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MAPG8a/b Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6318747

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MAPG8a/b, Polyclonal Antibody
Full Product Name
MAPG8a/b (MAP1LC3A/B, MAP1LC3B, MAP1ALC3, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-a
Product Gene Name
anti-MAPG8a/b antibody
Product Synonym Gene Name
MAP1LC3B
Product Synonym Names
MAP1LC3A/B; MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 light chain 3 beta
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
125
Chromosome Location
Chromosome: 16; NC_000016.9 (87423157..87438380). Location: 16q24.2
OMIM
609604
3D Structure
ModBase 3D Structure for Q9GZQ8
Immunogen
Cleaved-APG8a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from human Cleaved-APG8a/b.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-MAPG8a/b antibody

Antibodies; Microtubule-associated Proteins
Applications Tested/Suitable for anti-MAPG8a/b antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-MAPG8a/b antibody
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Immunofluorescence (IF) of anti-MAPG8a/b antibody

Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP10648a APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Rabbit Anti-MAPG8a/b Polyclonal Antibody – Cat# MBS6318747

Western Blot (WB) of anti-MAPG8a/b antibody

APG8a/b (MAP1LC3A/B) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the MAP1LC3A antibody detected the MAP1LC3A protein (arrow).
Rabbit Anti-MAPG8a/b Polyclonal Antibody, Western Blot (WB) – Cat# MBS6318747

Immunohistochemistry (IHC) of anti-MAPG8a/b antibody

Cleaved-APG8a/b antibody (MAP1LC3A/B) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Cleaved-APG8a/b antibody (MAP1LC3A/B) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-MAPG8a/b Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6318747

Product References and Citations for anti-MAPG8a/b antibody

Rouschop, K.M., et al. J. Clin. Invest. 120(1):127-141(2010). Kirkin, V., et al. Mol. Cell 33(4):505-516(2009). Othman, E.Q., et al. J. Clin. Lab. Anal. 23(4):249-258(2009). Liu, Q., et al. Ai Zheng 27(1):25-29(2008). Komatsu, M., et al. Cell 131(6):1149-1163(2007).

Other Notes

Small volumes of anti-MAPG8a/b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAPG8a/b purchase

MBS6318747 is an antibody targeting MAPG8a/b. Also known as MAPG8a/b (MAP1LC3A/B, MAP1LC3B, MAP1ALC3, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-a. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MAPG8a/b. It may not necessarily be applicable to this product.

NCBI GI #
12383056
NCBI GeneID
81631
NCBI Accession #
NP_073729.1
UniProt Primary Accession #
Q9GZQ8
UniProt Secondary Accession #
Q6NW02
UniProt Related Accession #
Q9GZQ8
GenBank Nucleotide #
NM_022818.4
NCBI Official Full Name
microtubule-associated proteins 1A/1B light chain 3B
NCBI Official Synonym Full Names
microtubule-associated protein 1 light chain 3 beta
NCBI Official Symbol
MAP1LC3B
NCBI Official Synonym Symbols
LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
UniProt Protein Name
Microtubule-associated proteins 1A/1B light chain 3B
UniProt Synonym Protein Names
Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta
UniProt Gene Name
MAP1LC3B
UniProt Synonym Gene Names
MAP1ALC3; MAP1A/MAP1B LC3 B
UniProt Entry Name
MLP3B_HUMAN
NCBI Protein Information
microtubule-associated proteins 1A/1B light chain 3B; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1 light chain 3-like protein 2; autophagy-related ubiquitin-like modifier LC3 B

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAPG8a/b antibody

 Reference Product  PubMed Publications
 ATG5 antibody  >9 publications with MAPG8a/b and ATG5
 SQSTM1 antibody  >8 publications with MAPG8a/b and SQSTM1
 ATG7 antibody  >7 publications with MAPG8a/b and ATG7
 BECN1 antibody  >6 publications with MAPG8a/b and BECN1
 ATF4 antibody  >3 publications with MAPG8a/b and ATF4
 ATG3 antibody  >2 publications with MAPG8a/b and ATG3
 ATG4B antibody  >2 publications with MAPG8a/b and ATG4B
 BNIP3 antibody  >1 publications with MAPG8a/b and BNIP3

Pathways associated with anti-MAPG8a/b antibody

 Products by Pathway  Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram

Diseases associated with anti-MAPG8a/b antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >23 publications with MAPG8a/b and Neoplasms
 Breast Neoplasms Antibodies  >8 publications with MAPG8a/b and Breast Neoplasms
 Adenocarcinoma Antibodies  >6 publications with MAPG8a/b and Adenocarcinoma
 Nervous System Diseases Antibodies  >5 publications with MAPG8a/b and Nervous System Diseases
 Necrosis Antibodies  >4 publications with MAPG8a/b and Necrosis
 Inflammation Antibodies  >3 publications with MAPG8a/b and Inflammation
 Drug Toxicity Antibodies  >2 publications with MAPG8a/b and Drug Toxicity
 Kidney Diseases Antibodies  >2 publications with MAPG8a/b and Kidney Diseases
 Lung Neoplasms Antibodies  >2 publications with MAPG8a/b and Lung Neoplasms
 Neoplasm Metastasis Antibodies  >2 publications with MAPG8a/b and Neoplasm Metastasis

Organs/Tissues associated with anti-MAPG8a/b antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >5 publications with MAPG8a/b and Lung
 Blood Antibodies  >5 publications with MAPG8a/b and Blood
 Skin Antibodies  >4 publications with MAPG8a/b and Skin
 Lymph Node Antibodies  >4 publications with MAPG8a/b and Lymph Node
 Kidney Antibodies  >4 publications with MAPG8a/b and Kidney
 Lymph Antibodies  >4 publications with MAPG8a/b and Lymph
 Brain Antibodies  >4 publications with MAPG8a/b and Brain
 Embryonic Tissue Antibodies  >3 publications with MAPG8a/b and Embryonic Tissue
 Eye Antibodies  >3 publications with MAPG8a/b and Eye
 Muscle Antibodies  >3 publications with MAPG8a/b and Muscle
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