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SART3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9609146

Function: U6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassembly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation (PubMed:12032085). Also binds U6atac… More details

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Product Overview

Online Datasheet in PDF
Product Name
SART3, Polyclonal Antibody
Full Product Name
SART3 Antibody
Product Gene Name
anti-SART3 antibody
Product Synonym Names
DSAP1; hSART 3; hSART-3; P100; P110; p110(nrb); RP11 13G14; SART 3; SART-3; SART3; SART3_HUMAN; Squamous cell carcinoma antigen recognized by T cells 3; Squamous cell carcinoma antigen recognized by T-cells 3; Tat interacting protein of 110 kDa; Tat-interacting protein of 110 kDa; Tip110
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
SART3 antibody detects endogenous levels of total SART3
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
SART3 peptide (MBS9627851) is used for blocking the activity of SART3 antibody (MBS9609146)
Molecular Weight
Observed: 110 kDa
Predicted: 110 kDa
3D Structure
ModBase 3D Structure for Q15020
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human SART3
Subcellular Location
Cytoplasm. Nucleus Speckle. Localized in speckles. Expressed in the nucleus of all of the malignant tumor cell lines tested and the majority of cancer tissues with various histologies, including squamous cell carcinomas (SCC), adenocarcinomas, melanomas and leukemias cells. However, this protein is undetectable in the nucleus of any cell lines of nonmalignant cells or normal tissues, except for the testis. Expressed in the cytoplasm of all the proliferating cells, including normal and malignant cells, but not in normal tissues, except for the testis and the fetal liver.
Tissue Specificity
Ubiquitously expressed.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (90%), Bovine (90%), Horse (90%), Sheep (90%), Rabbit (90%), Dog (90%), Xenopus (80%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-SART3 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-SART3 antibody
WB: 1:1000-3000
IHC: 1:200

Western Blot (WB) of anti-SART3 antibody

Western blot analysis SART3 using HeLa whole cell lysates
Rabbit Anti-SART3 Polyclonal Antibody – Cat# MBS9609146

Immunohistochemistry (IHC) of anti-SART3 antibody

MBS9609146 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-SART3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9609146

Other Notes

Small volumes of anti-SART3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SART3 purchase

MBS9609146 is an antibody targeting SART3. Also known as SART3 Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SART3. It may not necessarily be applicable to this product.

NCBI GI #
7661952
NCBI GeneID
9733
NCBI Accession #
NP_055521.1
UniProt Primary Accession #
Q15020
UniProt Secondary Accession #
Q2M2H0; Q58F06; Q8IUS1; Q96J95; A8K2E4; B7ZKM0
UniProt Related Accession #
Q15020
GenBank Nucleotide #
NM_014706.4
NCBI Official Full Name
squamous cell carcinoma antigen recognized by T-cells 3
NCBI Official Synonym Full Names
spliceosome associated factor 3, U4/U6 recycling protein
NCBI Official Symbol
SART3
NCBI Official Synonym Symbols
P100; p110; DSAP1; TIP110; p110(nrb); RP11-13G14
UniProt Protein Name
Squamous cell carcinoma antigen recognized by T-cells 3
UniProt Synonym Protein Names
Tat-interacting protein of 110 kDa
Protein Family
Squamous cell carcinoma antigen
UniProt Gene Name
SART3
UniProt Synonym Gene Names
KIAA0156ImportedManual assertion inferred from database entriesiEMBL:BAA09929.1; TIP1101 PublicationManual assertion based on opinion iniRef.2; Tip1101 PublicationManual assertion based on opinion iniRef.2
NCBI Protein Information
squamous cell carcinoma antigen recognized by T-cells 3

NCBI Summary for SART3

The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. [provided by RefSeq, Jul 2008]
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UniProt Comments for SART3

U6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassembly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation (PubMed:12032085). Also binds U6atac snRNPs and may function as a recycling factor for U4atac/U6atac spliceosomal snRNP, an initial step in the assembly of U12-type spliceosomal complex. The U12-type spliceosomal complex plays a role in the splicing of introns with non-canonical splice sites (PubMed:14749385). May also function as a substrate-targeting factor for deubiquitinases like USP4 and USP15. Recruits USP4 to ubiquitinated PRPF3 within the U4/U5/U6 tri-snRNP complex, promoting PRPF3 deubiquitination and thereby regulating the spliceosome U4/U5/U6 tri-snRNP spliceosomal complex disassembly (PubMed:20595234). May also recruit the deubiquitinase USP15 to histone H2B and mediate histone deubiquitination, thereby regulating gene expression and/or DNA repair (PubMed:24526689). May play a role in hematopoiesis probably through transcription regulation of specific genes including MYC ().
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Research Articles on SART3

1. Data suggest that ZIP, USP39, Prp24/p100/SART3, and Prp43 associate to form complex instrumental in spliceosome assembly; ZIP regulates pre-mRNA splicing of USP39 independent of RNA binding; stable 35S tri-snRNP particles are enriched in Cajal body. (ZIP = zinc finger and G-patch domain-containing protein; USP39 = ubiquitin specific peptidase 39; Prp43 = RNA helicase Prp43)
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SART3 antibody

 Reference Product  PubMed Publications
 LSM2 antibody  >1 publications with SART3 and LSM2
 USP4 antibody  >1 publications with SART3 and USP4
 PRPF3 antibody  >1 publications with SART3 and PRPF3

Diseases associated with anti-SART3 antibody

 Disease Name  Pubmed Publications
 Porokeratosis, disseminated superficial actinic 1 Antibodies  >3 publications with SART3 and Porokeratosis, disseminated superficial actinic 1
 Headache Antibodies  >1 publications with SART3 and Headache
 Nervous System Diseases Antibodies  >1 publications with SART3 and Nervous System Diseases
 Uterine Cervical Neoplasms Antibodies  >1 publications with SART3 and Uterine Cervical Neoplasms
 Central Nervous System Diseases Antibodies  >1 publications with SART3 and Central Nervous System Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with SART3 and Carcinoma, Hepatocellular
 Brain Diseases Antibodies  >1 publications with SART3 and Brain Diseases
 Liver Diseases Antibodies  >1 publications with SART3 and Liver Diseases
 Kidney Diseases Antibodies  >1 publications with SART3 and Kidney Diseases

Organs/Tissues associated with anti-SART3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >26 publications with SART3 and Blood
 Prostate Antibodies  >5 publications with SART3 and Prostate
 Liver Antibodies  >4 publications with SART3 and Liver
 Lung Antibodies  >3 publications with SART3 and Lung
 Skin Antibodies  >2 publications with SART3 and Skin
 Bone Antibodies  >2 publications with SART3 and Bone
 Brain Antibodies  >2 publications with SART3 and Brain
 Thymus Antibodies  >1 publications with SART3 and Thymus
 Spleen Antibodies  >1 publications with SART3 and Spleen
 Kidney Antibodies  >1 publications with SART3 and Kidney
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