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Histone H1.3 (H1.3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9629023

MBS9629023 is an antibody targeting H1.3. Also known as Histone H1.3 Antibody. Reactive in Human, Mouse. Host: Rabbit. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histone H1.3, Polyclonal Antibody
Full Product Name
Histone H1.3 Antibody
Product Gene Name
Product Synonym Names
H1 histone family member 4; H1.4; H14_HUMAN; H1E; H1F4; Hist1h1e; Histone 1 H1e; Histone cluster 1 H1e; Histone H1; Histone H1.4; Histone H1B; MGC116819; H1 histone family member 3; H1.3; H13_HUMAN; H1F3; HIST1 H1D; HIST1H1D; Histone 1 H1d; Histone cluster 1 H1d; Histone H1.3; Histone H1c; MGC138176
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
affinity chromatography
Concentration
1mg/ml (lot specific)
Specificity
Histone H1.3 Antibody detects endogenous levels of total Histone H1.3.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
H1.3 peptide (MBS9631421) is used for blocking the activity of H1.3 antibody (MBS9629023)
Molecular Weight
21,865 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (26156559..26157343). Location: 6p21.3
OMIM
142220
3D Structure
ModBase 3D Structure for P10412
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human Histone H1.3.
Conjugation
Unconjugated
Similarity
The C-terminal domain is required for high-affinity binding to chromatin. Belongs to the histone H1/H5 family.
Post Translational Modifications
H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter.Citrullination at Arg-55 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.
Function
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Note
According to PubMed:15911621 more commonly found in euchromatin. According to PubMed:10997781 is associated with inactive chromatin.

Product Categories/Family for anti-H1.3 antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-H1.3 antibody
Western Blot (WB), ELISA (EIA)(peptide)
Application Notes for anti-H1.3 antibody
WB: 1:500-1:2000
ELISA(peptide): 1:20000-1:40000

Western Blot (WB) of anti-H1.3 antibody

Western blot analysis of extracts from various samples, using Histone H1.3 Antibody.
Lane 1: Serum starvation treated P19 cells, blocked with antigen-specific peptides,
Lane 2: Serum starvation treated P19 cells,
Lane 3: Heat-shock treated 293 cells.
Rabbit Anti-H1.3 Polyclonal Antibody – Cat# MBS9629023

Other Notes

Small volumes of anti-H1.3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for H1.3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone H1.4
NCBI Official Synonym Full Names
histone cluster 1, H1e
NCBI Official Symbol
HIST1H1E
NCBI Official Synonym Symbols
H1E; H1.4; H1F4; H1s-4; dJ221C16.5
UniProt Protein Name
Histone H1.4
UniProt Synonym Protein Names
Histone H1b
Protein Family
UniProt Gene Name
HIST1H1E
UniProt Synonym Gene Names
H1F4
UniProt Entry Name
H14_HUMAN
NCBI Protein Information
histone H1.4; histone H1b; histone 1, H1e; H1 histone family, member 4

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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