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MED17 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9604910

Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA… More details

$325 / 0.1 mL +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MED17, Polyclonal Antibody
Full Product Name
MED17 Antibody
Product Gene Name
anti-MED17 antibody
Product Synonym Names
77-KD subunit; Activator recruited cofactor 77 kDa component; Activator-recruited cofactor 77 kDa component; ARC77; Cofactor required for Sp1 transcriptional activation subunit 6; cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa; CRSP complex subunit 6; CRSP6; CRSP77; DRIP80; med17; MED17_HUMAN; Mediator complex subunit 17; Mediator of RNA polymerase II transcription subunit 17; Mediator of RNA polymerase II transcription subunit 17, S cerevisiae, homolog of; Thyroid hormone receptor-associated protein complex 80 kDa component; Thyroid hormone receptor-associated protein, 80-KD subunit; Transcriptional coactivator CRSP77; Trap80; VITAMIN D RECEPTOR-INTERACTING PROTEIN, 80-KD; Vitamin D3 receptor-interacting protein complex 80 kDa component
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
MED17 antibody detects endogenous levels of total MED17
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
MED17 peptide (MBS9623627) is used for blocking the activity of MED17 antibody (MBS9604910)
Molecular Weight
Observed: 73 kD
Predicted: 73 kDa
3D Structure
ModBase 3D Structure for Q9NVC6
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Tissue Specificity
Ubiquitous.
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MED17 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-MED17 antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-MED17 antibody

MBS9604910 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-MED17 Polyclonal Antibody – Cat# MBS9604910

Western Blot (WB) of anti-MED17 antibody

Western blot analysis of extracts from HT-29 cells, using MED17 antibody.The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-MED17 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9604910

Other Notes

Small volumes of anti-MED17 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MED17 purchase

MBS9604910 is an antibody targeting MED17. Also known as MED17 Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MED17. It may not necessarily be applicable to this product.

NCBI GI #
28558975
NCBI GeneID
9440
NCBI Accession #
NP_004259.3
UniProt Primary Accession #
Q9NVC6
UniProt Secondary Accession #
Q9HA81; Q9UNP7; Q9Y2W0; Q9Y660; B3KN07
UniProt Related Accession #
Q9NVC6
GenBank Nucleotide #
NM_004268.4
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 17
NCBI Official Synonym Full Names
mediator complex subunit 17
NCBI Official Symbol
MED17
NCBI Official Synonym Symbols
SRB4; CRSP6; CRSP77; DRIP80; TRAP80
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 17
UniProt Synonym Protein Names
Activator-recruited cofactor 77 kDa component; ARC77; Cofactor required for Sp1 transcriptional activation subunit 6; CRSP complex subunit 6; Mediator complex subunit 17; Thyroid hormone receptor-associated protein complex 80 kDa component; Trap80; Transcriptional coactivator CRSP77; Vitamin D3 receptor-interacting protein complex 80 kDa component; DRIP80
Protein Family
Mediator of RNA polymerase II transcription
UniProt Gene Name
MED17
UniProt Synonym Gene Names
ARC77; CRSP6; DRIP77; DRIP80; TRAP80; ARC77; CRSP complex subunit 6; Trap80; DRIP80
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 17

NCBI Summary for MED17

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq, Jul 2008]
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UniProt Comments for MED17

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
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Research Articles on MED17

1. These findings suggest that hMED17 may play essential roles in switching between transcription and nucleotide excision repair.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MED17 antibody

 Reference Product  PubMed Publications
 MED8 antibody  >4 publications with MED17 and MED8
 MED22 antibody  >2 publications with MED17 and MED22

Pathways associated with anti-MED17 antibody

 Products by Pathway  Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 PPARA Activates Gene Expression Pathway antibodies  PPARA Activates Gene Expression Pathway Diagram
 Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway antibodies  Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway Diagram
 Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies  Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram

Diseases associated with anti-MED17 antibody

 Disease Name  Pubmed Publications
 Prostatic Diseases Antibodies  >2 publications with MED17 and Prostatic Diseases
 Musculoskeletal Diseases Antibodies  >1 publications with MED17 and Musculoskeletal Diseases
 Craniofacial Abnormalities Antibodies  >1 publications with MED17 and Craniofacial Abnormalities
 Atrophy Antibodies  >1 publications with MED17 and Atrophy
 Nervous System Diseases Antibodies  >1 publications with MED17 and Nervous System Diseases
 Seizures Antibodies  >1 publications with MED17 and Seizures
 Necrosis Antibodies  >1 publications with MED17 and Necrosis
 Inflammation Antibodies  >1 publications with MED17 and Inflammation

Organs/Tissues associated with anti-MED17 antibody

 Organ/Tissue Name  Pubmed Publications
 Thyroid Antibodies  >4 publications with MED17 and Thyroid
 Prostate Antibodies  >2 publications with MED17 and Prostate
 Brain Antibodies  >2 publications with MED17 and Brain
 Ganglia Antibodies  >1 publications with MED17 and Ganglia
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