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KLHL3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9608989

Function: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron (PubMed:14528312, PubMed:22406640, PubMed:23387299, PubMed:23453970, PubMed:23576762, PubMed:23665031).… More details

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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
KLHL3, Polyclonal Antibody
Full Product Name
KLHL3 Antibody
Product Gene Name
anti-KLHL3 antibody
Product Synonym Names
FLJ40871; kelch (Drosophila) like 3; kelch like 3 (Drosophila); kelch like 3; Kelch like protein 3; KIAA1129; KLHL 3; KLHL3; MGC44594
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
KLHL3 antibody detects endogenous levels of total KLHL3
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
KLHL3 peptide (MBS9627701) is used for blocking the activity of KLHL3 antibody (MBS9608989)
Molecular Weight
Observed: 64 kDa
Predicted: 65 kDa
3D Structure
ModBase 3D Structure for Q9UH77
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human KLHL3
Subcellular Location
Cytoplasmic and Cytoskeleton.
Tissue Specificity
Widely expressed.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Rabbit
Similarity
Bovine (91%), Horse (80%), Sheep (91%), Rabbit (100%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-KLHL3 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-KLHL3 antibody
WB: 1:1000-3000
IHC: 1:200
IF/ICC: 1:100-1:500

Immunohistochemistry (IHC) of anti-KLHL3 antibody

MBS9608989 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-KLHL3 Polyclonal Antibody – Cat# MBS9608989

Western Blot (WB) of anti-KLHL3 antibody

Western blot analysis of KLHL3 using Jurkat whole cell lysates
Rabbit Anti-KLHL3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9608989

Immunofluorescence (IF) of anti-KLHL3 antibody

MBS9608989 staining RAW264.7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-KLHL3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9608989

Other Notes

Small volumes of anti-KLHL3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLHL3 purchase

MBS9608989 is an antibody targeting KLHL3. Also known as KLHL3 Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KLHL3. It may not necessarily be applicable to this product.

NCBI GI #
380692310
NCBI GeneID
26249
NCBI Accession #
NP_001244123.1
UniProt Primary Accession #
Q9UH77
UniProt Secondary Accession #
Q9UH75; Q9UH76; Q9ULU0; Q9Y6V6; B2RBK7
UniProt Related Accession #
Q9UH77
GenBank Nucleotide #
NM_001257194.1
NCBI Official Full Name
kelch-like protein 3 isoform 2
NCBI Official Synonym Full Names
kelch like family member 3
NCBI Official Symbol
KLHL3
NCBI Official Synonym Symbols
PHA2D
UniProt Protein Name
Kelch-like protein 3
Protein Family
Kelch-like protein
UniProt Gene Name
KLHL3
NCBI Protein Information
kelch-like protein 3

NCBI Summary for KLHL3

This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
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UniProt Comments for KLHL3

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron (PubMed:14528312, PubMed:22406640, PubMed:23387299, PubMed:23453970, PubMed:23576762, PubMed:23665031). The BCR(KLHL3) complex acts by mediating ubiquitination of WNK4, an inhibitor of potassium channel KCNJ1, leading to WNK4 degradation (PubMed:23387299, PubMed:23453970, PubMed:23576762, PubMed:23665031). The BCR(KLHL3) complex also mediates ubiquitination and degradation of CLDN8, a tight-junction protein required for paracellular chloride transport in the kidney ().
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Research Articles on KLHL3

1. A new recessive mutation in KLHL3 (S553L) was identified in familial hyperkalemia and hypertension. Increased urinary NCC was found in affected members (heterozygous) with dominant KLHL3 Q309R, and in affected members (homozygous) of the recessive form.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KLHL3 antibody

 Reference Product  PubMed Publications
 CUL3 antibody  >18 publications with KLHL3 and CUL3

Diseases associated with anti-KLHL3 antibody

 Disease Name  Pubmed Publications
 Pseudohypoaldosteronism Antibodies  >20 publications with KLHL3 and Pseudohypoaldosteronism
 Cardiovascular Abnormalities Antibodies  >2 publications with KLHL3 and Cardiovascular Abnormalities
 Inflammation Antibodies  >1 publications with KLHL3 and Inflammation
 Disorders of Sex Development Antibodies  >1 publications with KLHL3 and Disorders of Sex Development
 Fibrosis Antibodies  >1 publications with KLHL3 and Fibrosis
 Congenital Abnormalities Antibodies  >1 publications with KLHL3 and Congenital Abnormalities

Organs/Tissues associated with anti-KLHL3 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >13 publications with KLHL3 and Kidney
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