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PON2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6335884

PON2 is a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular… More details

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Product Overview

Online Datasheet in PDF
Product Name
PON2, Polyclonal Antibody
Full Product Name
PON2, ID (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (AP)
Product Gene Name
Product Synonym Names
PON2; Serum paraoxonase/arylesterase 2; Aromatic esterase 2; Serum aryldialkylphosphatase 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
43.5 kDa
OMIM
602447
3D Structure
ModBase 3D Structure for Q15165
Immunogen
PON2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the Central region of human PON2.
Conjugate
AP
Note
Preservative Free

Product Categories/Family for anti-PON2 antibody

Applications Tested/Suitable for anti-PON2 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-PON2 antibody
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PON2 antibody

PON2 Antibody (Center) western blot analysis in HepG2 cell line lysates (15ug/lane).This demonstrates the PON2 antibody detected PON2 protein (arrow).
Rabbit Anti-PON2 Polyclonal Antibody – Cat# MBS6335884

Western Blot (WB) of anti-PON2 antibody

PON2 Antibody (Center) western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the PON2 antibody detected PON2 protein (arrow).
Rabbit Anti-PON2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6335884

Immunohistochemistry (IHC) of anti-PON2 antibody

PON2 antibody(Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PON2 antibody(Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-PON2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6335884

Immunofluorescence (IF) of anti-PON2 antibody

Confocal immunofluorescent analysis of PON2 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-PON2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6335884

Product References and Citations for anti-PON2 antibody

Sanghera, D.K., et al. Am. J. Hum. Genet. 62(1):36-44(1998). Hegele, R.A., et al. J. Clin. Endocrinol. Metab. 82(10):3373-3377(1997). Primo-Parmo, S.L., et al. Genomics 33(3):498-507(1996).

Other Notes

Small volumes of anti-PON2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PON2 purchase

MBS6335884 is an antibody targeting PON2. Also known as PON2, ID (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (AP). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PON2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serum paraoxonase/arylesterase 2 isoform 1
NCBI Official Synonym Full Names
paraoxonase 2
NCBI Official Symbol
PON2
UniProt Protein Name
Serum paraoxonase/arylesterase 2
UniProt Synonym Protein Names
Aromatic esterase 2; A-esterase 2; Serum aryldialkylphosphatase 2
UniProt Gene Name
PON2
UniProt Synonym Gene Names
PON 2; A-esterase 2
UniProt Entry Name
PON2_HUMAN
NCBI Protein Information
serum paraoxonase/arylesterase 2; A-esterase 2; PON 2; aromatic esterase 2; paraoxonase nirs; serum aryldialkylphosphatase 2

NCBI Summary for PON2

This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for PON2

PON2: Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Belongs to the paraoxonase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.81; Hydrolase; EC 3.1.1.2; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: mitochondrion; lysosome; plasma membrane; extracellular region; nucleus

Molecular Function: identical protein binding; arylesterase activity; metal ion binding

Biological Process: response to oxidative stress; aromatic compound catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PON2 antibody

Pathways associated with anti-PON2 antibody

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