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MPST Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6488569

MBS6488569 is an antibody targeting MPST. Also known as MPST, NT (3-Mercaptopyruvate Sulfurtransferase, Mercaptopyruvate Sulfurtransferase, MST, MGC24539, TST2) (FITC). Reactive in Human, Mouse. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MPST, Polyclonal Antibody
Full Product Name
MPST, NT (3-Mercaptopyruvate Sulfurtransferase, Mercaptopyruvate Sulfurtransferase, MST, MGC24539, TST2) (FITC)
Product Gene Name
Product Synonym Names
EC=2.8.1.2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human MPST. Species Crossreactivity: mouse.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Molecular Weight
33kDa
OMIM
249650
3D Structure
ModBase 3D Structure for P25325
Immunogen
Synthetic peptide selected from the N-terminal region of human MPST (KLH).
Conjugate
FITC
Note
Preservative Free

Product Categories/Family for anti-MPST antibody

Applications Tested/Suitable for anti-MPST antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-MPST antibody
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MPST antibody

Western blot analysis of MPST antibody (N-term) in Hela cell line lysates (35ug/lane). MPST (arrow) was detected using the purified Pab.
Rabbit Anti-MPST Polyclonal Antibody – Cat# MBS6488569

Western Blot (WB) of anti-MPST antibody

Western blot analysis of MPST antibody (N-term) in mouse kidney tissue lysates (35ug/lane). MPST (arrow) was detected using the purified Pab.
Rabbit Anti-MPST Polyclonal Antibody, Western Blot (WB) – Cat# MBS6488569

Flow Cytometry (FC/FACS) of anti-MPST antibody

MPST Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-MPST Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6488569

Product References and Citations for anti-MPST antibody

1. Billaut-Laden, I., Toxicol. Lett. 165(2), 101-111 (2006).

Other Notes

Small volumes of anti-MPST antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MPST. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
3-mercaptopyruvate sulfurtransferase isoform 1
NCBI Official Synonym Full Names
mercaptopyruvate sulfurtransferase
NCBI Official Symbol
MPST
NCBI Official Synonym Symbols
MST; TST2; TUM1
UniProt Protein Name
3-mercaptopyruvate sulfurtransferase
UniProt Gene Name
MPST
UniProt Synonym Gene Names
TST2; MST
UniProt Entry Name
THTM_HUMAN
NCBI Protein Information
3-mercaptopyruvate sulfurtransferase

NCBI Summary for MPST

This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for MPST

MPST: Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. May have a role in cyanide degradation or in thiosulfate biosynthesis.

Protein type: Transferase; Amino Acid Metabolism - cysteine and methionine; EC 2.8.1.2; Mitochondrial

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: neuron projection; mitochondrial inner membrane; synapse; cell junction

Molecular Function: 3-mercaptopyruvate sulfurtransferase activity; thiosulfate sulfurtransferase activity

Biological Process: cyanate catabolic process; response to toxin
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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