• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

IL5RA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6013593

IL5RA is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IL5RA, Polyclonal Antibody
Full Product Name
IL5RA, NT (IL5RA, IL5R, Interleukin-5 receptor subunit alpha, CDw125, CD125)
Product Gene Name
Product Synonym Names
Anti -IL5RA, NT (IL5RA, IL5R, Interleukin-5 receptor subunit alpha, CDw125, CD125)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
47,685 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (3108008..3152058, complement). Location: 3p26-p24
OMIM
147851
3D Structure
ModBase 3D Structure for Q01344
Immunogen
IL5RA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-87 amino acids from the N-terminal region of human IL5RA.

Product Categories/Family for anti-IL5RA antibody

Applications Tested/Suitable for anti-IL5RA antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-IL5RA antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-IL5RA antibody

Western Blot analysis of MDA-MB-453 cell line,lysate (35ug/ml) using MBS6013593 at 1:1000.
Rabbit Anti-IL5RA Polyclonal Antibody – Cat# MBS6013593

Immunohistochemistry (IHC) of anti-IL5RA antibody

Immunohistochemistry analysis of formalin fixed and paraffin embedded human lung carcinoma using MBS6013593 followed by peroxidase conjugation of the secondary antibody and DAB staining.
Rabbit Anti-IL5RA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6013593

Flow Cytometry (FC/FACS) of anti-IL5RA antibody

Flow cytometric analysis of U251 cells using MBS6013593 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-IL5RA Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6013593

Other Notes

Small volumes of anti-IL5RA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL5RA purchase

MBS6013593 is an antibody targeting IL5RA. Also known as IL5RA, NT (IL5RA, IL5R, Interleukin-5 receptor subunit alpha, CDw125, CD125). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IL5RA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin-5 receptor subunit alpha isoform 4
NCBI Official Synonym Full Names
interleukin 5 receptor, alpha
NCBI Official Symbol
IL5RA
NCBI Official Synonym Symbols
IL5R; CD125; CDw125; HSIL5R3
UniProt Protein Name
Interleukin-5 receptor subunit alpha
UniProt Synonym Protein Names
CDw125
UniProt Gene Name
IL5RA
UniProt Synonym Gene Names
IL5R; IL-5 receptor subunit alpha; IL-5R subunit alpha; IL-5R-alpha; IL-5RA
UniProt Entry Name
IL5RA_HUMAN
NCBI Protein Information
interleukin-5 receptor subunit alpha; CD125 antigen; IL-5R subunit alpha; IL-5 receptor subunit alpha; interleukin 5 receptor type 3; interleukin-5 receptor alpha chain

NCBI Summary for IL5RA

The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported. [provided by RefSeq, Jul 2011]
Show more

UniProt Comments for IL5RA

IL5RA: This is the receptor for interleukin-5. The alpha chain binds to IL5. Belongs to the type I cytokine receptor family. Type 5 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p26-p24

Cellular Component: extracellular space; integral to membrane; plasma membrane

Molecular Function: protein binding; interleukin-5 receptor activity

Biological Process: cell proliferation; regulation of interleukin-5 production; inflammatory response to antigenic stimulus; signal transduction
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000