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IGF2BP2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9232158

Description: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)Background: This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions… More details

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Testing Data View PDF
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Product Overview

Product Name
IGF2BP2, Polyclonal Antibody
Full Product Name
IGF2BP2 Antibody (C-term)
Product Gene Name
Product Synonym Names
Insulin-like growth factor 2 mRNA-binding protein 2; IGF2 mRNA-binding protein 2; IMP-2; Hepatocellular carcinoma autoantigen p62; IGF-II mRNA-binding protein 2; VICKZ family member 2; IGF2BP2; IMP2; VICKZ2
Clonality
Polyclonal
Isotype
Ig
Host
Rabbit
Species Reactivity
Human
Predicted: Mouse
Specificity
This IGF2BP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 530-556 amino acids from the C-terminal region of human IGF2BP2.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
66121
OMIM
608289
3D Structure
ModBase 3D Structure for Q9Y6M1
Antigen Source
Human
Antigen Region
530-556
Antigen Type
Synthesized Peptide
Applications Tested/Suitable for anti-IGF2BP2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-IGF2BP2 antibody
WB: 1:2000
IHC-P: 1:50-100
FC/FACS: 1:10-50

Western Blot (WB) of anti-IGF2BP2 antibody

All lanes : Anti-IGF2BP2 Antibody (C-term) at 1:2000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: A549 whole cell lysate
Lane 4: Raji whole cell lysate
Lane 5: SH-SY5Y whole cell lysate

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 66 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-IGF2BP2 Polyclonal Antibody – Cat# MBS9232158

Immunohistochemistry (IHC)-Paraffin of anti-IGF2BP2 antibody

IGF2BP2 antibody(C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IGF2BP2 antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-IGF2BP2 Polyclonal Antibody, Immunohistochemistry (IHC)-Paraffin – Cat# MBS9232158

Flow Cytometry (FC/FACS) of anti-IGF2BP2 antibody

IGF2BP2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-IGF2BP2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS9232158

Product References and Citations for anti-IGF2BP2 antibody

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Pechlivanis, S., et al. Arterioscler. Thromb. Vasc. Biol. 30(9):1867-1872(2010). Heni, M., et al. Diabetes (2010). Rodriguez, S., et al. Growth Horm. IGF Res. 20(4):310-318(2010). Voight, B.F., et al. Nat. Genet. 42(7):579-589(2010).

Other Notes

Small volumes of anti-IGF2BP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IGF2BP2 purchase

MBS9232158 is an antibody targeting IGF2BP2. Also known as IGF2BP2 Antibody (C-term). Reactive in Human
Predicted: Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IGF2BP2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
insulin-like growth factor 2 mRNA-binding protein 2 isoform b
NCBI Official Synonym Full Names
insulin like growth factor 2 mRNA binding protein 2
NCBI Official Symbol
IGF2BP2
NCBI Official Synonym Symbols
IMP2; IMP-2; VICKZ2
UniProt Protein Name
Insulin-like growth factor 2 mRNA-binding protein 2
UniProt Synonym Protein Names
Hepatocellular carcinoma autoantigen p62; IGF-II mRNA-binding protein 2; VICKZ family member 2
UniProt Gene Name
IGF2BP2
UniProt Synonym Gene Names
IMP2; VICKZ2; IGF2 mRNA-binding protein 2; IMP-2
UniProt Entry Name
IF2B2_HUMAN
NCBI Protein Information
insulin-like growth factor 2 mRNA-binding protein 2

NCBI Summary for IGF2BP2

This gene encodes a protein that binds the 5' UTR of insulin-like growth factor 2 (IGF2) mRNA and regulates its translation. It plays an important role in metabolism and variation in this gene is associated with susceptibility to diabetes. Alternative splicing and promoter usage results in multiple transcript variants. Related pseudogenes are found on several chromosomes. [provided by RefSeq, Sep 2016]
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UniProt Comments for IGF2BP2

IGF2BP2: Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. Belongs to the RRM IMP/VICKZ family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 3q27.2

Cellular Component: cytoskeleton; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; mRNA 3'-UTR binding; nucleotide binding; translation regulator activity; mRNA 5'-UTR binding

Biological Process: anatomical structure morphogenesis; mRNA transport; negative regulation of translation; gene expression; regulation of cytokine biosynthetic process

Disease: Diabetes Mellitus, Noninsulin-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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