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KLF5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS629719

KLF5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, the protein is thought to be a transcription factor. More details

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Product Overview

Online Datasheet in PDF
Product Name
KLF5, Polyclonal Antibody
Full Product Name
KLF5, CT (Kruppel-like Factor 5, Basic Transcription Element Binding Protein 2, BTE-binding Protein 2, BTEB2, Colon Krueppel-like Factor, CKLF, GC Box Binding Protein 2, Intestinal-enriched Krueppel-like Factor, IKLF, Transcription Factor BTEB2)
Product Gene Name
anti-KLF5 antibody
Product Synonym Names
Anti -KLF5, CT (Kruppel-like Factor 5, Basic Transcription Element Binding Protein 2, BTE-binding Protein 2, BTEB2, Colon Krueppel-like Factor, CKLF, GC Box Binding Protein 2, Intestinal-enriched Krueppel-like Factor, IKLF, Transcription Factor BTEB2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human KLF5. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
50,792 Da
Chromosome Location
Chromosome: 13; NC_000013.10 (73633142..73651676). Location: 13q22.1
OMIM
602903
3D Structure
ModBase 3D Structure for Q13887
Immunogen
Synthetic peptide selected from the C-terminal region of human KLF5 (KLH).

Product Categories/Family for anti-KLF5 antibody

Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-KLF5 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-KLF5 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-KLF5 antibody

KLF5 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the KLF5 antibody detected the KLF5 protein (arrow).
Rabbit Anti-KLF5 Polyclonal Antibody – Cat# MBS629719

Immunofluorescence (IF) of anti-KLF5 antibody

Fluorescent confocal image of U251 cell stained with KLF5 Antibody (C-term) .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF5 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). KLF5 immunoreactivity is localized to vesicles and Nucleus significantly.
Rabbit Anti-KLF5 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS629719

Immunohistochemistry (IHC) of anti-KLF5 antibody

KLF5 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KLF5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-KLF5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS629719

Flow Cytometry (FC/FACS) of anti-KLF5 antibody

KLF5 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-KLF5 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS629719

Other Notes

Small volumes of anti-KLF5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLF5 purchase

MBS629719 is an antibody targeting KLF5. Also known as KLF5, CT (Kruppel-like Factor 5, Basic Transcription Element Binding Protein 2, BTE-binding Protein 2, BTEB2, Colon Krueppel-like Factor, CKLF, GC Box Binding Protein 2, Intestinal-enriched Krueppel-like Factor, IKLF, Transcription Factor BTEB2). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KLF5. It may not necessarily be applicable to this product.

NCBI GI #
14251215
NCBI GeneID
688
NCBI Accession #
NP_001721.2
UniProt Primary Accession #
Q13887
UniProt Secondary Accession #
Q9UHP8
UniProt Related Accession #
Q13887; Q5T6X2; Q9HBZ3
GenBank Nucleotide #
NM_001730.3
NCBI Official Full Name
Krueppel-like factor 5
NCBI Official Synonym Full Names
Kruppel-like factor 5 (intestinal)
NCBI Official Symbol
KLF5
NCBI Official Synonym Symbols
CKLF; IKLF; BTEB2
UniProt Protein Name
Krueppel-like factor 5
UniProt Synonym Protein Names
Basic transcription element-binding protein 2; BTE-binding protein 2; Colon krueppel-like factor; GC-box-binding protein 2; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2
Protein Family
Krueppel-like factor
UniProt Gene Name
KLF5
UniProt Synonym Gene Names
BTEB2; CKLF; IKLF
UniProt Entry Name
KLF5_HUMAN
NCBI Protein Information
Krueppel-like factor 5; OTTHUMP00000018502; BTE-binding protein 2; GC box binding protein 2; GC-box-binding protein 2; colon kruppel-like factor; colon krueppel-like factor; transcription factor BTEB2; intestinal-enriched kruppel-like factor; intestinal-enriched krueppel-like factor; basic transcription element binding protein 2; basic transcription element-binding protein 2

NCBI Summary for KLF5

This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor. [provided by RefSeq]

UniProt Comments for KLF5

KLF5: Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 13q22.1

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; cytoplasm; plasma membrane

Molecular Function: protein binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; microvillus biogenesis; positive regulation of cell proliferation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; angiogenesis; regulation of microvillus biogenesis
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Research Articles on KLF5

1. while KLF5 is not required for oncogenic mutant K-Ras-induced lung tumorigenesis, KLF5 regulation of ABCG2 expression may be important for chemotherapeutic resistance and patient survival.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KLF5 antibody

 Reference Product  PubMed Publications
 SET antibody  >9 publications with KLF5 and SET
 RARA antibody  >1 publications with KLF5 and RARA
 EP300 antibody  >1 publications with KLF5 and EP300
 CREBBP antibody  >1 publications with KLF5 and CREBBP

Pathways associated with anti-KLF5 antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Diabetes Pathways antibodies  Diabetes Pathways Diagram
 Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies  Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram

Diseases associated with anti-KLF5 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >58 publications with KLF5 and Neoplasms
 Intestinal Neoplasms Antibodies  >14 publications with KLF5 and Intestinal Neoplasms
 Vascular Diseases Antibodies  >12 publications with KLF5 and Vascular Diseases
 Colorectal Neoplasms Antibodies  >12 publications with KLF5 and Colorectal Neoplasms
 Carcinoma Antibodies  >11 publications with KLF5 and Carcinoma
 Breast Neoplasms Antibodies  >11 publications with KLF5 and Breast Neoplasms
 Prostatic Neoplasms Antibodies  >10 publications with KLF5 and Prostatic Neoplasms
 Prostatic Diseases Antibodies  >10 publications with KLF5 and Prostatic Diseases
 Genital Diseases, Male Antibodies  >10 publications with KLF5 and Genital Diseases, Male
 Leukemia Antibodies  >8 publications with KLF5 and Leukemia

Organs/Tissues associated with anti-KLF5 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >42 publications with KLF5 and Muscle
 Intestine Antibodies  >22 publications with KLF5 and Intestine
 Blood Antibodies  >12 publications with KLF5 and Blood
 Embryonic Tissue Antibodies  >11 publications with KLF5 and Embryonic Tissue
 Prostate Antibodies  >11 publications with KLF5 and Prostate
 Skin Antibodies  >8 publications with KLF5 and Skin
 Lung Antibodies  >6 publications with KLF5 and Lung
 Bladder Antibodies  >5 publications with KLF5 and Bladder
 Bone Antibodies  >5 publications with KLF5 and Bone
 Kidney Antibodies  >4 publications with KLF5 and Kidney
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