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PADI1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9130516
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This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PADI1, Polyclonal Antibody
Full Product Name
PADI1 Polyclonal Antibody
Product Gene Name
Product Synonym Names
HPAD10;PAD1;PDI;PDI1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Affinity purification
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
OMIM
607934
3D Structure
ModBase 3D Structure for Q9ULC6
Immunogen
Recombinant protein of human PADI1
Category
Primary antibody
Observed MW
65kDa
Calculated MW
74kDa
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Product Categories/Family for anti-PADI1 antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-PADI1 antibody
Western Blot (WB)
Application Notes for anti-PADI1 antibody
WB: 1:200 - 1:1000

Western Blot (WB) of anti-PADI1 antibody

Western blot analysis of extracts of various cell lines, using PADI1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Rabbit Anti-PADI1 Polyclonal Antibody – Cat# MBS9130516

Other Notes

Small volumes of anti-PADI1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PADI1 purchase

MBS9130516 is an antibody targeting PADI1. Also known as PADI1 Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PADI1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein-arginine deiminase type-1
NCBI Official Synonym Full Names
peptidyl arginine deiminase 1
NCBI Official Symbol
PADI1
NCBI Official Synonym Symbols
PDI; PAD1; PDI1; HPAD10
UniProt Protein Name
Protein-arginine deiminase type-1
UniProt Synonym Protein Names
Peptidylarginine deiminase I; Protein-arginine deiminase type I
UniProt Gene Name
PADI1
UniProt Synonym Gene Names
PAD1; PDI1
UniProt Entry Name
PADI1_HUMAN
NCBI Protein Information
protein-arginine deiminase type-1

NCBI Summary for PADI1

This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type I enzyme is involved in the late stages of epidermal differentiation, where it deiminates filaggrin and keratin K1, which maintains hydration of the stratum corneum, and hence the cutaneous barrier function. This enzyme may also play a role in hair follicle formation. This gene exists in a cluster with four other paralogous genes. [provided by RefSeq, Jul 2008]
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UniProt Comments for PADI1

PADI1: Catalyzes the deimination of arginine residues of proteins. Epidermis, prostate, testis, placenta, spleen and thymus. Belongs to the protein arginine deiminase family.

Protein type: Hydrolase; EC 3.5.3.15

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: cytoplasm

Molecular Function: calcium ion binding; protein-arginine deiminase activity

Biological Process: peptidyl-citrulline biosynthetic process from peptidyl-arginine
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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