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MRPL4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9605631

Subunit Structure: Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30… More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MRPL4, Polyclonal Antibody
Full Product Name
MRPL4 Antibody
Product Gene Name
anti-MRPL4 antibody
Product Synonym Names
39S ribosomal protein L4; 39S ribosomal protein L4 mitochondrial; CDABP0091; CGI-28; L4mt; mitochondrial; Mitochondrial ribosomal protein L4; MNCb-3848; MRP-L4; MRPL4; RM04_HUMAN
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
MRPL4 antibody detects endogenous levels of total MRPL4
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
MRPL4 peptide (MBS9624348) is used for blocking the activity of MRPL4 antibody (MBS9605631)
Molecular Weight
Observed: 35 kD
Predicted: 35 kDa
3D Structure
ModBase 3D Structure for Q9BYD3
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide
Subcellular Location
Mitochondrion
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Similarity
Pig (100%), Zebrafish (80%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Xenopus (90%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MRPL4 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-MRPL4 antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-MRPL4 antibody

MBS9605631 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-MRPL4 Polyclonal Antibody – Cat# MBS9605631

Western Blot (WB) of anti-MRPL4 antibody

Western blot analysis of extracts from COLO cells, using MRPL4 antibody.
Rabbit Anti-MRPL4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9605631

Other Notes

Small volumes of anti-MRPL4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MRPL4 purchase

MBS9605631 is an antibody targeting MRPL4. Also known as MRPL4 Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MRPL4. It may not necessarily be applicable to this product.

NCBI GI #
22547136
NCBI GeneID
51073
NCBI Accession #
NP_057040.2
UniProt Primary Accession #
Q9BYD3
UniProt Secondary Accession #
Q9BW07; Q9H4N2; Q9Y317; A6NNV7
UniProt Related Accession #
Q9BYD3
GenBank Nucleotide #
NM_015956.2
NCBI Official Full Name
39S ribosomal protein L4, mitochondrial isoform a
NCBI Official Synonym Full Names
mitochondrial ribosomal protein L4
NCBI Official Symbol
MRPL4
NCBI Official Synonym Symbols
L4mt; CGI-28
UniProt Protein Name
39S ribosomal protein L4, mitochondrial
UniProt Synonym Protein Names
Mitochondrial large ribosomal subunit protein uL4m
Protein Family
39S ribosomal protein
UniProt Gene Name
MRPL4
UniProt Synonym Gene Names
L4mt; MRP-L4
NCBI Protein Information
39S ribosomal protein L4, mitochondrial

NCBI Summary for MRPL4

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms. [provided by RefSeq, Jul 2008]
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Research Articles on MRPL4

1. there is a strong association between allergic rhinitis risk and polymorphisms of MRPL4 and TNF-alpha genes in the Han Chinese population

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MRPL4 antibody

 Products by Pathway  Pathway Diagram
 Ribosome Pathway antibodies  Ribosome Pathway Diagram
 Ribosome Pathway antibodies  Ribosome Pathway Diagram

Diseases associated with anti-MRPL4 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >1 publications with MRPL4 and Necrosis

Organs/Tissues associated with anti-MRPL4 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with MRPL4 and Blood
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