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MRCKG Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9603927

Function: May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation (By similarity).Subunit Structure:… More details

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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MRCKG, Polyclonal Antibody
Full Product Name
MRCKG Antibody
Product Gene Name
Product Synonym Gene Name
CDC42BPG
Product Synonym Names
CDC42 binding protein kinase gamma; DMPK like gamma; DMPK2; MRCK gamma; Myotonic dystrophy kinase related CDC42 binding kinase gamma; Myotonic dystrophy protein kinase like alpha; Serine/threonine protein kinase MRCK gamma
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
MRCKG antibody detects endogenous levels of total MRCKG
Antibody/Peptide Pairs
MRCKG peptide (MBS9622644) is used for blocking the activity of MRCKG antibody (MBS9603927)
Molecular Weight
Predicted: 173 kDa
3D Structure
ModBase 3D Structure for Q6DT37
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm. Note: concentrates at the leading edge of cells.
Tissue Specificity
Expressed in heart and skeletal muscle.
Predicted Cross Reactivity
Sheep
Similarity
Sheep (82%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MRCKG antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-MRCKG antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Western Blot (WB) of anti-MRCKG antibody

Western blot analysis of MRCKG expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-MRCKG Polyclonal Antibody – Cat# MBS9603927

Immunohistochemistry (IHC) of anti-MRCKG antibody

MBS9603927 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-MRCKG Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9603927

Other Notes

Small volumes of anti-MRCKG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MRCKG purchase

MBS9603927 is an antibody targeting MRCKG. Also known as MRCKG Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MRCKG. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein kinase MRCK gamma
NCBI Official Synonym Full Names
CDC42 binding protein kinase gamma
NCBI Official Symbol
CDC42BPG
NCBI Official Synonym Symbols
DMPK2; MRCKG; HSMDPKIN; KAPPA-200; MRCKgamma
UniProt Protein Name
Serine/threonine-protein kinase MRCK gamma
UniProt Synonym Protein Names
CDC42-binding protein kinase gamma; DMPK-like gamma; Myotonic dystrophy kinase-related CDC42-binding kinase gamma; MRCK gamma; MRCKG; Myotonic dystrophy protein kinase-like gamma; Myotonic dystrophy protein kinase-like alpha
UniProt Gene Name
CDC42BPG
UniProt Synonym Gene Names
MRCK gamma; MRCKG; Myotonic dystrophy protein kinase-like gamma
NCBI Protein Information
serine/threonine-protein kinase MRCK gamma

UniProt Comments for MRCKG

May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation ().

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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