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SETDB2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9604080

Description: Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SETDB2, Polyclonal Antibody
Full Product Name
SETDB2 Antibody
Product Gene Name
Product Synonym Names
C13orf4; Chromosome 13 open reading frame 4; Chronic lymphocytic leukemia deletion region 8; Chronic lymphocytic leukemia deletion region gene 8; Chronic lymphocytic leukemia deletion region gene 8 protein; Clld8; CLLL8; Gm293; H3-K9-HMTase; Histone H3-K9 methyltransferase; Histone-lysine N-methyltransferase SETDB2; KMT1F; Lysine N-methyltransferase 1F; Probable histone-lysine N-methyltransferase H3 lysine-9 specific; SEB2; SET domain bifurcated 2; SET domain protein, bifurcated, 2; SETB2_HUMAN; Setdb2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
SETDB2 antibody detects endogenous levels of total SETDB2
Antibody/Peptide Pairs
SETDB2 peptide (MBS9622797) is used for blocking the activity of SETDB2 antibody (MBS9604080)
Molecular Weight
Observed: 120 kDa
Predicted: 82 kDa
3D Structure
ModBase 3D Structure for Q96T68
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide
Subcellular Location
Nucleus. Chromosome.
Tissue Specificity
Ubiquitous. Highest expression in heart, testis and ovary.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Zebrafish (100%), Bovine (90%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-SETDB2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-SETDB2 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Immunohistochemistry (IHC) of anti-SETDB2 antibody

MBS9604080 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-SETDB2 Polyclonal Antibody – Cat# MBS9604080

Western Blot (WB) of anti-SETDB2 antibody

Western blot analysis of SETDB2 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-SETDB2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9604080

Other Notes

Small volumes of anti-SETDB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SETDB2 purchase

MBS9604080 is an antibody targeting SETDB2. Also known as SETDB2 Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SETDB2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone-lysine N-methyltransferase SETDB2 isoform b
NCBI Official Synonym Full Names
SET domain bifurcated 2
NCBI Official Symbol
SETDB2
NCBI Official Synonym Symbols
CLLD8; CLLL8; KMT1F; C13orf4
UniProt Protein Name
Histone-lysine N-methyltransferase SETDB2
UniProt Synonym Protein Names
Chronic lymphocytic leukemia deletion region gene 8 protein; Lysine N-methyltransferase 1F; SET domain bifurcated 2
UniProt Gene Name
SETDB2
UniProt Synonym Gene Names
C13orf4; CLLD8; KMT1F
NCBI Protein Information
histone-lysine N-methyltransferase SETDB2

NCBI Summary for SETDB2

This gene encodes a member of a family of proteins that contain a methyl-CpG-binding domain (MBD) and a SET domain and function as histone methyltransferases. This protein is recruited to heterochromatin and plays a role in the regulation of chromosome segregation. This region is commonly deleted in chronic lymphocytic leukemia. Naturally-occuring readthrough transcription occurs from this gene to the downstream PHF11 (PHD finger protein 11) gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
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UniProt Comments for SETDB2

Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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