• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

USF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6361773

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
USF1, Polyclonal Antibody
Full Product Name
USF1, ID (USF1, BHLHB11, USF, Upstream stimulatory factor 1, Class B basic helix-loop-helix protein 11, Major late transcription factor 1) (AP)
Product Gene Name
anti-USF1 antibody
Product Synonym Names
USF1; BHLHB11; USF; Upstream stimulatory factor 1; Class B basic helix-loop-helix protein 11; Major late transcription factor 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
27,372 Da
OMIM
gene 602491
3D Structure
ModBase 3D Structure for P22415
Immunogen
USF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-201 amino acids from the Central region of human USF1.
Conjugate
AP
Note
Preservative Free

Product Categories/Family for anti-USF1 antibody

Antibodies; Transcription Factors
Applications Tested/Suitable for anti-USF1 antibody
Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-USF1 antibody
IF: 1:10-50
ELISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-USF1 antibody

USF1 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the USF1 antibody detected the USF1 protein (arrow).
Rabbit Anti-USF1 Polyclonal Antibody – Cat# MBS6361773

Flow Cytometry (FC/FACS) of anti-USF1 antibody

USF1 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-USF1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6361773

Immunofluorescence (IF) of anti-USF1 antibody

Fluorescent confocal image of SK-BR-3 cell stained with USF1 Antibody (Center). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with USF1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).USF1 immunoreactivity is localized to nucleus significantly.
Rabbit Anti-USF1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6361773

Immunohistochemistry (IHC) of anti-USF1 antibody

USF1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USF1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-USF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6361773

Product References and Citations for anti-USF1 antibody

Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Bussiere, F.I., et al. Cell. Microbiol. 12(8):1124-1133(2010). Naukkarinen, J., et al. Circ Cardiovasc Genet 2(5):522-529(2009). Singmann, P., et al. Obes Facts 2(4):235-242(2009).

Other Notes

Small volumes of anti-USF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for USF1 purchase

MBS6361773 is an antibody targeting USF1. Also known as USF1, ID (USF1, BHLHB11, USF, Upstream stimulatory factor 1, Class B basic helix-loop-helix protein 11, Major late transcription factor 1) (AP). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for USF1. It may not necessarily be applicable to this product.

NCBI GI #
6005934
NCBI GeneID
7391
NCBI Accession #
NP_009053.1
UniProt Primary Accession #
P22415
UniProt Secondary Accession #
Q5SY46; Q7Z5Y1; B2RBZ4
UniProt Related Accession #
P22415
GenBank Nucleotide #
NM_007122.4
NCBI Official Full Name
upstream stimulatory factor 1 isoform 1
NCBI Official Synonym Full Names
upstream transcription factor 1
NCBI Official Symbol
USF1
NCBI Official Synonym Symbols
UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11
UniProt Protein Name
Upstream stimulatory factor 1
UniProt Synonym Protein Names
Class B basic helix-loop-helix protein 11; bHLHb11; Major late transcription factor 1
Protein Family
Upstream stimulatory factor
UniProt Gene Name
USF1
UniProt Synonym Gene Names
BHLHB11; USF; bHLHb11
UniProt Entry Name
USF1_HUMAN
NCBI Protein Information
upstream stimulatory factor 1; major late transcription factor 1; class B basic helix-loop-helix protein 11

UniProt Comments for USF1

USF1: Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as an homodimer or a heterodimer (USF1/USF2). Interacts with varicella-zoster virus IE62 protein.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1q22-q23

Cellular Component: nucleoplasm; transcription factor complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; protein homodimerization activity; sequence-specific DNA binding; protein heterodimerization activity; histone deacetylase binding; double-stranded DNA binding; bHLH transcription factor binding; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; lipid homeostasis; glucose metabolic process; late viral mRNA transcription; glucose homeostasis; regulation of transcription from RNA polymerase II promoter; negative regulation of fibrinolysis; cellular response to insulin stimulus; regulation of transcription from RNA polymerase II promoter by glucose; response to hypoxia; regulation of transcription by carbon catabolites; positive regulation of transcription from RNA polymerase II promoter by glucose; positive regulation of transcription from RNA polymerase II promoter; response to UV

Disease: Hyperlipidemia, Combined, 1
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-USF1 antibody

 Reference Product  PubMed Publications
 USF2 antibody  >172 publications with USF1 and USF2
 SERPINE1 antibody  >9 publications with USF1 and SERPINE1
 GTF2I antibody  >6 publications with USF1 and GTF2I
 ETS1 antibody  >4 publications with USF1 and ETS1
 PRMT1 antibody  >1 publications with USF1 and PRMT1

Pathways associated with anti-USF1 antibody

 Products by Pathway  Pathway Diagram
 Signaling Mediated By P38-alpha And P38-beta Pathway antibodies  Signaling Mediated By P38-alpha And P38-beta Pathway Diagram

Diseases associated with anti-USF1 antibody

 Disease Name  Pubmed Publications
 Hyperlipidemia, Familial Combined Antibodies  >27 publications with USF1 and Hyperlipidemia, Familial Combined
 Cardiovascular Diseases Antibodies  >21 publications with USF1 and Cardiovascular Diseases
 Liver Neoplasms Antibodies  >12 publications with USF1 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >12 publications with USF1 and Carcinoma, Hepatocellular
 Atherosclerosis Antibodies  >12 publications with USF1 and Atherosclerosis
 Heart Diseases Antibodies  >10 publications with USF1 and Heart Diseases
 Nervous System Diseases Antibodies  >10 publications with USF1 and Nervous System Diseases
 Myocardial Ischemia Antibodies  >10 publications with USF1 and Myocardial Ischemia
 Fatty Liver Antibodies  >9 publications with USF1 and Fatty Liver
 Inflammation Antibodies  >8 publications with USF1 and Inflammation

Organs/Tissues associated with anti-USF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >69 publications with USF1 and Liver
 Blood Antibodies  >62 publications with USF1 and Blood
 Brain Antibodies  >30 publications with USF1 and Brain
 Heart Antibodies  >22 publications with USF1 and Heart
 Testis Antibodies  >13 publications with USF1 and Testis
 Ovary Antibodies  >11 publications with USF1 and Ovary
 Bone Marrow Antibodies  >9 publications with USF1 and Bone Marrow
 Thyroid Antibodies  >7 publications with USF1 and Thyroid
 Embryonic Tissue Antibodies  >6 publications with USF1 and Embryonic Tissue
 Pancreas Antibodies  >6 publications with USF1 and Pancreas
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000