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REQU Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9604841

Function: May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.Similarity: Belongs to the requiem/DPF family. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
REQU, Polyclonal Antibody
Full Product Name
REQU Antibody
Product Gene Name
anti-REQU antibody
Product Synonym Gene Name
DPF2
Product Synonym Names
Apoptosis response zinc finger protein; BAF45D; BRG1-associated factor 45D; D4; D4 zinc and double PHD fingers family 2; double PHD fingers family 2; DPF 2; DPF2; MGC10180; Protein requiem; REQ; REQU_HUMAN; Requiem; Requiem apoptosis response zinc finger; UBI D4; ubi-d4; UBID 4; UBID4; zinc and double PHD fingers family 2; Zinc finger protein ubi d4; Zinc finger protein ubi-d4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
peptide affinity purification
Concentration
1mg/ml (lot specific)
Specificity
REQU Antibody detects endogenous levels of total REQU.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
REQU peptide (MBS9623558) is used for blocking the activity of REQU antibody (MBS9604841)
Molecular Weight
44 KD
3D Structure
ModBase 3D Structure for Q92785
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human REQU, corresponding to a region within the internal amino acids.
Important
For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-REQU antibody
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes for anti-REQU antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
ELISA(peptide): 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Western Blot (WB) of anti-REQU antibody

Western blot analysis of extracts from HT-29 cells, using REQU antibody.
Rabbit Anti-REQU Polyclonal Antibody – Cat# MBS9604841

Immunofluorescence (IF) of anti-REQU antibody

MBS9604841 staining HT29 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-REQU Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9604841

Other Notes

Small volumes of anti-REQU antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for REQU purchase

MBS9604841 is an antibody targeting REQU. Also known as REQU Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for REQU. It may not necessarily be applicable to this product.

NCBI GI #
5454004
NCBI GeneID
5977
NCBI Accession #
NP_006259.1
UniProt Primary Accession #
Q92785
UniProt Secondary Accession #
A8K7C9; B4DT58
UniProt Related Accession #
Q92785
GenBank Nucleotide #
NM_006268.4
NCBI Official Full Name
zinc finger protein ubi-d4 isoform 1
NCBI Official Synonym Full Names
double PHD fingers 2
NCBI Official Symbol
DPF2
NCBI Official Synonym Symbols
REQ; CSS7; UBID4; ubi-d4
UniProt Protein Name
Zinc finger protein ubi-d4
UniProt Synonym Protein Names
Apoptosis response zinc finger protein; BRG1-associated factor 45D; BAF45D; D4, zinc and double PHD fingers family 2; Protein requiem
Protein Family
Required for meiotic nuclear division protein
UniProt Gene Name
DPF2
UniProt Synonym Gene Names
BAF45D; REQ; UBID4; BAF45D
NCBI Protein Information
zinc finger protein ubi-d4

NCBI Summary for REQU

The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors. [provided by RefSeq, Jul 2008]
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UniProt Comments for REQU

May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.

Research Articles on REQU

1. Authors show that DPF2 interacts with the acetylated tails of both histones 3 and 4 via bipartite binding pockets on the DPF2 surface. Blocking these interactions through targeted mutagenesis of DPF2 abolishes its recruitment to target chromatin regions as well as its ability to prevent myeloid differentiation in vivo.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-REQU antibody

 Reference Product  PubMed Publications
 RELB antibody  >2 publications with REQU and RELB

Pathways associated with anti-REQU antibody

 Products by Pathway  Pathway Diagram
 TNF-alpha/NF-kB Signaling Pathway antibodies  TNF-alpha/NF-kB Signaling Pathway Diagram

Diseases associated with anti-REQU antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >1 publications with REQU and Necrosis

Organs/Tissues associated with anti-REQU antibody

 Organ/Tissue Name  Pubmed Publications
 Ovary Antibodies  >2 publications with REQU and Ovary
 Spleen Antibodies  >1 publications with REQU and Spleen
 Embryonic Tissue Antibodies  >1 publications with REQU and Embryonic Tissue
 Thymus Antibodies  >1 publications with REQU and Thymus
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