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PGP9.5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6495017

UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is… More details

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Product Overview

Online Datasheet in PDF
Product Name
PGP9.5, Polyclonal Antibody
Full Product Name
PGP9.5, NT (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1, UCH-L1, Ubiquitin Thiolesterase L1, Neuron Cytoplasmic Protein 9.5, UCHL1) (Biotin)
Product Gene Name
anti-PGP9.5 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Specificity
Recognizes human PGP9.5. Species Crossreactivity: mouse
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
24.8 kDa (223 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
OMIM
191342
3D Structure
ModBase 3D Structure for P09936
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 16-46 in the N-terminal region of human PGP9.5, UniProt Accession #P09936. Species sequence homology: bovine and porcine
Conjugate
Biotin
Note
Preservative Free

Product Categories/Family for anti-PGP9.5 antibody

Antibodies; Ubiquitin
Applications Tested/Suitable for anti-PGP9.5 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PGP9.5 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PGP9.5 antibody

Western Blot analysis of UCHL1 (arrow) of 293 cell lysates (2ug/lane) usingMBS6010792. Lane 1: non-transfected Lane 2: transiently transfected with the UCHL1 gene (Origene Technologies).
Rabbit Anti-PGP9.5 Polyclonal Antibody – Cat# MBS6495017

Western Blot (WB) of anti-PGP9.5 antibody

Western Blot analysis of UCHL1-V31 in CEM(lane 1), Jurkat (lane 2), Y79(lane 3) cell line and mouse brain tissue(lane 4) lysates (35ug/lane) usingMBS6010792. UCHL1 (arrow) was detected using the purified Pab.
Rabbit Anti-PGP9.5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6495017

Immunohistochemistry (IHC) of anti-PGP9.5 antibody

Formalin-fixed and paraffin-embedded human brain tissue usingMBS6010792, followed by peroxidase-conjugated secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6010792 for immunohistochemistry
Rabbit Anti-PGP9.5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6495017

Immunofluorescence (IF) of anti-PGP9.5 antibody

Confocal immunofluorescent analysis of NCI-H460 cell using MBS6010792 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-PGP9.5 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6495017

Flow Cytometry (FC/FACS) of anti-PGP9.5 antibody

Flow cytometric analysis of NCI-H460 cells using MBS6010792 (right histogram) compared to a negative control cell (left histogram). IgG (FITC) goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PGP9.5 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6495017

Product References and Citations for anti-PGP9.5 antibody

1. Maraganore, D.M., et al., Mov Disord 18(6):631-636 (2003). 2. Nishikawa, K., et al., Biochem. Biophys. Res. Commun. 304(1):176-183 (2003). 3. Liu, Y., et al., Cell 111(2):209-218 (2002). 4. Caballero, O.L., et al., Oncogene 21(19):3003-3010 (2002). 5. Saigoh, K., et al., Nat. Genet. 23(1):47-51 (1999).

Other Notes

Small volumes of anti-PGP9.5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PGP9.5 purchase

MBS6495017 is an antibody targeting PGP9.5. Also known as PGP9.5, NT (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1, UCH-L1, Ubiquitin Thiolesterase L1, Neuron Cytoplasmic Protein 9.5, UCHL1) (Biotin). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PGP9.5. It may not necessarily be applicable to this product.

NCBI GI #
21361091
NCBI GeneID
7345
NCBI Accession #
NP_004172
UniProt Primary Accession #
P09936
UniProt Secondary Accession #
Q4W5K6; Q71UM0
UniProt Related Accession #
P09936
GenBank Nucleotide #
NM_004181.4
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase isozyme L1
NCBI Official Synonym Full Names
ubiquitin C-terminal hydrolase L1
NCBI Official Symbol
UCHL1
NCBI Official Synonym Symbols
NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Synonym Protein Names
Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1
Protein Family
Ubiquitin carboxyl-terminal hydrolase
UniProt Gene Name
UCHL1
UniProt Synonym Gene Names
UCH-L1; PGP9.5
UniProt Entry Name
UCHL1_HUMAN
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L1

NCBI Summary for PGP9.5

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]
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UniProt Comments for PGP9.5

UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family.

