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SPCS3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6500368

MBS6500368 is an antibody targeting SPCS3. Also known as SPCS3, CT (Signal Peptidase Complex Subunit 3, Microsomal Signal Peptidase 22/23kD Subunit, SPase 22/23kD Subunit, SPC22/23, SPC22, UNQ1841/PRO3567) (MaxLight 750). Reactive in Human, Mouse. Host:… More details

$1025 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SPCS3, Polyclonal Antibody
Full Product Name
SPCS3, CT (Signal Peptidase Complex Subunit 3, Microsomal Signal Peptidase 22/23kD Subunit, SPase 22/23kD Subunit, SPC22/23, SPC22, UNQ1841/PRO3567) (MaxLight 750)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human SPCS3. Species Crossreactivity: mouse.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
20kDa
3D Structure
ModBase 3D Structure for P61009
Immunogen
Synthetic peptide selected from the C-terminal region of human SPCS3 (KLH).
Conjugate
MaxLight750
Note
Preservative Free

Product Categories/Family for anti-SPCS3 antibody

Applications Tested/Suitable for anti-SPCS3 antibody
Western Blot (WB), FLISA
Application Notes for anti-SPCS3 antibody
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-SPCS3 antibody

Western blot analysis of SPCS3 antibody (C-term) in mouse liver tissue lysates (35ug/lane). SPCS3 (arrow) was detected using the purified Pab.
Rabbit Anti-SPCS3 Polyclonal Antibody – Cat# MBS6500368

Immunohistochemistry (IHC) of anti-SPCS3 antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SPCS3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-SPCS3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6500368

Flow Cytometry (FC/FACS) of anti-SPCS3 antibody

SPCS3 Antibody (C-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-SPCS3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6500368

Product References and Citations for anti-SPCS3 antibody

1. Clark, H.F., Genome Res. 13(10), 2265-2270 (2003).

Other Notes

Small volumes of anti-SPCS3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPCS3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
signal peptidase complex subunit 3
NCBI Official Synonym Full Names
signal peptidase complex subunit 3
NCBI Official Symbol
SPCS3
NCBI Official Synonym Symbols
SPC3; PRO3567; YLR066W; SPC22/23
UniProt Protein Name
Signal peptidase complex subunit 3
UniProt Synonym Protein Names
Microsomal signal peptidase 22/23 kDa subunit
UniProt Gene Name
SPCS3
UniProt Synonym Gene Names
SPC22
UniProt Entry Name
SPCS3_HUMAN
NCBI Protein Information
signal peptidase complex subunit 3

UniProt Comments for SPCS3

SPCS3: Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Belongs to the SPCS3 family.

Protein type: Membrane protein, integral; Cofactor and Vitamin Metabolism - biotin; Protease; Amino Acid Metabolism - lysine degradation; EC 3.4.-.-

Chromosomal Location of Human Ortholog: 4q34.2

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane; signal peptidase complex

Molecular Function: peptidase activity

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; signal peptide processing; translation; gene expression; proteolysis; regulation of insulin secretion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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