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PGAM2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6332948

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating… More details

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Product Overview

Online Datasheet in PDF
Product Name
PGAM2, Polyclonal Antibody
Full Product Name
PGAM2, NT (PGAM2, PGAMM, Phosphoglycerate mutase 2, BPG-dependent PGAM 2, Muscle-specific phosphoglycerate mutase, Phosphoglycerate mutase isozyme M) (APC)
Product Gene Name
anti-PGAM2 antibody
Product Synonym Names
PGAM2; PGAMM; Phosphoglycerate mutase 2; BPG-dependent PGAM 2; Muscle-specific phosphoglycerate mutase; Phosphoglycerate mutase isozyme M
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
28,766 Da
OMIM
261670
3D Structure
ModBase 3D Structure for P15259
Immunogen
PGAM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-49 amino acids from the N-terminal region of human PGAM2.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-PGAM2 antibody

Antibodies; Enzymes
Applications Tested/Suitable for anti-PGAM2 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-PGAM2 antibody
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PGAM2 antibody

PGAM2 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PGAM2 antibody detected the PGAM2 protein (arrow).
Rabbit Anti-PGAM2 Polyclonal Antibody – Cat# MBS6332948

Western Blot (WB) of anti-PGAM2 antibody

PGAM2 Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the PGAM2 antibody detected the PGAM2 protein (arrow).
Rabbit Anti-PGAM2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6332948

Immunohistochemistry (IHC) of anti-PGAM2 antibody

PGAM2 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PGAM2 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-PGAM2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6332948

Product References and Citations for anti-PGAM2 antibody

Hadjigeorgiou, G.M., et al. Neuromuscul. Disord. 9 (6-7), 399-402 (1999): Tsujino, S., et al. Am. J. Hum. Genet. 52(3):472-477(1993) Castella-Escola, J., et al. Gene 91(2):225-232(1990) Castella-Escola, J., et al. Hum. Genet. 84(2):210-212(1990) Tsujino, S., et al. J. Biol. Chem. 264(26):15334-15337(1989).

Other Notes

Small volumes of anti-PGAM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PGAM2 purchase

MBS6332948 is an antibody targeting PGAM2. Also known as PGAM2, NT (PGAM2, PGAMM, Phosphoglycerate mutase 2, BPG-dependent PGAM 2, Muscle-specific phosphoglycerate mutase, Phosphoglycerate mutase isozyme M) (APC). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PGAM2. It may not necessarily be applicable to this product.

NCBI GI #
50593010
NCBI GeneID
5224
NCBI Accession #
NP_000281.2
UniProt Primary Accession #
P15259
UniProt Related Accession #
P15259
GenBank Nucleotide #
NM_000290.3
NCBI Official Full Name
phosphoglycerate mutase 2
NCBI Official Synonym Full Names
phosphoglycerate mutase 2 (muscle)
NCBI Official Symbol
PGAM2
NCBI Official Synonym Symbols
GSD10; PGAMM; PGAM-M
UniProt Protein Name
Phosphoglycerate mutase 2
UniProt Synonym Protein Names
BPG-dependent PGAM 2; Muscle-specific phosphoglycerate mutase; Phosphoglycerate mutase isozyme M; PGAM-M
Protein Family
Phosphoglycerate mutase
UniProt Gene Name
PGAM2
UniProt Synonym Gene Names
PGAMM; PGAM-M
UniProt Entry Name
PGAM2_HUMAN
NCBI Protein Information
phosphoglycerate mutase 2; BPG-dependent PGAM 2; muscle-specific phosphoglycerate mutase; phosphoglycerate mutase isozyme M

NCBI Summary for PGAM2

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq, Sep 2009]
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UniProt Comments for PGAM2

PGAM2: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). A metabolic disorder characterized by myoglobinuria, increased serum creatine kinase levels, decreased phosphoglycerate mutase activity, myalgia, muscle pain, muscle cramps and excercise intolerance. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Phosphatase (non-protein); Isomerase; EC 5.4.2.11; EC 5.4.2.4; EC 3.1.3.13

Chromosomal Location of Human Ortholog: 7p13-p12

Cellular Component: nucleus; cytosol

Molecular Function: phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; cofactor binding; 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity

Biological Process: response to mercury ion; striated muscle contraction; glycolysis; dephosphorylation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; spermatogenesis; gluconeogenesis

Disease: Glycogen Storage Disease X
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Research Articles on PGAM2

1. These results reveal a mechanism of PGAM2 regulation and NADPH homeostasis in response to oxidative stress that impacts cell proliferation and tumor growth.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PGAM2 antibody

 Reference Product  PubMed Publications
 GPI antibody  >3 publications with PGAM2 and GPI
 ALDOA antibody  >1 publications with PGAM2 and ALDOA
 BPGM antibody  >1 publications with PGAM2 and BPGM
 ENO1 antibody  >1 publications with PGAM2 and ENO1
 PGK1 antibody  >1 publications with PGAM2 and PGK1

Pathways associated with anti-PGAM2 antibody

 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram

Diseases associated with anti-PGAM2 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >3 publications with PGAM2 and Necrosis
 Fibrosis Antibodies  >2 publications with PGAM2 and Fibrosis
 Abnormalities, Multiple Antibodies  >1 publications with PGAM2 and Abnormalities, Multiple
 Heart Failure Antibodies  >1 publications with PGAM2 and Heart Failure
 Weight Gain Antibodies  >1 publications with PGAM2 and Weight Gain
 Nervous System Diseases Antibodies  >1 publications with PGAM2 and Nervous System Diseases
 Death Antibodies  >1 publications with PGAM2 and Death
 Liver Diseases Antibodies  >1 publications with PGAM2 and Liver Diseases
 Kidney Diseases Antibodies  >1 publications with PGAM2 and Kidney Diseases
 Dimauro disease Antibodies  >1 publications with PGAM2 and Dimauro disease

Organs/Tissues associated with anti-PGAM2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >14 publications with PGAM2 and Muscle
 Heart Antibodies  >3 publications with PGAM2 and Heart
 Lung Antibodies  >3 publications with PGAM2 and Lung
 Embryonic Tissue Antibodies  >2 publications with PGAM2 and Embryonic Tissue
 Bone Antibodies  >1 publications with PGAM2 and Bone
 Brain Antibodies  >1 publications with PGAM2 and Brain
 Testis Antibodies  >1 publications with PGAM2 and Testis
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