• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PARS2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9210250

Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1. More details

$240 / 0.08 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PARS2, Polyclonal Antibody
Full Product Name
PARS2 Antibody (C-term)
Product Gene Name
anti-PARS2 antibody
Product Synonym Names
Probable proline--tRNA ligase; mitochondrial; Prolyl-tRNA synthetase; ProRS; PARS2
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human, mouse
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Concentration
Vial Concentration: 2 (lot specific)
Specificity
This PARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 390-417 amino acids from the C-terminal region of human PARS2.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
PARS2 peptide (MBS9218124) is used for blocking the activity of PARS2 antibody (MBS9210250)
Molecular Weight
53263
Immunogen Sequence Positions
390-417
OMIM
612036
3D Structure
ModBase 3D Structure for Q7L3T8
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Applications Tested/Suitable for anti-PARS2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-PARS2 antibody
WB~~1:1000

Western Blot (WB) of anti-PARS2 antibody

PARS2 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the PARS2 antibody detected the PARS2 protein (arrow).
Rabbit Anti-PARS2 Polyclonal Antibody – Cat# MBS9210250

Western Blot (WB) of anti-PARS2 antibody

Western blot analysis of PARS2 (arrow) using rabbit polyclonal PARS2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PARS2 gene.
Rabbit Anti-PARS2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9210250

Immunohistochemistry (IHC) of anti-PARS2 antibody

PARS2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PARS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-PARS2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9210250

Flow Cytometry (FC/FACS) of anti-PARS2 antibody

PARS2 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PARS2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS9210250

Product References and Citations for anti-PARS2 antibody

Lamesch, P., et al. Genomics 89(3):307-315(2007)
Bonnefond, L., et al. Biochemistry 44(12):4805-4816(2005)

Other Notes

Small volumes of anti-PARS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PARS2 purchase

MBS9210250 is an antibody targeting PARS2. Also known as PARS2 Antibody (C-term). Reactive in Human, mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PARS2. It may not necessarily be applicable to this product.

NCBI GI #
34303926
NCBI GeneID
25973
NCBI Accession #
NP_689481.2
UniProt Primary Accession #
Q7L3T8
UniProt Secondary Accession #
Q9H6S5; Q9UFT1; A8K0W4
UniProt Related Accession #
Q7L3T8
GenBank Nucleotide #
NM_152268.3
NCBI Official Full Name
probable proline--tRNA ligase, mitochondrial
NCBI Official Synonym Full Names
prolyl-tRNA synthetase 2, mitochondrial (putative)
NCBI Official Symbol
PARS2
NCBI Official Synonym Symbols
proRS; MT-PRORS
UniProt Protein Name
Probable proline--tRNA ligase, mitochondrial
UniProt Synonym Protein Names
Prolyl-tRNA synthetase; ProRS
UniProt Gene Name
PARS2
UniProt Synonym Gene Names
ProRS
UniProt Entry Name
SYPM_HUMAN
NCBI Protein Information
probable proline--tRNA ligase, mitochondrial

NCBI Summary for PARS2

This gene encodes a putative member of the class II family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of proline to tRNA molecules. Mutations have been found in this gene in some patients with Alpers syndrome. [provided by RefSeq, Mar 2015]
Show more

UniProt Comments for PARS2

PARS2: Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: Mitochondrial; Ligase; EC 6.1.1.15

Chromosomal Location of Human Ortholog: 1p32.2

Cellular Component: mitochondrial matrix

Molecular Function: proline-tRNA ligase activity; ATP binding

Biological Process: tRNA aminoacylation for protein translation; prolyl-tRNA aminoacylation; gene expression
Show more

Research Articles on PARS2

1. unusual 2.0 A structure showing that ATP directly locks onto and orients two parts of halofuginone (HF) onto human ProRS, so that one part of HF mimics bound proline and the other mimics the 3' end of bound tRNA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PARS2 antibody

 Products by Pathway  Pathway Diagram
 Biotin Metabolism Pathway antibodies  Biotin Metabolism Pathway Diagram
 Biotin Metabolism Pathway antibodies  Biotin Metabolism Pathway Diagram
 Biotin Metabolism Pathway antibodies  Biotin Metabolism Pathway Diagram
 Biotin Metabolism Pathway antibodies  Biotin Metabolism Pathway Diagram
 Biotin Metabolism Pathway antibodies  Biotin Metabolism Pathway Diagram
 Biotin Metabolism Pathway antibodies  Biotin Metabolism Pathway Diagram
 Biotin Metabolism Pathway antibodies  Biotin Metabolism Pathway Diagram

Organs/Tissues associated with anti-PARS2 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1 publications with PARS2 and Liver
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000