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HEXIM1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903408

Hexamethylene bis-acetamide-inducible protein 1 (HEXIM1) is a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA, HEXIM1sequesters P-TEFb in a large inactive 7SK snRNP complex… More details

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Product Overview

Online Datasheet in PDF
Product Name
HEXIM1, Polyclonal Antibody
Full Product Name
Rabbit anti-HEXIM1 Antibody, Affinity Purified
Product Gene Name
anti-HEXIM1 antibody
Product Synonym Names
cardiac lineage protein 1; hexamthylene bis-acetamide inducible 1; protein HEXIM1; menage a quatre protein 1; menage a quatre 1; MAQ1; HMBA-inducible; HIS1; hexamethylene-bis-acetamide-inducible transcript 1; hexamethylene bis-acetamide-inducible protein 1; hexamethylene bisacetamide-inducible protein; hexamethylene bis-acetamide inducible 1; estrogen down-regulated gene 1 protein; CLP1; EDG1; Protein HEXIM1; hexamethylene bisacetamide inducible 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
40,623 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (43224684..43229468). Location: 17q21.31
OMIM
607328
3D Structure
ModBase 3D Structure for O94992
Predicted Reactivity
Rat, Bovine
Immunogen
Between 200 and 250
Conjugation
Unconjugated

Product Categories/Family for anti-HEXIM1 antibody

Primary Antibodies
Applications Tested/Suitable for anti-HEXIM1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-HEXIM1 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:200-1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-HEXIM1 antibody

Detection of human HEXIM1 by immunohistochemistry. Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti-HEXIM1 (Cat. No. MBS3903407 Lot1, left image, MBS3903408 Lot1, middle image and Cat. No. MBS3903409 Lot1, right image) used at a dilution of 1:200 (1ug/ml) (MBS3903407-1) or 1:1,000 (1ug/ml) (MBS3903408-1 and MBS3903409-1). Detection: DAB. Counterstain: hematoxylin (blue).
Rabbit Anti-HEXIM1 Polyclonal Antibody – Cat# MBS3903408

Immunohistochemistry (IHC) of anti-HEXIM1 antibody

Detection of mouse HEXIM1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-HEXIM1 (Cat. No. MBS3903408 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB
Rabbit Anti-HEXIM1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3903408

Western Blot (WB) of anti-HEXIM1 antibody

Detection of human HEXIM1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HEXIM1 antibody MBS3903408 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies MBS3903407 and MBS3903409, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).
Rabbit Anti-HEXIM1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3903408

Other Notes

Small volumes of anti-HEXIM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HEXIM1 purchase

MBS3903408 is an antibody targeting HEXIM1. Also known as Rabbit anti-HEXIM1 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HEXIM1. It may not necessarily be applicable to this product.

NCBI GI #
5453682
NCBI GeneID
10614
NCBI Accession #
NP_006451.1
UniProt Primary Accession #
O94992
UniProt Secondary Accession #
B2R8Y5
UniProt Related Accession #
O94992
GenBank Nucleotide #
NM_006460.2
NCBI Official Full Name
protein HEXIM1
NCBI Official Synonym Full Names
hexamethylene bis-acetamide inducible 1
NCBI Official Symbol
HEXIM1
NCBI Official Synonym Symbols
CLP1; EDG1; HIS1; MAQ1
UniProt Protein Name
Protein HEXIM1
UniProt Synonym Protein Names
Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Hexamethylene bis-acetamide-inducible protein 1; Menage a quatre protein 1
Protein Family
Protein
UniProt Gene Name
HEXIM1
UniProt Synonym Gene Names
CLP1; EDG1; HIS1; MAQ1
UniProt Entry Name
HEXI1_HUMAN
NCBI Protein Information
protein HEXIM1; HMBA-inducible; menage a quatre 1; cardiac lineage protein 1; menage a quatre protein 1; estrogen down-regulated gene 1 protein; hexamthylene bis-acetamide inducible 1; hexamethylene bisacetamide-inducible protein; hexamethylene bis-acetam

NCBI Summary for HEXIM1

Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008]

UniProt Comments for HEXIM1

HEXIM1: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA. Up-regulated by HMBA (hexamethylene bisacetamide). Down-regulated by estrogen. Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues. Belongs to the HEXIM family.

Protein type: Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; snRNA binding

Biological Process: transcription, DNA-dependent; heart development; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
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Research Articles on HEXIM1

1. results not only identify HEXIM1 as a positive regulator of p53, but also propose a novel molecular mechanism of p53 activation caused by the anti-cancer drugs and compounds.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HEXIM1 antibody

 Reference Product  PubMed Publications
 CDK9 antibody  >41 publications with HEXIM1 and CDK9
 CCNT1 antibody  >20 publications with HEXIM1 and CCNT1
 CCNT2 antibody  >3 publications with HEXIM1 and CCNT2

Diseases associated with anti-HEXIM1 antibody

 Disease Name  Pubmed Publications
 Hypertrophy Antibodies  >15 publications with HEXIM1 and Hypertrophy
 Neoplasms Antibodies  >13 publications with HEXIM1 and Neoplasms
 Heart Diseases Antibodies  >9 publications with HEXIM1 and Heart Diseases
 Disease Models, Animal Antibodies  >4 publications with HEXIM1 and Disease Models, Animal
 Cardiomyopathies Antibodies  >3 publications with HEXIM1 and Cardiomyopathies
 Muscular Diseases Antibodies  >2 publications with HEXIM1 and Muscular Diseases
 Hyperplasia Antibodies  >1 publications with HEXIM1 and Hyperplasia
 Drug Toxicity Antibodies  >1 publications with HEXIM1 and Drug Toxicity
 Abnormalities, Drug-Induced Antibodies  >1 publications with HEXIM1 and Abnormalities, Drug-Induced
 Necrosis Antibodies  >1 publications with HEXIM1 and Necrosis

Organs/Tissues associated with anti-HEXIM1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >15 publications with HEXIM1 and Muscle
 Heart Antibodies  >11 publications with HEXIM1 and Heart
 Vascular Antibodies  >7 publications with HEXIM1 and Vascular
 Embryonic Tissue Antibodies  >4 publications with HEXIM1 and Embryonic Tissue
 Mammary Gland Antibodies  >3 publications with HEXIM1 and Mammary Gland
 Bone Antibodies  >1 publications with HEXIM1 and Bone
 Liver Antibodies  >1 publications with HEXIM1 and Liver
 Prostate Antibodies  >1 publications with HEXIM1 and Prostate
 Lung Antibodies  >1 publications with HEXIM1 and Lung
 Kidney Antibodies  >1 publications with HEXIM1 and Kidney
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