADHA and Kidney
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