Rabbit Anti-USP25 Polyclonal Antibody – Cat# MBS9629837

USP25 Antibody – Rabbit Polyclonal

Name: USP25 Antibody; Polyclonal; Rabbit; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS9629837
Price: 325 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9629837

MBS9629837 is an antibody targeting USP25. Also known as USP25 Antibody. Reactive in Human, Mouse. Host: Rabbit. More details

$325 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

USP25, Polyclonal Antibody

Full Product Name

USP25 Antibody

Product Gene Name

anti-USP25 antibody

Product Synonym Names

Deubiquitinating enzyme 25; Ubiquitin carboxyl-terminal hydrolase 25; Ubiquitin specific peptidase 25; ubiquitin specific protease 25; ubiquitin specific protease USP25; Ubiquitin thiolesterase 25; Ubiquitin-specific-processing protease 25; UBP25_HUMAN; USP on chromosome 21; USP21; USP25

Clonality

Polyclonal

Isotype

Rabbit IgG

Host

Rabbit

Species Reactivity

Human, Mouse

Form/Format

Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Purity/Purification

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Concentration

1mg/ml (lot specific)

Specificity

USP25 Antibody detects endogenous levels of USP25.

Preparation and Storage

Store at -20 degree C. Stable for 12 months from date of receipt.

Antibody/Peptide Pairs

USP25 peptide (MBS9632220) is used for blocking the activity of USP25 antibody (MBS9629837)

Molecular Weight

1055

OMIM

604736

3D Structure

ModBase 3D Structure for Q9UHP3

Supply Chain Verification

Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.

Immunogen

A synthesized peptide derived from human USP25.

Conjugation

Unconjugated

Similarity

Belongs to the peptidase C19 family.

Post Translational Modifications

Acetylated.Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially with SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.

Subunit Structure

Homodimer or oligomer. Interacts with ACTA1 (via its C-terminus); the interaction occurs for all isoforms but is strongest for isoform USP25m in muscle differentiating cells. Interacts (isoform USP25m only) with MYBPC1; the interaction prevents proteasomal degradation of MYBPC1. Interacts (isoform USP25m only) with FLNC (via filament repeats 17-18, 20-21 and 24). Interacts with GAPDH. Interacts with SUMO3; the interaction sumoylates efficiently USP25. Interacts with SUMO2; the interaction sumoylates efficiently USP25. Interacts with SUMO1; the interaction only weakly sumoylates USP25. Interacts with SYK; phosphorylates USP25 and regulates USP25 intracellular levels.

Tissue Specificity

Isoform USP25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific.

Function

Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.

Note

ome transient punctuate nuclear location in myotubes during myocyte development.

Product Categories/Family for anti-USP25 antibody

Cytoplasm; Nucleus; Primary

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-USP25 antibody

ELISA (EIA)(peptide)

Application Notes for anti-USP25 antibody

ELISA(peptide): 1:20000-1:40000

Other Notes

Small volumes of anti-USP25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for USP25. It may not necessarily be applicable to this product.

NCBI GI #

302393833

NCBI GeneID

29761

NCBI Accession #

Q9UHP3.4

UniProt Primary Accession #

Q9UHP3

UniProt Secondary Accession #

Q6DHZ9; Q9H9W1; C0LSZ0

UniProt Related Accession #

Q9UHP3

NCBI Official Full Name

Ubiquitin carboxyl-terminal hydrolase 25

NCBI Official Synonym Full Names

ubiquitin specific peptidase 25

NCBI Official Symbol

USP25

NCBI Official Synonym Symbols

USP21

UniProt Protein Name

Ubiquitin carboxyl-terminal hydrolase 25

UniProt Synonym Protein Names

Deubiquitinating enzyme 25; USP on chromosome 21; Ubiquitin thioesterase 25; Ubiquitin-specific-processing protease 25

UniProt Gene Name

USP25

UniProt Synonym Gene Names

USP21

UniProt Entry Name

UBP25_HUMAN

NCBI Protein Information

ubiquitin carboxyl-terminal hydrolase 25; USP on chromosome 21; ubiquitin thioesterase 25; deubiquitinating enzyme 25; ubiquitin thiolesterase 25; ubiquitin-specific processing protease 25

NCBI Summary for USP25

Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008]

UniProt Comments for USP25

USP25: Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Protease; Ubiquitin-specific protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 21q11.2

Cellular Component: cytoplasm; nucleus

Molecular Function: peptidase activity; protein binding; SUMO binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein modification process; proteolysis

Research Articles on USP25

1. These findings indicate that miR-200c exerts tumor-suppressive effects for NSCLC through the suppression of USP25 expression and suggests a new therapeutic application of miR-200c in the treatment of NSCLC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-USP25 antibody

Reference Product	PubMed Publications
SUMO2 antibody	>3 publications with USP25 and SUMO2
MYBPC1 antibody	>2 publications with USP25 and MYBPC1

Diseases associated with anti-USP25 antibody

Disease Name	Pubmed Publications
Lung Neoplasms Antibodies	>2 publications with USP25 and Lung Neoplasms
Neurobehavioral Manifestations Antibodies	>2 publications with USP25 and Neurobehavioral Manifestations
Congenital Abnormalities Antibodies	>2 publications with USP25 and Congenital Abnormalities
Inflammation Antibodies	>2 publications with USP25 and Inflammation
Nervous System Diseases Antibodies	>2 publications with USP25 and Nervous System Diseases
Fatty Liver Antibodies	>1 publications with USP25 and Fatty Liver
Breast Neoplasms Antibodies	>1 publications with USP25 and Breast Neoplasms
Liver Diseases Antibodies	>1 publications with USP25 and Liver Diseases
Fibrosis Antibodies	>1 publications with USP25 and Fibrosis
Embryo Loss Antibodies	>1 publications with USP25 and Embryo Loss

Organs/Tissues associated with anti-USP25 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>4 publications with USP25 and Muscle
Lung Antibodies	>2 publications with USP25 and Lung
Testis Antibodies	>2 publications with USP25 and Testis
Bone Antibodies	>1 publications with USP25 and Bone
Heart Antibodies	>1 publications with USP25 and Heart
Liver Antibodies	>1 publications with USP25 and Liver
Blood Antibodies	>1 publications with USP25 and Blood
Embryonic Tissue Antibodies	>1 publications with USP25 and Embryonic Tissue
Lymph Node Antibodies	>1 publications with USP25 and Lymph Node
Brain Antibodies	>1 publications with USP25 and Brain