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POLE2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9609763

MBS9609763 is an antibody targeting POLE2. Also known as POLE2 Antibody. Reactive in Human, Mouse. Host: Rabbit. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POLE2, Polyclonal Antibody
Full Product Name
POLE2 Antibody
Product Gene Name
Product Synonym Names
DNA polymerase epsilon subunit 2; DNA polymerase epsilon subunit B; DNA polymerase II subunit 2; DPE 2; DPE2; DPOE2_HUMAN; POLE 2; POLE2; Polymerase (DNA directed) epsilon 2 (p59 subunit); Polymerase (DNA directed) epsilon 2; polymerase (DNA directed), epsilon 2, accessory subunit; Polymerase, DNA, epsilon-2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
POLE2 peptide (MBS9628467) is used for blocking the activity of POLE2 antibody (MBS9609763)
Molecular Weight
60 kDa
3D Structure
ModBase 3D Structure for P56282
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human POLE2, corresponding to a region within C-terminal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-POLE2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-POLE2 antibody
WB: 1:1000-3000
IHC: 1:50-1:200
The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

Western Blot (WB) of anti-POLE2 antibody

Western blot analysis of POLE2 using LOVO whole cell lysates
Rabbit Anti-POLE2 Polyclonal Antibody – Cat# MBS9609763

Immunohistochemistry (IHC) of anti-POLE2 antibody

MBS9609763 at 1/100 staining rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.
Rabbit Anti-POLE2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9609763

Other Notes

Small volumes of anti-POLE2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POLE2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA polymerase epsilon subunit 2 isoform 2
NCBI Official Synonym Full Names
DNA polymerase epsilon 2, accessory subunit
NCBI Official Symbol
POLE2
NCBI Official Synonym Symbols
DPE2
UniProt Protein Name
DNA polymerase epsilon subunit 2
UniProt Synonym Protein Names
DNA polymerase II subunit 2; DNA polymerase epsilon subunit B
Protein Family
UniProt Gene Name
POLE2
UniProt Synonym Gene Names
DPE2
NCBI Protein Information
DNA polymerase epsilon subunit 2

NCBI Summary for POLE2

DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency. [provided by RefSeq, Jan 2017]
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UniProt Comments for POLE2

Participates in DNA repair and in chromosomal DNA replication.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-POLE2 antibody

Organs/Tissues associated with anti-POLE2 antibody

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