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SIRT3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6499577

MBS6499577 is an antibody targeting SIRT3. Also known as SIRT3, CT (Sirtuin 3, Sirtuin-3, Sirtuin Silent Mating Type Information Regulation 2 Homolog 3 (S. cerevisiae), Sirtuin Type 3, HGNC:14931, hSIRT3, Mitochondrial Nicotinamide Adenine… More details

$1060 / 0.2 mL +1 FREE 8GB USB
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SIRT3, Polyclonal Antibody
Full Product Name
SIRT3, CT (Sirtuin 3, Sirtuin-3, Sirtuin Silent Mating Type Information Regulation 2 Homolog 3 (S. cerevisiae), Sirtuin Type 3, HGNC:14931, hSIRT3, Mitochondrial Nicotinamide Adenine Dinucleotide-dependent Deacetylase, NAD-dependent Deacetylase Sirtuin 3
Product Gene Name
anti-SIRT3 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein G Affinity Chromatography.
Specificity
Recognizes human SIRT3. Species Crossreactivity: mouse.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
28,567 Da
OMIM
604481
3D Structure
ModBase 3D Structure for Q9NTG7
Immunogen
Peptide selected from the C-terminal region of human Sirt3.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-SIRT3 antibody

Antibodies; Sirtuins
Applications Tested/Suitable for anti-SIRT3 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-SIRT3 antibody
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-SIRT3 antibody

Western blot analysis of SIRT3 antibody in Y79 cell line lysates (35ug/lane). SIRT3 (arrow) was detected using the purified Pab.
Rabbit Anti-SIRT3 Polyclonal Antibody – Cat# MBS6499577

Western Blot (WB) of anti-SIRT3 antibody

Sirt3 Antibody (G265) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Sirt3 antibody detected the Sirt3 protein (arrow).
Rabbit Anti-SIRT3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6499577

Immunohistochemistry (IHC) of anti-SIRT3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-SIRT3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6499577

Product References and Citations for anti-SIRT3 antibody

1. Onyango, P., et al., Proc. Natl. Acad. Sci. USA 99(21):13653-13658 (2002). 2. Schwer, B., et al., J. Cell Biol. 158(4):647-657 (2002). 3. Frye, R.A., Biochem. Biophys. Res. Commun. 273(2):793-798 (2000). 4. Frye, R.A., Biochem. Biophys. Res. Commun. 260(1):273-279 (1999). 5. Nagalingam R. Sundaresan, et al. Mol. Cell. Biol., Oct 2008; 28: 6384-6401.

Other Notes

Small volumes of anti-SIRT3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SIRT3. It may not necessarily be applicable to this product.

NCBI GI #
6912660
NCBI GeneID
23410
NCBI Accession #
NP_036371
UniProt Primary Accession #
Q9NTG7
UniProt Secondary Accession #
Q9Y6E8; B7Z5U6
UniProt Related Accession #
Q9NTG7
GenBank Nucleotide #
NM_012239.5
NCBI Official Full Name
NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform a
NCBI Official Synonym Full Names
sirtuin 3
NCBI Official Symbol
SIRT3
NCBI Official Synonym Symbols
SIR2L3
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-3, mitochondrial
UniProt Synonym Protein Names
Regulatory protein SIR2 homolog 3; SIR2-like protein 3
Protein Family
NAD-dependent protein deacetylase sirtuin
UniProt Gene Name
SIRT3
UniProt Synonym Gene Names
SIR2L3; hSIRT3
UniProt Entry Name
SIR3_HUMAN
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-3, mitochondrial; sir2-like 3; sirtuin type 3; SIR2-like protein 3; regulatory protein SIR2 homolog 3; NAD-dependent deacetylase sirtuin-3, mitochondrial; silent mating type information regulation 2, S.cerevisiae

NCBI Summary for SIRT3

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for SIRT3

SIRT3: a soluble mitochondrial NAD-dependent protein deacetylase. Can deacetylate and thereby activate a central metabolic regulator in the mitochondrial matrix, glutamate dehydrogenase. Mitochondrial proteins are hyperacetylated in SIRT3-deficient mice, but not in SIRT4 or SIRT5 deficient mice. SIRT3-deficient mice have no obvious metabolic phenotype, and the process of adaptive thermogenesis is normal. Can deacetylate and activate isocitrate dehydrogenase 2, an enzyme that participates in regulation of the citric acid cycle and the regeneration of antioxidants. Inhibited by nicotinamide, but not by trichostatin A. Its unprocessed form is enzymatically inactive. It is processed by mitochondrial processing peptidase (MPP) in the mitochondrial matrix to give an enzymatically active 28 kDa product.

