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SOX9 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9601899

Description: SOX9 Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Defects in SOX9 are the cause of campomelic dysplasia… More details

$325 / 0.1 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
SOX9, Polyclonal Antibody
Full Product Name
SOX9 Antibody
Product Gene Name
Product Synonym Names
campomelic dysplasia autosomal sex reversal; CMD 1; CMD1; CMPD 1; CMPD1; SOX 9; Sox9; SOX9_HUMAN; SRA 1; SRA1; SRXX2; SRXY10; SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal; SRY (sex determining region Y) box 9; SRY (sex determining region Y)-box 9; SRY (sex-determining region Y)-box 9 protein; SRY related HMG box gene 9; Transcription factor SOX 9; Transcription factor SOX-9; transcription factor SOX9
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
SOX9 antibody detects endogenous levels of total SOX9
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
SOX9 peptide (MBS9617452) is used for blocking the activity of SOX9 antibody (MBS9601899)
Molecular Weight
Observed: 56 kDa
Predicted: 57 kDa
3D Structure
ModBase 3D Structure for P48436
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human SOX9
Subcellular Location
Nucleus.
Predicted Cross Reactivity
Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (92%), Xenopus (92%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-SOX9 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunoflurescene (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA (EIA)
Application Notes for anti-SOX9 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
IP: 1/200

Immunofluorescence (IF) of anti-SOX9 antibody

MBS9601899 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-SOX9 Polyclonal Antibody – Cat# MBS9601899

Immunofluorescene (IF) of anti-SOX9 antibody

MBS9601899 staining Hep G2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-SOX9 Polyclonal Antibody, Immunofluorescene (IF) – Cat# MBS9601899

Immunohistochemistry (IHC) of anti-SOX9 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using SOX9 Antibody
Rabbit Anti-SOX9 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9601899

Western Blot (WB) of anti-SOX9 antibody

Western blot analysis of SOX9 expression in NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-SOX9 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9601899

Product References and Citations for anti-SOX9 antibody

Yu M, Yi SQ, Wu YR, Sun HL, Song FF, Wang JW; Journal: Int J Biochem Cell Biol. Ddit3 suppresses the differentiation of mouse chondroprogenitor cells.

Other Notes

Small volumes of anti-SOX9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SOX9 purchase

MBS9601899 is an antibody targeting SOX9. Also known as SOX9 Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SOX9. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcription factor SOX-9
NCBI Official Synonym Full Names
SRY-box 9
NCBI Official Symbol
SOX9
NCBI Official Synonym Symbols
CMD1; SRA1; CMPD1; SRXX2; SRXY10
UniProt Protein Name
Transcription factor SOX-9
Protein Family
UniProt Gene Name
SOX9
NCBI Protein Information
transcription factor SOX-9

NCBI Summary for SOX9

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008]
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UniProt Comments for SOX9

Transcriptional regulator that plays a role in chondrocytes differentiation and skeletal development (PubMed:24038782). Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with ZNF219 ().

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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