Rabbit Anti-NSUN2 Polyclonal Antibody – Cat# MBS6326901

NSUN2 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the NSUN2 antibody detected the NSUN2 protein (arrow).

NSUN2 Antibody – Rabbit Polyclonal

Name: NSUN2 Antibody; Polyclonal; Rabbit; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6326901
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6326901

Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NSUN2, Polyclonal Antibody

Full Product Name

NSUN2, ID (NSUN2, SAKI, TRM4, tRNA (cytosine(34)-C(5))-methyltransferase, Myc-induced SUN domain-containing protein, NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 ho

Product Gene Name

anti-NSUN2 antibody

Product Synonym Names

NSUN2; SAKI; TRM4; tRNA (cytosine(34)-C(5))-methyltransferase; Myc-induced SUN domain-containing protein; NOL1/NOP2/Sun domain family member 2; Substrate of AIM1/Aurora kinase B; tRNA (cytosine-5-)-methyltransferase; tRNA methyltransferase 4 homolog

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

Purity/Purification

Purified by Protein A Affinity Chromatography.

Preparation and Storage

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

82,393 Da

OMIM

gene 611091

3D Structure

ModBase 3D Structure for Q08J23

Immunogen

NSUN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 426-455 amino acids from the Central region of human NSUN2.

Conjugate

HRP

Note

Preservative Free

Product Categories/Family for anti-NSUN2 antibody

Antibodies; Enzymes; Methyltransferase

Applications Tested/Suitable for anti-NSUN2 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-NSUN2 antibody

WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-NSUN2 antibody

Bailey, S.D., et al. Diabetes Care (2010). In press: Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Frye, M., et al. Cancer Lett. 289(1):71-80(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Hussain, S., et al. J. Cell Biol. 186(1):27-40(2009).

Other Notes

Small volumes of anti-NSUN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NSUN2 purchase

MBS6326901 is an antibody targeting NSUN2. Also known as NSUN2, ID (NSUN2, SAKI, TRM4, tRNA (cytosine(34)-C(5))-methyltransferase, Myc-induced SUN domain-containing protein, NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 ho. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NSUN2. It may not necessarily be applicable to this product.

NCBI GI #

39995082

NCBI GeneID

54888

NCBI Accession #

NP_060225.4

UniProt Primary Accession #

Q08J23

UniProt Secondary Accession #

Q9BVN4; Q9H858; Q9NXD9; A8K529; B2RNR4; B3KP09; B4DQW2; G3V1R4

UniProt Related Accession #

Q08J23

GenBank Nucleotide #

NM_017755.6

NCBI Official Full Name

tRNA (cytosine(34)-C(5))-methyltransferase isoform 1

NCBI Official Synonym Full Names

NOP2/Sun RNA methyltransferase family member 2

NCBI Official Symbol

NSUN2

NCBI Official Synonym Symbols

MISU; MRT5; SAKI; TRM4

UniProt Protein Name

tRNA (cytosine(34)-C(5))-methyltransferase

UniProt Synonym Protein Names

Myc-induced SUN domain-containing protein; Misu; NOL1/NOP2/Sun domain family member 2; Substrate of AIM1/Aurora kinase B; tRNA (cytosine-5-)-methyltransferase; tRNA methyltransferase 4 homolog; hTrm4

Protein Family

tRNA

UniProt Gene Name

NSUN2

UniProt Synonym Gene Names

SAKI; TRM4; Misu; hTrm4

UniProt Entry Name

NSUN2_HUMAN

NCBI Protein Information

tRNA (cytosine(34)-C(5))-methyltransferase

NCBI Summary for NSUN2

This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011]

UniProt Comments for NSUN2

NSUN2: an RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity. Interacts with NPM1 and NCL during interphase. Concentrated in the nucleolus during interphase and translocates to the spindle during mitosis as an RNA-protein complex that includes 18S ribosomal RNA. Defects in NSUN2 are the cause of mental retardation autosomal recessive type 5 (MRT5), a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily. Two human isoforms produced by alternative splicing.

Protein type: EC 2.1.1.203; Methyltransferase; Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 5p15.31

Cellular Component: cytoplasm; nucleolus; spindle; nucleus

Molecular Function: tRNA (cytosine-5-)-methyltransferase activity; tRNA binding

Biological Process: mitosis; cell division; tRNA methylation

Disease: Mental Retardation, Autosomal Recessive 5

Research Articles on NSUN2

1. Upregulation of NSUN2 expression is associated with ovarian cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NSUN2 antibody

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