Protein type: Deacetylase; EC 3.5.1.-; Mitochondrial

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: mitochondrion; membrane; mitochondrial matrix

Molecular Function: protein binding; enzyme binding; zinc ion binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NAD+ ADP-ribosyltransferase activity

Biological Process: mitochondrion organization and biogenesis; protein amino acid ADP-ribosylation; protein amino acid deacetylation; organelle organization and biogenesis; aerobic respiration; aging
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Research Articles on SIRT3

1. In endothelial cells, SIRT3 stabilizes FOXO3 via deacetylation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SIRT3 antibody

 Reference Product  PubMed Publications
 SIRT5 antibody  >59 publications with SIRT3 and SIRT5
 FOXO3 antibody  >12 publications with SIRT3 and FOXO3
 IDH2 antibody  >6 publications with SIRT3 and IDH2
 UCP1 antibody  >4 publications with SIRT3 and UCP1
 STK11 antibody  >2 publications with SIRT3 and STK11
 ALDH2 antibody  >2 publications with SIRT3 and ALDH2

Pathways associated with anti-SIRT3 antibody

 Products by Pathway  Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Energy Metabolism Pathway antibodies  Energy Metabolism Pathway Diagram
 Mitochondrial Biogenesis Pathway antibodies  Mitochondrial Biogenesis Pathway Diagram
 Organelle Biogenesis And Maintenance Pathway antibodies  Organelle Biogenesis And Maintenance Pathway Diagram
 Signaling Events Mediated By HDAC Class I Pathway antibodies  Signaling Events Mediated By HDAC Class I Pathway Diagram
 Signaling Events Mediated By HDAC Class III Pathway antibodies  Signaling Events Mediated By HDAC Class III Pathway Diagram
 Transcriptional Activation Of Mitochondrial Biogenesis Pathway antibodies  Transcriptional Activation Of Mitochondrial Biogenesis Pathway Diagram

Diseases associated with anti-SIRT3 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >36 publications with SIRT3 and Cardiovascular Diseases
 Fatty Liver Antibodies  >21 publications with SIRT3 and Fatty Liver
 Inflammation Antibodies  >19 publications with SIRT3 and Inflammation
 Liver Diseases Antibodies  >19 publications with SIRT3 and Liver Diseases
 Hypertrophy Antibodies  >17 publications with SIRT3 and Hypertrophy
 Disease Models, Animal Antibodies  >17 publications with SIRT3 and Disease Models, Animal
 Breast Neoplasms Antibodies  >11 publications with SIRT3 and Breast Neoplasms
 Cardiomegaly Antibodies  >9 publications with SIRT3 and Cardiomegaly
 Hypertension Antibodies  >8 publications with SIRT3 and Hypertension
 Carcinoma, Squamous Cell Antibodies  >8 publications with SIRT3 and Carcinoma, Squamous Cell

Organs/Tissues associated with anti-SIRT3 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >64 publications with SIRT3 and Muscle
 Heart Antibodies  >48 publications with SIRT3 and Heart
 Blood Antibodies  >32 publications with SIRT3 and Blood
 Adipose Tissue Antibodies  >25 publications with SIRT3 and Adipose Tissue
 Brain Antibodies  >17 publications with SIRT3 and Brain
 Connective Tissue Antibodies  >16 publications with SIRT3 and Connective Tissue
 Kidney Antibodies  >14 publications with SIRT3 and Kidney
 Lung Antibodies  >10 publications with SIRT3 and Lung
 Pancreas Antibodies  >6 publications with SIRT3 and Pancreas
 Prostate Antibodies  >5 publications with SIRT3 and Prostate
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