Rabbit Anti-PRKX Polyclonal Antibody – Cat# MBS6495366

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PRKX antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PRKX Antibody – Rabbit Polyclonal

Name: PRKX Antibody; Polyclonal; Rabbit; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6495366
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6495366

MBS6495366 is an antibody targeting PRKX. Also known as PRKX, CT (Protein Kinase PKX1, Serine/Threonine-protein Kinase PRKX, PKX1) (PE). Reactive in Human, Mouse. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PRKX, Polyclonal Antibody

Full Product Name

PRKX, CT (Protein Kinase PKX1, Serine/Threonine-protein Kinase PRKX, PKX1) (PE)

Product Gene Name

anti-PRKX antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

Purity/Purification

Purified by Protein G Affinity Chromatography.

Specificity

Recognizes human PRKX. Species Crossreactivity: mouse.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

40,896 Da

Chromosome Location

Chromosome: X; NC_000023.10 (3522384..3631675, complement). Location: Xp22.3

OMIM

300083

3D Structure

ModBase 3D Structure for P51817

Immunogen

Synthetic peptide selected from the C-terminal region of human PRKX (KLH).

Conjugate

Note

Preservative Free

Special Handling

Light sensitive

Product Categories/Family for anti-PRKX antibody

Antibodies; Protein Kinases

Applications Tested/Suitable for anti-PRKX antibody

Immunohistochemistry (IHC), FLISA, Western Blot (WB)

Application Notes for anti-PRKX antibody

IHC: 1:10-1:50
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Product References and Citations for anti-PRKX antibody

1. Li, X., et al., Proc. Natl. Acad. Sci. USA 99(14):9260-9265 (2002). 2. Klink, A., et al., Hum. Mol. Genet. 4(5):869-878 (1995).

Other Notes

Small volumes of anti-PRKX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PRKX. It may not necessarily be applicable to this product.

NCBI GI #

4826948

NCBI GeneID

5613

NCBI Accession #

NP_005035.1

UniProt Primary Accession #

P51817

UniProt Related Accession #

P51817

GenBank Nucleotide #

NM_005044.4

NCBI Official Full Name

cAMP-dependent protein kinase catalytic subunit PRKX

NCBI Official Synonym Full Names

protein kinase, X-linked

NCBI Official Symbol

PRKX

NCBI Official Synonym Symbols

PKX1

UniProt Protein Name

cAMP-dependent protein kinase catalytic subunit PRKX

UniProt Synonym Protein Names

Protein kinase PKX1

Protein Family

cAMP-dependent protein kinase

UniProt Gene Name

PRKX

UniProt Synonym Gene Names

PKX1; PrKX; Protein kinase X; Protein kinase X-linked

UniProt Entry Name

PRKX_HUMAN

NCBI Protein Information

cAMP-dependent protein kinase catalytic subunit PRKX; protein kinase X; protein kinase PKX1; protein kinase X-linked; serine/threonine-protein kinase PRKX

NCBI Summary for PRKX

This gene encodes a serine threonine protein kinase that has similarity to the catalytic subunit of cyclic AMP dependent protein kinases. The encoded protein is developmentally regulated and may be involved in renal epithelial morphogenesis. This protein may also be involved in macrophage and granulocyte maturation. Abnormal recombination between this gene and a related pseudogene on chromosome Y is a frequent cause of sex reversal disorder in XX males and XY females. Pseudogenes of this gene are found on chromosomes X, 15 and Y. [provided by RefSeq, Feb 2010]

UniProt Comments for PRKX

PRKX: Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation. A chromosomal aberration involving PRKX is a cause of sex reversal disorder. Translocation t(X;Y)(p22;p11) with PRKY. Chromosomal translocations proximal to PRKY account for about 30% of the cases of sex reversal disorder in XX males and XY females. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.1; AGC group; PKA family

Chromosomal Location of Human Ortholog: Xp22.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; cAMP-dependent protein kinase activity; ATP binding

Biological Process: regulation of cell adhesion; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; endothelial cell proliferation; endothelial cell migration; myeloid cell differentiation; angiogenesis; cell adhesion; regulation of cell migration; cell-substrate adhesion

Research Articles on PRKX

1. findings reveals a major role of PRKX, TTBK2 and RSK4 in triggering Sunitinib resistance formation; data suggest transcriptional regulation of these kinases together with other proteins might play an important role in formation of Sunitinib resistance by affecting transcription factors

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PRKX antibody

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Diseases associated with anti-PRKX antibody

Disease Name	Pubmed Publications
Disorders of Sex Development Antibodies	>4 publications with PRKX and Disorders of Sex Development
Kidney Diseases Antibodies	>4 publications with PRKX and Kidney Diseases
Neoplasms Antibodies	>2 publications with PRKX and Neoplasms
Infertility, Female Antibodies	>1 publications with PRKX and Infertility, Female
Kidney Neoplasms Antibodies	>1 publications with PRKX and Kidney Neoplasms
Nerve Degeneration Antibodies	>1 publications with PRKX and Nerve Degeneration
Nervous System Diseases Antibodies	>1 publications with PRKX and Nervous System Diseases

Organs/Tissues associated with anti-PRKX antibody

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>9 publications with PRKX and Kidney
Heart Antibodies	>6 publications with PRKX and Heart
Lung Antibodies	>4 publications with PRKX and Lung
Blood Antibodies	>4 publications with PRKX and Blood
Embryonic Tissue Antibodies	>4 publications with PRKX and Embryonic Tissue
Brain Antibodies	>3 publications with PRKX and Brain
Liver Antibodies	>2 publications with PRKX and Liver
Testis Antibodies	>2 publications with PRKX and Testis
Bone Antibodies	>2 publications with PRKX and Bone
Pancreas Antibodies	>1 publications with PRKX and Pancreas