Protein type: Cell development/differentiation; EC 3.4.19.12; Ligase; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; cell soma; axon; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; omega peptidase activity; ubiquitin binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; alpha-2A adrenergic receptor binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; cell proliferation; negative regulation of MAP kinase activity; protein deubiquitination; axon transport of mitochondrion; axon target recognition; eating behavior; neuromuscular process; sensory perception of pain; muscle fiber development; adult walking behavior

Disease: Neurodegeneration With Optic Atrophy, Childhood-onset; Parkinson Disease 5, Autosomal Dominant
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Research Articles on PGP9.5

1. UCHL1 localizes to TTAGGG repeats at telomeres and interstitial telomeric sequences. A weak or transient interaction between UCHL1 and the shelterin complex was confirmed by immunoprecipitation and proximity ligation assays.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PGP9.5 antibody

 Reference Product  PubMed Publications
 HSP90AA1 antibody  >2 publications with PGP9.5 and HSP90AA1
 CDKN1B antibody  >2 publications with PGP9.5 and CDKN1B
 NEDD8 antibody  >1 publications with PGP9.5 and NEDD8
 LAMP2 antibody  >1 publications with PGP9.5 and LAMP2
 HSPA8 antibody  >1 publications with PGP9.5 and HSPA8

Pathways associated with anti-PGP9.5 antibody

 Products by Pathway  Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 Deubiquitination Pathway antibodies  Deubiquitination Pathway Diagram
 Hepatitis C And Hepatocellular Carcinoma Pathway antibodies  Hepatitis C And Hepatocellular Carcinoma Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Parkinson's Disease Pathway antibodies  Parkinson's Disease Pathway Diagram
 Parkinsons Disease Pathway antibodies  Parkinsons Disease Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Proteasome Degradation Pathway antibodies  Proteasome Degradation Pathway Diagram
 UCH Proteinases Pathway antibodies  UCH Proteinases Pathway Diagram

Diseases associated with anti-PGP9.5 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >263 publications with PGP9.5 and Neoplasms
 Nervous System Diseases Antibodies  >192 publications with PGP9.5 and Nervous System Diseases
 Brain Diseases Antibodies  >142 publications with PGP9.5 and Brain Diseases
 Inflammation Antibodies  >46 publications with PGP9.5 and Inflammation
 Adenocarcinoma Antibodies  >29 publications with PGP9.5 and Adenocarcinoma
 Peripheral Nervous System Diseases Antibodies  >29 publications with PGP9.5 and Peripheral Nervous System Diseases
 Cardiovascular Diseases Antibodies  >27 publications with PGP9.5 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >27 publications with PGP9.5 and Disease Models, Animal
 Necrosis Antibodies  >19 publications with PGP9.5 and Necrosis
 Nerve Degeneration Antibodies  >18 publications with PGP9.5 and Nerve Degeneration

Organs/Tissues associated with anti-PGP9.5 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >173 publications with PGP9.5 and Nerve
 Brain Antibodies  >106 publications with PGP9.5 and Brain
 Skin Antibodies  >85 publications with PGP9.5 and Skin
 Bone Antibodies  >59 publications with PGP9.5 and Bone
 Lymph Antibodies  >55 publications with PGP9.5 and Lymph
 Lung Antibodies  >36 publications with PGP9.5 and Lung
 Mouth Antibodies  >27 publications with PGP9.5 and Mouth
 Testis Antibodies  >20 publications with PGP9.5 and Testis
 Uterus Antibodies  >19 publications with PGP9.5 and Uterus
 Embryonic Tissue Antibodies  >18 publications with PGP9.5 and Embryonic Tissue
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