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MMP15 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6487119

MBS6487119 is an antibody targeting MMP15. Also known as MMP15, NT (Matrix Metalloproteinase-15, MMP-15, Membrane-type Matrix Metalloproteinase 2, MT-MMP 2, MTMMP2, Membrane-type-2 Matrix Metalloproteinase, MT2-MMP, MT2MMP, SMCP-2) (Biotin). Reactive in… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MMP15, Polyclonal Antibody
Full Product Name
MMP15, NT (Matrix Metalloproteinase-15, MMP-15, Membrane-type Matrix Metalloproteinase 2, MT-MMP 2, MTMMP2, Membrane-type-2 Matrix Metalloproteinase, MT2-MMP, MT2MMP, SMCP-2) (Biotin)
Product Gene Name
Product Synonym Gene Name
MM15
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Specificity
Recognizes human MMP15. Species Crossreactivity: mouse.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
75.807
Immunogen
Synthetic peptide selected from the N-terminal region of human MMP15 (KLH).
Conjugate
Biotin
Note
Preservative Free
Applications Tested/Suitable for anti-MMP15 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes for anti-MMP15 antibody
ELISA: 1:1,000
WB: 1:100-1:500
FC/FACS: 1:10-1:50
IHC: 1:50-1:100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MMP15 antibody

The anti-MMP15 N-term Antibody is used in Western blot to detect MMP15 in mouse brain tissue lysate (lane 1) and HL60 cell lysate (lane 2) lysate.
Rabbit Anti-MMP15 Polyclonal Antibody – Cat# MBS6487119

Immunohistochemistry (IHC) of anti-MMP15 antibody

Formalin-fixed and paraffin-embedded human colon carcinoma with MMP15 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-MMP15 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6487119

Flow Cytometry (FC/FACS) of anti-MMP15 antibody

Flow cytometric analysis of WiDr cells using MMP15 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-MMP15 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6487119

Product References and Citations for anti-MMP15 antibody

1. Jung, M., et al., Prostate 55(2):89-98 (2003). 2. Nagase, H., et al., J. Biol. Chem. 274(31):21491-21494 (1999). 3. d'Ortho, M.P., et al., Eur. J. Biochem. 250(3):751-757 (1997). 4. Sato, H., et al., Genomics 39(3):412-413 (1997). 5. Mattei, M.G., et al., Genomics 40(1):168-169 (1997).

Other Notes

Small volumes of anti-MMP15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MMP15. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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