SLC22A3 Antibody – Rabbit Polyclonal

Figure 1. Western blot analysis of SLC22A3 using anti-SLC22A3 antibody (MBS1753676).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates
Lane 2: human Hela whole cell lysates
Lane 3: mouse skeletal muscle tissue lysates
Lane 4: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SLC22A3 antigen affinity purified polyclonal antibody (Catalog # MBS1753676) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SLC22A3 at approximately 61KD. The expected band size for SLC22A3 is at 61KD.

Figure 2. Flow Cytometry analysis of Hela cells using anti-SLC22A3 antibody (MBS1753676).
Overlay histogram showing Hela cells stained with MBS1753676 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC22A3 Antibody (MBS1753676, 1μg/1x10⁶ cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.