Rabbit Anti-PDX1 Polyclonal Antibody – Cat# MBS9131203

Western blot analysis of extracts of various cell lines, using PDX1 Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

PDX1 Antibody – Rabbit Polyclonal

Name: PDX1 Antibody; Polyclonal; Rabbit; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS9131203
Price: 360 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9131203

The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

PDX1, Polyclonal Antibody

Full Product Name

PDX1 Polyclonal Antibody

Product Gene Name

anti-PDX1 antibody

Product Synonym Names

GSF; IDX-1; IPF1; IUF1; MODY4; PAGEN1; PDX-1; STF-1

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse

Purity/Purification

Affinity Purification

Preparation and Storage

Store at 4 degree C. Avoid freeze/thaw cycles.

Molecular Weight

31kDa

OMIM

U35632 mRNA

3D Structure

ModBase 3D Structure for P52945

Immunogen

A synthetic peptide of human PDX1

Storage Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Product Categories/Family for anti-PDX1 antibody

Primary antibody

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-PDX1 antibody

Western Blot (WB), Immunofluorescence (IF)

Application Notes for anti-PDX1 antibody

WB: 1:500 - 1:1000
IF: 1:20 - 1:50

Other Notes

Small volumes of anti-PDX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDX1 purchase

MBS9131203 is an antibody targeting PDX1. Also known as PDX1 Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PDX1. It may not necessarily be applicable to this product.

NCBI GI #

1708540

NCBI GeneID

3651

NCBI Accession #

P52945.1

UniProt Primary Accession #

P52945

UniProt Secondary Accession #

O60594; Q5VYW2

UniProt Related Accession #

P52945

NCBI Official Full Name

Pancreas/duodenum homeobox protein 1

NCBI Official Synonym Full Names

pancreatic and duodenal homeobox 1

NCBI Official Symbol

PDX1

NCBI Official Synonym Symbols

GSF; IPF1; IUF1; IDX-1; MODY4; PDX-1; STF-1; PAGEN1

UniProt Protein Name

Pancreas/duodenum homeobox protein 1

UniProt Synonym Protein Names

Glucose-sensitive factor; GSF; Insulin promoter factor 1; IPF-1; Insulin upstream factor 1; IUF-1; Islet/duodenum homeobox-1; IDX-1; Somatostatin-transactivating factor 1; STF-1

Protein Family

Pancreas/duodenum homeobox protein

UniProt Gene Name

PDX1

UniProt Synonym Gene Names

IPF1; STF1; PDX-1; GSF; IPF-1; IUF-1; IDX-1; STF-1

NCBI Protein Information

pancreas/duodenum homeobox protein 1

UniProt Comments for PDX1

PDX1: Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. Interacts with the basic helix-loop-helix domains of TCF3(E47) and NEUROD1 and with HMG-I(Y). Interacts with SPOP. Interacts with the methyltransferase SETD7. Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells). Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 13q12.2

Cellular Component: nucleoplasm

Molecular Function: transcription factor activity

Biological Process: detection of glucose; generation of precursor metabolites and energy; insulin secretion; nitric oxide mediated signal transduction; organ morphogenesis; positive regulation of transcription from RNA polymerase II promoter

Disease: Diabetes Mellitus, Noninsulin-dependent; Diabetes Mellitus, Permanent Neonatal; Maturity-onset Diabetes Of The Young, Type 4; Pancreatic Agenesis 1

Research Articles on PDX1

1. Pdx1 and MafA play crucial roles in the pancreas and maintain mature beta-cell function. Our results showed that the expression of Pdx1 and MafA were significantly upregulated after a sleeve gastrectomy for morbid obesity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PDX1 antibody

Reference Product	PubMed Publications
MAFA antibody	>99 publications with PDX1 and MAFA
INS antibody	>60 publications with PDX1 and INS
FOXA2 antibody	>56 publications with PDX1 and FOXA2
NEUROD1 antibody	>46 publications with PDX1 and NEUROD1
FOXO1 antibody	>22 publications with PDX1 and FOXO1
HNF1A antibody	>15 publications with PDX1 and HNF1A
ONECUT1 antibody	>8 publications with PDX1 and ONECUT1
GCG antibody	>7 publications with PDX1 and GCG
SST antibody	>6 publications with PDX1 and SST
ATF3 antibody	>2 publications with PDX1 and ATF3

Pathways associated with anti-PDX1 antibody

Products by Pathway	Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies	FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
Insulin Secretion Pathway antibodies	Insulin Secretion Pathway Diagram
Maturity Onset Diabetes Of The Young Pathway antibodies	Maturity Onset Diabetes Of The Young Pathway Diagram
Maturity Onset Diabetes Of The Young Pathway antibodies	Maturity Onset Diabetes Of The Young Pathway Diagram
Regulation Of Beta-cell Development Pathway antibodies	Regulation Of Beta-cell Development Pathway Diagram
Regulation Of Gene Expression In Beta Cells Pathway antibodies	Regulation Of Gene Expression In Beta Cells Pathway Diagram
Regulation Of Gene Expression In Early Pancreatic Precursor Cells Pathway antibodies	Regulation Of Gene Expression In Early Pancreatic Precursor Cells Pathway Diagram
Type II Diabetes Mellitus Pathway antibodies	Type II Diabetes Mellitus Pathway Diagram
Type II Diabetes Mellitus Pathway antibodies	Type II Diabetes Mellitus Pathway Diagram

Diseases associated with anti-PDX1 antibody

Disease Name	Pubmed Publications
Diabetes Mellitus Antibodies	>202 publications with PDX1 and Diabetes Mellitus
Pancreatic Neoplasms Antibodies	>102 publications with PDX1 and Pancreatic Neoplasms
Hyperglycemia Antibodies	>101 publications with PDX1 and Hyperglycemia
Diabetes Mellitus, Experimental Antibodies	>71 publications with PDX1 and Diabetes Mellitus, Experimental
Diabetes Mellitus, Type 2 Antibodies	>51 publications with PDX1 and Diabetes Mellitus, Type 2
Insulin Resistance Antibodies	>40 publications with PDX1 and Insulin Resistance
Glucose Intolerance Antibodies	>32 publications with PDX1 and Glucose Intolerance
Hyperinsulinism Antibodies	>21 publications with PDX1 and Hyperinsulinism
Inflammation Antibodies	>17 publications with PDX1 and Inflammation
Necrosis Antibodies	>14 publications with PDX1 and Necrosis

Organs/Tissues associated with anti-PDX1 antibody

Organ/Tissue Name	Pubmed Publications
Pancreas Antibodies	>697 publications with PDX1 and Pancreas
Stomach Antibodies	>30 publications with PDX1 and Stomach
Intestine Antibodies	>25 publications with PDX1 and Intestine