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PRIM2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9131630
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This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PRIM2, Polyclonal Antibody
Full Product Name
PRIM2 Polyclonal Antibody
Product Gene Name
Product Synonym Names
PRIM2A; p58
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Molecular Weight
59kDa
OMIM
X74331 mRNA
3D Structure
ModBase 3D Structure for P49643
Immunogen
Recombinant protein of human PRIM2
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Product Categories/Family for anti-PRIM2 antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-PRIM2 antibody
Western Blot (WB)
Application Notes for anti-PRIM2 antibody
WB: 1:500 - 1:2000

Western Blot (WB) of anti-PRIM2 antibody

Western blot analysis of extracts of various cell lines, using PRIM2 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Rabbit Anti-PRIM2 Polyclonal Antibody – Cat# MBS9131630

Western Blot (WB) of anti-PRIM2 antibody

Western blot analysis of extracts of mouse spleen, using PRIM2 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Rabbit Anti-PRIM2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9131630

Other Notes

Small volumes of anti-PRIM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRIM2 purchase

MBS9131630 is an antibody targeting PRIM2. Also known as PRIM2 Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PRIM2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
DNA primase large subunit
NCBI Official Synonym Full Names
primase (DNA) subunit 2
NCBI Official Symbol
PRIM2
NCBI Official Synonym Symbols
p58; PRIM2A
UniProt Protein Name
DNA primase large subunit
UniProt Synonym Protein Names
DNA primase 58 kDa subunit; p58
UniProt Gene Name
PRIM2
UniProt Synonym Gene Names
PRIM2A; p58
NCBI Protein Information
DNA primase large subunit

UniProt Comments for PRIM2

PRI2: synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The replication of DNA is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components.

Protein type: EC 2.7.7.-; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transferase

Chromosomal Location of Human Ortholog: 6p11.2

Cellular Component: alpha DNA polymerase:primase complex; nucleoplasm

Molecular Function: DNA primase activity; DNA-directed DNA polymerase activity; single-stranded DNA binding

Biological Process: DNA replication initiation; G1/S transition of mitotic cell cycle; telomere maintenance via recombination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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