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NT5C3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9505463

MBS9505463 is an antibody targeting NT5C3. Also known as NT5C3 Antibody. Reactive in Human, Mouse. Host: Rabbit. More details

$300 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NT5C3, Polyclonal Antibody
Full Product Name
NT5C3 Antibody
Product Gene Name
Product Synonym Names
Cytosolic 5'-nucleotidase III; cN-III; Pyrimidine 5'-nucleotidase 1; P5'N-1; P5N-1; PN-I; Uridine 5'-monophosphate hydrolase 1; p36
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Concentration
1 mg/ml (lot specific)
Specificity
NT5C3 Antibody detects endogenous levels of total NT5C3 protein.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Molecular Weight
37 kDa
OMIM
AF312735 mRNA
3D Structure
ModBase 3D Structure for Q9H0P0
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human NT5C3.
Immunogen Range
Nov-60
Applications Tested/Suitable for anti-NT5C3 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-NT5C3 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:40000

Immunohistochemistry (IHC) of anti-NT5C3 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NT5C3 Antibody. The picture on the right is blocked with the synthesized peptide.
Rabbit Anti-NT5C3 Polyclonal Antibody – Cat# MBS9505463

Western Blot (WB) of anti-NT5C3 antibody

Western blot analysis of lysates from Jurkat and COLO205 cells, using NT5C3 Antibody. The lane on the right is blocked with the synthesized peptide.
Rabbit Anti-NT5C3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9505463

Other Notes

Small volumes of anti-NT5C3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NT5C3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cytosolic 5'-nucleotidase 3A isoform 2
NCBI Official Synonym Full Names
5'-nucleotidase, cytosolic IIIA
NCBI Official Symbol
NT5C3A
NCBI Official Synonym Symbols
p36; PN-I; POMP; PSN1; UMPH; NT5C3; P5N-1; UMPH1; hUMP1; P5'N-1; cN-III
UniProt Protein Name
Cytosolic 5'-nucleotidase 3A
UniProt Synonym Protein Names
7-methylguanosine phosphate-specific 5'-nucleotidase
UniProt Gene Name
NT5C3A
UniProt Synonym Gene Names
NT5C3; P5N1; UMPH1; 7-methylguanosine nucleotidase; cN-III; P5'N-1; P5N-1; PN-I
NCBI Protein Information
cytosolic 5'-nucleotidase 3A

NCBI Summary for NT5C3

This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. Mutations in this gene are a cause of hemolytic anemia due to uridine 5-prime monophosphate hydrolase deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and pseudogenes of this gene are located on the long arm of chromosomes 3 and 4. [provided by RefSeq, Mar 2012]
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UniProt Comments for NT5C3

Nucleotidase which shows specific activity towards cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m7GMP) (PubMed:24603684). CMP seems to be the preferred substrate (PubMed:15968458).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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