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KRT18 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6484241

MBS6484241 is an antibody targeting KRT18. Also known as KRT18, CT (Keratin, Type I Cytoskeletal 18, Cytokeratin-18, CK-18, Keratin-18, K18, Cell Proliferation-inducing Gene 46 Protein, PIG46, CYK18) (Biotin). Reactive in Human, Mouse. Host: Rabbit. More details

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Product Overview

Online Datasheet in PDF
Product Name
KRT18, Polyclonal Antibody
Full Product Name
KRT18, CT (Keratin, Type I Cytoskeletal 18, Cytokeratin-18, CK-18, Keratin-18, K18, Cell Proliferation-inducing Gene 46 Protein, PIG46, CYK18) (Biotin)
Product Gene Name
anti-KRT18 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human CYK18. Species Crossreactivity: mouse.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
48,058 Da
OMIM
148070
3D Structure
ModBase 3D Structure for P05783
Immunogen
Synthetic peptide selected from the C-terminal region of human CYK18 (KLH).
Conjugate
Biotin
Note
Preservative Free

Product Categories/Family for anti-KRT18 antibody

Antibodies; Keratin; Cytokeratin
Applications Tested/Suitable for anti-KRT18 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes for anti-KRT18 antibody
ELISA: 1:1,000
WB: 1:50-1:100
FC/FACS: 1:10-1:50
IHC: 1:50-1:100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-KRT18 antibody

Western blot analysis of CYK18 Antibody (C-term) in K562,NCI-H460 cell line lysates and mouse stomach tissues lysates(35ug/lane). CYK18(arrow) was detected using the purified Pab.
Rabbit Anti-KRT18 Polyclonal Antibody – Cat# MBS6484241

Immunohistochemistry (IHC) of anti-KRT18 antibody

Formalin-fixed and paraffin-embedded human colon carcinoma with CYK18 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-KRT18 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6484241

Flow Cytometry (FC/FACS) of anti-KRT18 antibody

Flow cytometric analysis of WiDr cells using CYK18 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-KRT18 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6484241

Immunofluorescence (IF) of anti-KRT18 antibody

Confocal immunofluorescent analysis of CYK18 Antibody (C-term) with prostate carcinoma followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-KRT18 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6484241

Immunohistochemistry (IHC) of anti-KRT18 antibody

CYK18 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYK18 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-KRT18 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6484241

Product References and Citations for anti-KRT18 antibody

1. Zhang, Q., Clin. Cancer Res. 15(10), 3557-3567 (2009). 2. Kruse, R., Folia Histochem. Cytobiol. 47(1), 127-130 (2009). 3. Toivola, D.M., Hepatology 40(2), 459-466 (2004).

Other Notes

Small volumes of anti-KRT18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT18. It may not necessarily be applicable to this product.

NCBI GI #
4557888
NCBI GeneID
3875
NCBI Accession #
NP_000215
UniProt Primary Accession #
P05783
UniProt Secondary Accession #
Q53G38; Q5U0N8; Q9BW26
UniProt Related Accession #
P05783
GenBank Nucleotide #
NM_000224.2
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18
NCBI Official Symbol
KRT18
NCBI Official Synonym Symbols
K18; CK-18; CYK18
UniProt Protein Name
Keratin, type I cytoskeletal 18
UniProt Synonym Protein Names
Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18
Protein Family
Keratin
UniProt Gene Name
KRT18
UniProt Synonym Gene Names
CYK18; CK-18; K18
UniProt Entry Name
K1C18_HUMAN
NCBI Protein Information
keratin, type I cytoskeletal 18

NCBI Summary for KRT18

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT18

K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis.

Protein type: Cytoskeletal; Nucleolus

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: perinuclear region of cytoplasm; keratin filament; cytoplasm; nucleolus; microtubule organizing center; intermediate filament

Molecular Function: protein binding; structural molecule activity

Biological Process: anatomical structure morphogenesis; tumor necrosis factor-mediated signaling pathway; viral reproduction; intermediate filament cytoskeleton organization and biogenesis; cell cycle; Golgi to plasma membrane CFTR protein transport; negative regulation of apoptosis

Disease: Cirrhosis, Familial
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Research Articles on KRT18

1. FIB-4 index and CK-18F have good diagnostic abilities not only for nonalcoholic steatohepatitis (NASH) overall, but also for NASH with mild fibrosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT18 antibody

 Reference Product  PubMed Publications
 KRT8 antibody  >39 publications with KRT18 and KRT8
 CASP3 antibody  >5 publications with KRT18 and CASP3
 KRT5 antibody  >3 publications with KRT18 and KRT5
 EGFR antibody  >3 publications with KRT18 and EGFR
 DNAJB6 antibody  >1 publications with KRT18 and DNAJB6
 CASP7 antibody  >1 publications with KRT18 and CASP7
 CASP6 antibody  >1 publications with KRT18 and CASP6

Pathways associated with anti-KRT18 antibody

 Products by Pathway  Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Keratinization Pathway antibodies  Keratinization Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram

Diseases associated with anti-KRT18 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >41 publications with KRT18 and Neoplasms
 Liver Diseases Antibodies  >28 publications with KRT18 and Liver Diseases
 Carcinoma Antibodies  >23 publications with KRT18 and Carcinoma
 Adenocarcinoma Antibodies  >13 publications with KRT18 and Adenocarcinoma
 Breast Neoplasms Antibodies  >12 publications with KRT18 and Breast Neoplasms
 Fibrosis Antibodies  >12 publications with KRT18 and Fibrosis
 Fatty Liver Antibodies  >9 publications with KRT18 and Fatty Liver
 Liver Cirrhosis Antibodies  >8 publications with KRT18 and Liver Cirrhosis
 Neoplasm Metastasis Antibodies  >8 publications with KRT18 and Neoplasm Metastasis
 Liver Neoplasms Antibodies  >7 publications with KRT18 and Liver Neoplasms

Organs/Tissues associated with anti-KRT18 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >34 publications with KRT18 and Liver
 Blood Antibodies  >18 publications with KRT18 and Blood
 Eye Antibodies  >6 publications with KRT18 and Eye
 Muscle Antibodies  >6 publications with KRT18 and Muscle
 Kidney Antibodies  >6 publications with KRT18 and Kidney
 Prostate Antibodies  >5 publications with KRT18 and Prostate
 Vascular Antibodies  >4 publications with KRT18 and Vascular
 Lymph Antibodies  >4 publications with KRT18 and Lymph
 Lymph Node Antibodies  >4 publications with KRT18 and Lymph Node
 Bladder Antibodies  >4 publications with KRT18 and Bladder
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