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HECW2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4506735

MBS4506735 is an antibody targeting HECW2. Also known as Rabbit anti-HECW2 Antibody. Reactive in Human, Mouse. Host: Rabbit. More details

$250 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HECW2, Polyclonal Antibody
Full Product Name
Rabbit anti-HECW2 Antibody
Product Gene Name
Product Synonym Names
KIAA1301; NEDL2; E3 ubiquitin-protein ligase HECW2; HECT, C2 and WW domain-containing protein 2; NEDD4-like E3 ubiquitin-protein ligase 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze/thaw cycles.
Molecular Weight
175,769 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (197063977..197457335, complement). Location: 2q32.3
3D Structure
ModBase 3D Structure for Q9P2P5
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HECW2. The exact sequence is proprietary.

Product Categories/Family for anti-HECW2 antibody

ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-HECW2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunochemistry (IC)
Application Notes for anti-HECW2 antibody
WB: 1:500-1:1000
IF:IC: 1:100-1:500

Western Blot (WB) of anti-HECW2 antibody

Western blot analysis of HECW2 expression in A549 (A), NIH3T3 (B) whole cell lysates.
Rabbit Anti-HECW2 Polyclonal Antibody – Cat# MBS4506735

Immunofluorescence (IF) of anti-HECW2 antibody

Immunofluorescent analysis of HECW2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-HECW2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS4506735

Other Notes

Small volumes of anti-HECW2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HECW2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
E3 ubiquitin-protein ligase HECW2
NCBI Official Synonym Full Names
HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
NCBI Official Symbol
HECW2
NCBI Official Synonym Symbols
NEDL2
UniProt Protein Name
E3 ubiquitin-protein ligase HECW2
UniProt Synonym Protein Names
HECT, C2 and WW domain-containing protein 2; NEDD4-like E3 ubiquitin-protein ligase 2
UniProt Gene Name
HECW2
UniProt Synonym Gene Names
KIAA1301; NEDL2
UniProt Entry Name
HECW2_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase HECW2; NEDD4-related E3 ubiquitin ligase NEDL2; NEDD4-like E3 ubiquitin-protein ligase 2; HECT, C2 and WW domain-containing protein 2

UniProt Comments for HECW2

HECW2: E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 2q32.3

Cellular Component: cytoplasm; nucleus

Molecular Function: ubiquitin-protein ligase activity; ligase activity

Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-HECW2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1 publications with HECW2 and Brain
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